LOCUS       V013940                 9047 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013940
VERSION     V013940
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9047)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    55..76
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        91..121
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    129..145
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     153..169
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        190..208
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        236..462
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    690..815
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1308..1541
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1726..1770
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1919..1960
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2037..2154
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2177..2480
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2481..2684
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    2801..2825
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2834..3420
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             3421..4137
                     /label="EGFP"
                     /note="enhanced GFP"
     protein_bind    complement(4141..4165)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4218..4259
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     misc_feature    4283..4871
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     promoter        4886..5202
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        5250..5297
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             5316..5711
                     /label="BSD"
                     /note="blasticidin S deaminase"
     LTR             5801..6034
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6106..6240
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6267..6402
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(6423..6441)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6451..6467)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6609..7064
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7090..7194
                     /label="AmpR promoter"
     CDS             7195..8052
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8226..8814
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
       61 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      121 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      181 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
      241 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
      301 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
      361 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
      421 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
      481 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
      541 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
      601 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
      661 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
      721 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
      781 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
      841 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
      901 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
      961 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     1021 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     1081 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     1141 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     1201 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     1261 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     1321 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     1381 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     1441 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     1501 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     1561 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     1621 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     1681 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     1741 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     1801 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     1861 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     1921 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     1981 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg gttaactttt
     2041 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     2101 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttatcgat
     2161 aagcttggga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     2221 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     2281 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     2341 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     2401 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     2461 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     2521 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     2581 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     2641 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
     2701 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgactctaga
     2761 ggatccacta gtccagtgtg gtggaattct gcagatatca acaagtttgt acaaaaaagc
     2821 aggctttaaa acccccctct ccctcccccc cccctaacgt tactggccga agccgcttgg
     2881 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     2941 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     3001 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     3061 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     3121 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     3181 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     3241 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     3301 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     3361 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacaacc
     3421 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     3481 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     3541 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     3601 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     3661 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     3721 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     3781 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     3841 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     3901 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     3961 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     4021 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     4081 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     4141 acccagcttt cttgtacaaa gtggttgata tccagcacag tggcggccgc tcgagtctag
     4201 agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc
     4261 gtaccggtta gtaatgatcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     4321 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     4381 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     4441 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     4501 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     4561 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     4621 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     4681 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     4741 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     4801 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     4861 cgcctccccg cctggcgatg gtaccggtgt ggaaagtccc caggctcccc agcaggcaga
     4921 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     4981 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     5041 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     5101 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     5161 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     5221 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg
     5281 gcatagtata atacgacaag gtgaggaact aaaccatggc caagcctttg tctcaagaag
     5341 aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact
     5401 acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat
     5461 atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg
     5521 cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc
     5581 cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga
     5641 aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg
     5701 tgtgggaggg ctaagcacaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca
     5761 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     5821 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     5881 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     5941 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     6001 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     6061 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     6121 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6181 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     6241 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     6301 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     6361 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     6421 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     6481 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     6541 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     6601 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     6661 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     6721 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6781 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     6841 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     6901 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     6961 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     7021 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     7081 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     7141 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     7201 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     7261 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     7321 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     7381 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     7441 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     7501 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     7561 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     7621 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     7681 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     7741 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     7801 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     7861 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     7921 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     7981 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8041 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     8101 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     8161 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     8221 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     8281 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     8341 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     8401 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     8461 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     8521 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     8581 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8641 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     8701 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     8761 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8821 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     8881 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     8941 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     9001 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatg
//
