LOCUS       V011777                 8813 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011777
VERSION     V011777
KEYWORDS    pLenti6.2 V5-DEST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8813)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8813)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     enhancer        1816..2119
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2120..2323
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    2440..2564
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2589..2619
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2673..3329
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             3674..3976
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(4020..4144)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4197..4238
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     promoter        4271..4769
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     promoter        4783..4830
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             4849..5244
                     /label="BSD"
                     /note="blasticidin S deaminase"
     LTR             5334..5567
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5639..5773
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5800..5935
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(5956..5974)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5984..6000)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6142..6597
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6623..6727
                     /label="AmpR promoter"
     CDS             6728..7585
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7759..8347
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8635..8656
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8671..8701
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8709..8725
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8733..8749
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        8770..8788
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata
     1801 agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1861 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     1921 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1981 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2041 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2101 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     2161 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     2221 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2281 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     2341 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gactctagag
     2401 gatccactag tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagct
     2461 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
     2521 cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca
     2581 ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc
     2641 cggcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
     2701 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
     2761 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
     2821 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
     2881 atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
     2941 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc
     3001 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa
     3061 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct
     3121 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg
     3181 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc
     3241 aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac
     3301 agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta ctaaaagcca
     3361 gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt
     3421 atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag tgacagttga
     3481 cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc
     3541 acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag
     3601 gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac
     3661 agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
     3721 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct
     3781 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
     3841 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat
     3901 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
     3961 gatgttctgg ggaatataaa tgtcaggctc cgttatacac agccagtctg caggtcgacc
     4021 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
     4081 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag
     4141 tggttgatat ccagcacagt ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta
     4201 agcctatccc taaccctctc ctcggtctcg attctacgcg taccggttag taatgacgat
     4261 ggtacctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
     4321 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
     4381 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
     4441 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
     4501 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcdg
     4561 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
     4621 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
     4681 cgaagtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
     4741 tctcctcttc ctcatctccg ggcctttcga ctctagacac gtgttgacaa ttaatcatcg
     4801 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagcct
     4861 ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc
     4921 atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact
     4981 ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact
     5041 gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg
     5101 ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc
     5161 aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg
     5221 ccctctggtt atgtgtggga gggctaagca caattcgagc tcggtacctt taagaccaat
     5281 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     5341 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     5401 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     5461 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     5521 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     5581 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     5641 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5701 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     5761 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     5821 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     5881 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     5941 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     6001 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     6061 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     6121 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     6181 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     6241 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     6301 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     6361 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     6421 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     6481 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     6541 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     6601 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     6661 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     6721 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     6781 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     6841 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     6901 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     6961 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     7021 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     7081 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     7141 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     7201 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     7261 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     7321 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     7381 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     7441 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     7501 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     7561 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     7621 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     7681 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7741 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7801 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7861 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     7921 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     7981 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8041 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8101 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8161 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8221 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8281 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8341 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8401 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     8461 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     8521 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     8581 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     8641 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     8701 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     8761 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag ctt
//
