LOCUS       V011778                 8956 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011778
VERSION     V011778
KEYWORDS    pLenti6.2 N-Lumio V5-DEST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8956)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8956)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     enhancer        1882..2185
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2186..2389
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        2434..2452
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2483..2485
                     /codon_start=1
                     /product="start codon"
                     /label="start codon"
                     /note="ATG"
                     /translation="M"
     CDS             2495..2512
                     /label="tetracysteine tag"
                     /note="tetracysteine peptide that binds biarsenical
                     labeling reagents"
     CDS             2528..2569
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     protein_bind    2582..2706
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2731..2761
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2815..3471
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             3816..4118
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(4162..4286)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4339..4380
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     promoter        4413..4912
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     promoter        4926..4973
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             4992..5387
                     /label="BSD"
                     /note="blasticidin S deaminase"
     LTR             5477..5710
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5782..5916
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5943..6078
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(6099..6117)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6127..6143)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6285..6740
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6766..6870
                     /label="AmpR promoter"
     CDS             6871..7728
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7902..8490
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8778..8799
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8814..8844
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8852..8868
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8876..8892
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        8913..8931
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgatg
     1801 tcgacgttaa cgctagcgac tgggtctgcg atgtcgacgt taacgctagc gactgggtct
     1861 gcgataagct tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     1921 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     1981 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     2041 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     2101 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     2161 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     2221 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     2281 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     2341 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac
     2401 tagagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca
     2461 agctggctag ttaagctgca ccatggctgg tggctgttgt cctggctgtt gcggtggcgg
     2521 caagctgggt aagcctatcc ctaaccctct cctcggtctc gattctacga gtgctgttat
     2581 cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt
     2641 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
     2701 actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat
     2761 gtgtggattt tgagttagga tccggcgaga ttttcaggag ctaaggaagc taaaatggag
     2821 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
     2881 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
     2941 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
     3001 cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg
     3061 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
     3121 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
     3181 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg
     3241 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
     3301 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag
     3361 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
     3421 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaaagatct
     3481 ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt
     3541 ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc
     3601 agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt
     3661 caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga
     3721 acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa
     3781 cggctctttt gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata
     3841 aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg
     3901 ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg
     3961 aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg
     4021 ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg
     4081 acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac
     4141 acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta
     4201 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct
     4261 cgttcagctt tcttgtacaa agtggttgat atccagcaca gtggcggccg ctcgagtcta
     4321 gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg
     4381 cgtaccggtt agtaatgacg atggtaccta ccgggtaggg gaggcgcttt tcccaaggca
     4441 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
     4501 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
     4561 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
     4621 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
     4681 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     4741 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
     4801 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     4861 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgactctaga
     4921 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     4981 ggaactaaac catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa
     5041 cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct
     5101 ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg
     5161 cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg
     5221 tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc
     5281 ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg
     5341 cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gcacaattcg
     5401 agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     5461 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     5521 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     5581 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     5641 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     5701 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     5761 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     5821 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     5881 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     5941 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     6001 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     6061 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     6121 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     6181 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     6241 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     6301 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     6361 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     6421 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     6481 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     6541 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     6601 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     6661 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     6721 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     6781 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     6841 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     6901 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     6961 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     7021 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     7081 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     7141 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     7201 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     7261 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     7321 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     7381 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     7441 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     7501 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     7561 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     7621 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     7681 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     7741 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     7801 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     7861 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7921 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7981 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     8041 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     8101 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     8161 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     8221 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     8281 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     8341 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     8401 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     8461 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     8521 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     8581 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     8641 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     8701 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     8761 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     8821 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     8881 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     8941 ctggagctgc aagctt
//
