LOCUS       V011779                 8835 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011779
VERSION     V011779
KEYWORDS    pLenti6.2 C-Lumio V5-DEST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8835)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8835)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     enhancer        1816..2119
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2120..2323
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    2440..2564
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2589..2619
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2673..3329
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             3674..3976
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(4020..4144)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4158..4199
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     CDS             4218..4235
                     /label="tetracysteine tag"
                     /note="tetracysteine peptide that binds biarsenical
                     labeling reagents"
     promoter        4292..4791
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     promoter        4805..4852
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             4871..5266
                     /label="BSD"
                     /note="blasticidin S deaminase"
     LTR             5356..5589
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5661..5795
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5822..5957
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(5978..5996)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6006..6022)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6164..6619
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6645..6749
                     /label="AmpR promoter"
     CDS             6750..7607
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7781..8369
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8657..8678
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8693..8723
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8731..8747
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8755..8771
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        8792..8810
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata
     1801 agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1861 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     1921 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1981 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2041 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2101 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     2161 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     2221 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2281 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     2341 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gactctagag
     2401 gatccactag tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagct
     2461 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
     2521 cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca
     2581 ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc
     2641 cggcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
     2701 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
     2761 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
     2821 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
     2881 atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
     2941 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc
     3001 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa
     3061 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct
     3121 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg
     3181 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc
     3241 aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac
     3301 agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta ctaaaagcca
     3361 gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt
     3421 atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag tgacagttga
     3481 cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc
     3541 acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag
     3601 gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac
     3661 agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
     3721 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct
     3781 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
     3841 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat
     3901 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
     3961 gatgttctgg ggaatataaa tgtcaggctc cgttatacac agccagtctg caggtcgacc
     4021 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
     4081 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag
     4141 tggttgatgc tgttaacggg aagcctatcc ctaaccctct cctcggtctc gattctacgc
     4201 gtaccggtgc tggtggctgt tgtcctggct gttgcggtgg cggctagtaa tgagtttcgc
     4261 gtaccggtta gtaatgacga tggtacctac cgggtagggg aggcgctttt cccaaggcag
     4321 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
     4381 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
     4441 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
     4501 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
     4561 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
     4621 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
     4681 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
     4741 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gactctagac
     4801 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     4861 gaactaaacc atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac
     4921 ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc
     4981 tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc
     5041 agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt
     5101 cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct
     5161 tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc
     5221 agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cacaattcga
     5281 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     5341 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     5401 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     5461 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     5521 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     5581 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     5641 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     5701 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5761 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     5821 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     5881 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     5941 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     6001 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     6061 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     6121 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     6181 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     6241 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     6301 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     6361 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     6421 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     6481 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     6541 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     6601 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6661 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6721 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6781 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     6841 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6901 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6961 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     7021 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     7081 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7141 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     7201 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     7261 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     7321 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     7381 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     7441 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     7501 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7561 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7621 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7681 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7741 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7801 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7861 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7921 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7981 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8041 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8101 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8161 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8221 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8281 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8341 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     8401 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     8461 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     8521 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     8581 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     8641 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     8701 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     8761 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     8821 tggagctgca agctt
//
