LOCUS       V011783                 8991 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011783
VERSION     V011783
KEYWORDS    pLenti6 capTEV-CT-DEST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8991)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8991)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     enhancer        1816..2119
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2120..2323
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    2440..2564
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2589..2619
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2673..3329
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             3674..3976
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(4020..4144)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4200..4484
                     /label="capTEV(TM)"
                     /note="composite tag for in vivo biotinylation, TEV
                     protease cleavage, and 6xHis tagging"
     CDS             4500..4541
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     promoter        4583..4912
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        4960..5007
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             5026..5421
                     /label="BSD"
                     /note="blasticidin S deaminase"
     LTR             5511..5744
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5816..5951
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5978..6113
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(6134..6152)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6162..6178)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6320..6775
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6801..6905
                     /label="AmpR promoter"
     CDS             6906..7763
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7937..8525
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8813..8834
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8849..8879
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8887..8903
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8911..8927
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        8948..8966
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata
     1801 agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1861 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     1921 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1981 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2041 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2101 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     2161 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     2221 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2281 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     2341 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gactctagag
     2401 gatccactag tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagct
     2461 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
     2521 cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca
     2581 ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc
     2641 cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
     2701 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
     2761 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
     2821 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
     2881 atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
     2941 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc
     3001 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa
     3061 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct
     3121 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg
     3181 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc
     3241 aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac
     3301 agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta ctaaaagcca
     3361 gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt
     3421 atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga
     3481 cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc
     3541 acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag
     3601 gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac
     3661 aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
     3721 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct
     3781 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
     3841 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat
     3901 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
     3961 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc
     4021 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
     4081 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag
     4141 tggttgatat ccagcacagt ggcggccgct cgagtctaga gggcccgcgg ttcgaattcc
     4201 atcatcatca tcatcatggt gagaatcttt attttcaggg tcaattggag aatctttatt
     4261 ttcagggtgg cgccggcacc ccggtgaccg ccccgctggc gggcactatc tggaaggtgc
     4321 tggccagcga aggccagacg gtggccgcag gcgaggtgct gctgattctg gaagccatga
     4381 agatggaaac cgaaatccgc gccgcgcagg ccgggaccgt gcgcggtatc gcggtgaaag
     4441 ccggcgacgc ggtggcggtc ggcgacaccc tgatgaccct ggcgggctct ggatccgaag
     4501 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt tagtaatgag
     4561 tttggaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     4621 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     4681 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     4741 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     4801 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     4861 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     4921 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     4981 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagcctttg
     5041 tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc
     5101 tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt
     5161 gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct
     5221 gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc
     5281 atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa
     5341 gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc
     5401 tctggttatg tgtgggaggg ctaagcacaa ttcgagctcg gtacctttaa gaccaatgac
     5461 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     5521 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     5581 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     5641 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     5701 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     5761 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     5821 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5881 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     5941 tagtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     6001 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     6061 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     6121 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     6181 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     6241 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     6301 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6361 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     6421 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     6481 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     6541 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6601 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6661 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     6721 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     6781 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     6841 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     6901 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     6961 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     7021 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     7081 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     7141 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     7201 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     7261 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     7321 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     7381 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     7441 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     7501 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     7561 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     7621 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     7681 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     7741 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     7801 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     7861 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7921 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7981 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8041 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     8101 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8161 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8221 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8281 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     8341 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8401 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8461 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8521 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     8581 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     8641 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     8701 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     8761 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     8821 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     8881 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     8941 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct t
//
