LOCUS       V013733                 8460 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013733
VERSION     V013733
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8460)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    55..76
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        91..121
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    129..145
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     153..169
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        190..208
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        236..462
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    690..815
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1308..1541
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1726..1770
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1919..1960
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2037..2154
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2177..2480
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2481..2684
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    2801..2825
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2834..3550
                     /label="EmGFP"
                     /note="Emerald GFP"
     protein_bind    complement(3554..3578)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3631..3672
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     misc_feature    3696..4284
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     promoter        4299..4615
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        4663..4710
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             4729..5124
                     /label="BSD"
                     /note="blasticidin S deaminase"
     LTR             5214..5447
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5519..5653
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5680..5815
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(5836..5854)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5864..5880)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6022..6477
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6503..6607
                     /label="AmpR promoter"
     CDS             6608..7465
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7639..8227
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
       61 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      121 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      181 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
      241 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
      301 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
      361 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
      421 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
      481 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
      541 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
      601 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
      661 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
      721 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
      781 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
      841 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
      901 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
      961 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     1021 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     1081 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     1141 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     1201 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     1261 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     1321 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     1381 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     1441 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     1501 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     1561 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     1621 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     1681 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     1741 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     1801 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     1861 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     1921 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     1981 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg gttaactttt
     2041 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     2101 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttatcgat
     2161 aagcttggga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     2221 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     2281 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     2341 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     2401 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     2461 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     2521 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     2581 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     2641 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
     2701 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgactctaga
     2761 ggatccacta gtccagtgtg gtggaattct gcagatatca acaagtttgt acaaaaaagc
     2821 aggctttaaa accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     2881 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     2941 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     3001 gccctggccc accctcgtga ccaccttcac ctacggcgtg cagtgcttcg cccgctaccc
     3061 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     3121 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     3181 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     3241 catcctgggg cacaagctgg agtacaacta caacagccac aaggtctata tcaccgccga
     3301 caagcagaag aacggcatca aggtgaactt caagacccgc cacaacatcg aggacggcag
     3361 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     3421 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     3481 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     3541 gctgtacaag taaacccagc tttcttgtac aaagtggttg atatccagca cagtggcggc
     3601 cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt
     3661 ctcgattcta cgcgtaccgg ttagtaatga tcgacaatca acctctggat tacaaaattt
     3721 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     3781 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     3841 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     3901 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     3961 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4021 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4081 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     4141 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4201 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4261 tctccctttg ggccgcctcc ccgcctggcg atggtaccgg tgtggaaagt ccccaggctc
     4321 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     4381 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4441 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4501 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     4561 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     4621 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     4681 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagcct
     4741 ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc
     4801 atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact
     4861 ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact
     4921 gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg
     4981 ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc
     5041 aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg
     5101 ccctctggtt atgtgtggga gggctaagca caattcgagc tcggtacctt taagaccaat
     5161 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     5221 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     5281 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     5341 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     5401 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     5461 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     5521 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5581 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     5641 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     5701 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     5761 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     5821 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     5881 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     5941 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     6001 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     6061 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     6121 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     6181 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     6241 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     6301 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     6361 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     6421 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     6481 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     6541 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     6601 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     6661 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     6721 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     6781 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     6841 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     6901 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     6961 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     7021 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     7081 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     7141 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     7201 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     7261 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     7321 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     7381 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     7441 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     7501 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     7561 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7621 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7681 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7741 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     7801 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     7861 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     7921 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     7981 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8041 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8101 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8161 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8221 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8281 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     8341 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     8401 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
//
