LOCUS       V011782                 9087 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011782
VERSION     V011782
KEYWORDS    pLenti6 capTEV-NT-DEST1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9087)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9087)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     protein_bind    1823..1843
                     /label="attB4"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     enhancer        1872..2251
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2252..2455
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2510..2812
                     /label="capTEV(TM)"
                     /note="composite tag for in vivo biotinylation, TEV
                     protease cleavage, and 6xHis tagging"
     protein_bind    2840..2964
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2989..3019
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             3073..3729
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             4074..4376
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(4420..4544)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4597..4638
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     promoter        4680..5009
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        5057..5104
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             5123..5518
                     /label="BSD"
                     /note="blasticidin S deaminase"
     LTR             5608..5841
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5913..6047
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6074..6209
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(6230..6248)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6258..6274)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6416..6871
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6897..7001
                     /label="AmpR promoter"
     CDS             7002..7859
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8033..8621
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8909..8930
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8945..8975
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8983..8999
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9007..9023
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        9044..9062
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgatg
     1801 tcgacgttaa cgctagtgat atcaactttg tatagaaaag ttgaacgatg tacgggccag
     1861 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     1921 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     1981 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     2041 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     2101 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     2161 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     2221 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     2281 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     2341 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     2401 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg
     2461 gctaactaga gaacccactg cttactggct tatcgaaatt agcttcacca tgggcgccgg
     2521 caccccggtg accgccccgc tggcgggcac tatctggaag gtgctggcca gcgaaggcca
     2581 gacggtggcc gcaggcgagg tgctgctgat tctggaagcc atgaagatgg aaaccgaaat
     2641 ccgcgccgcg caggccggga ccgtgcgcgg tatcgcggtg aaagccggcg acgcggtggc
     2701 ggtcggcgac accctgatga ccctggcggg ctctggatcc gagaatcttt attttcaggg
     2761 tcaattggag aatctttatt ttcagggtca attgcatcat catcatcatc atggtgaagg
     2821 ccgaattctg cagatatcaa caagtttgta caaaaaagct gaacgagaaa cgtaaaatga
     2881 tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa
     2941 aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt tacactttat
     3001 gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct
     3061 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
     3121 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
     3181 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
     3241 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
     3301 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     3361 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
     3421 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     3481 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
     3541 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     3601 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
     3661 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     3721 ggcggggcgt aaagatctgg atccggctta ctaaaagcca gataacagta tgcgtatttg
     3781 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa
     3841 agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc
     3901 tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc
     3961 ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc
     4021 ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt
     4081 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
     4141 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
     4201 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
     4261 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
     4321 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
     4381 tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt
     4441 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
     4501 atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttgatat ccagcacagt
     4561 ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc
     4621 ctcggtctcg attctacgcg taccggttag taatgagttt ggaattaatt ctgtggaatg
     4681 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4741 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4801 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     4861 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     4921 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     4981 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     5041 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     5101 cgacaaggtg aggaactaaa ccatggccaa gcctttgtct caagaagaat ccaccctcat
     5161 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag
     5221 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg
     5281 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct
     5341 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg
     5401 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg
     5461 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta
     5521 agcacaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta
     5581 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     5641 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     5701 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     5761 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     5821 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     5881 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     5941 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     6001 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     6061 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     6121 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     6181 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     6241 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     6301 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     6361 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     6421 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     6481 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     6541 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     6601 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     6661 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     6721 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     6781 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     6841 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     6901 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6961 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     7021 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     7081 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     7141 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     7201 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     7261 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     7321 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     7381 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     7441 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     7501 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     7561 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     7621 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     7681 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     7741 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     7801 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     7861 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     7921 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     7981 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     8041 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     8101 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     8161 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     8221 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     8281 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     8341 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     8401 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     8461 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     8521 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     8581 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     8641 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     8701 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     8761 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     8821 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     8881 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     8941 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     9001 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     9061 gggaacaaaa gctggagctg caagctt
//
