LOCUS       V011780                 8687 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011780
VERSION     V011780
KEYWORDS    pLenti6 V5-DEST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8687)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8687)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     enhancer        1816..2119
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2120..2323
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    2440..2564
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2589..2619
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2673..3329
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             3674..3976
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(4020..4144)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4197..4238
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     promoter        4280..4609
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        4657..4704
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             4723..5118
                     /label="BSD"
                     /note="blasticidin S deaminase"
     LTR             5208..5441
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5513..5647
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5674..5809
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(5830..5848)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5858..5874)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6016..6471
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6497..6601
                     /label="AmpR promoter"
     CDS             6602..7459
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7633..8221
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8509..8530
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8545..8575
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8583..8599
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8607..8623
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        8644..8662
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata
     1801 agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1861 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     1921 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1981 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2041 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2101 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     2161 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     2221 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2281 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     2341 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gactctagag
     2401 gatccactag tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagct
     2461 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
     2521 cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca
     2581 ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc
     2641 cggcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
     2701 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
     2761 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
     2821 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
     2881 atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
     2941 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc
     3001 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa
     3061 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct
     3121 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg
     3181 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc
     3241 aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac
     3301 agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta ctaaaagcca
     3361 gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt
     3421 atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag tgacagttga
     3481 cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc
     3541 acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag
     3601 gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac
     3661 agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
     3721 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct
     3781 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
     3841 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat
     3901 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
     3961 gatgttctgg ggaatataaa tgtcaggctc cgttatacac agccagtctg caggtcgacc
     4021 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
     4081 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag
     4141 tggttgatat ccagcacagt ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta
     4201 agcctatccc taaccctctc ctcggtctcg attctacgcg taccggttag taatgagttt
     4261 ggaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     4321 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     4381 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     4441 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     4501 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     4561 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     4621 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     4681 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gcctttgtct
     4741 caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct
     4801 gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc
     4861 aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct
     4921 gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc
     4981 ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc
     5041 atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct
     5101 ggttatgtgt gggagggcta agcacaattc gagctcggta cctttaagac caatgactta
     5161 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     5221 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     5281 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     5341 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     5401 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     5461 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     5521 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     5581 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     5641 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     5701 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5761 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     5821 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     5881 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     5941 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     6001 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6061 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6121 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6181 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6241 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     6301 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     6361 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     6421 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     6481 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6541 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6601 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     6661 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     6721 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     6781 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     6841 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     6901 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     6961 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     7021 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     7081 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7141 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     7201 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     7261 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     7321 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     7381 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7441 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     7501 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     7561 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7621 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7681 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7741 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     7801 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7861 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7921 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7981 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8041 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8101 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8161 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8221 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8281 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8341 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     8401 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     8461 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     8521 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     8581 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     8641 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagctt
//
