LOCUS       V011776                 9351 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011776
VERSION     V011776
KEYWORDS    pLenti6.3 TO V5-DEST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9351)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9351)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1804..1921
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        1944..2246
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2247..2450
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    2452..2470
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2473..2491
                     /gene="tetO"
                     /label="tetracycline repressor TetR binding site"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2532..2656
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2681..2711
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2765..3421
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             3766..4068
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(4112..4236)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4289..4330
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     misc_feature    4354..4942
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     promoter        4957..5273
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        5321..5368
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             5387..5782
                     /label="BSD"
                     /note="blasticidin S deaminase"
     LTR             5872..6105
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6177..6311
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6338..6473
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(6494..6512)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6522..6538)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6680..7135
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7161..7265
                     /label="AmpR promoter"
     CDS             7266..8123
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8297..8885
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    9173..9194
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9209..9239
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9247..9263
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9271..9287
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        9308..9326
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgataag cttgggagtt ccgcttacat aacttacggt aaatggcccg cctggctgac
     1981 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2041 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     2101 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     2161 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     2221 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     2281 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     2341 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     2401 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctccctatca
     2461 gtgatagaga tctccctatc agtgatagag atcgtcgact agtccagtgt ggtggaattc
     2521 tgcagatatc aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata
     2581 tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac
     2641 atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg
     2701 ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc
     2761 taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
     2821 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
     2881 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
     2941 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga
     3001 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
     3061 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat
     3121 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
     3181 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
     3241 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
     3301 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt
     3361 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc
     3421 gtaaagatct ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga
     3481 tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtat
     3541 gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca
     3601 tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc
     3661 tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta
     3721 ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca gtttaaggtt
     3781 tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt
     3841 gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa
     3901 gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc
     3961 accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac
     4021 cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc
     4081 tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca
     4141 gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt
     4201 ttacgtttct cgttcagctt tcttgtacaa agtggttgat atccagcaca gtggcggccg
     4261 ctcgagtcta gaggggccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct
     4321 cgattctacg cgtaccggtt agtaatgatc gacaatcaac ctctggatta caaaatttgt
     4381 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     4441 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     4501 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     4561 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     4621 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     4681 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     4741 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     4801 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     4861 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     4921 tccctttggg ccgcctcccc gcctggcgat ggtaccggtg tggaaagtcc ccaggctccc
     4981 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     5041 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     5101 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     5161 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     5221 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     5281 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
     5341 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagccttt
     5401 gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat
     5461 ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg
     5521 tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc
     5581 tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg
     5641 catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa
     5701 agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc
     5761 ctctggttat gtgtgggagg gctaagcaca attcgagctc ggtaccttta agaccaatga
     5821 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     5881 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     5941 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     6001 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     6061 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     6121 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     6181 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6241 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6301 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     6361 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     6421 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     6481 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     6541 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     6601 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     6661 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6721 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     6781 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     6841 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     6901 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6961 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     7021 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     7081 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     7141 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     7201 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     7261 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     7321 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     7381 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     7441 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     7501 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     7561 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     7621 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     7681 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     7741 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     7801 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     7861 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     7921 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     7981 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     8041 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     8101 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     8161 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     8221 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     8281 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     8341 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8401 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     8461 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8521 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8581 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8641 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     8701 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8761 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8821 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8881 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     8941 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     9001 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     9061 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     9121 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     9181 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     9241 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     9301 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct t
//
