LOCUS       V001643                 9570 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V001643
VERSION     V001643
KEYWORDS    pLenti6/UbC/mSlc7a1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9570)
  AUTHORS   Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K,
            Yamanaka S
  TITLE     Induction of pluripotent stem cells from adult human fibroblasts by
            defined factors.
  JOURNAL   Cell. 2007 Nov 30. 131(5):861-72.
   PUBMED   18035408
REFERENCE   2  (bases 1 to 9570)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9570)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007
            Nov 30. 131(5):861-72."
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     promoter        1803..3014
                     /label="UbC promoter"
                     /note="human ubiquitin C promoter"
     protein_bind    3074..3098
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3119..4984
                     /gene="Slc7a1"
                     /label="High affinity cationic amino acid transporter 1"
                     /note="High affinity cationic amino acid transporter 1 from
                     Mus musculus. Accession#: Q09143"
     protein_bind    complement(5003..5027)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5080..5121
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     promoter        5163..5492
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        5540..5587
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             5606..6001
                     /label="BSD"
                     /note="blasticidin S deaminase"
     LTR             6091..6324
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6396..6530
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6557..6692
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(6713..6731)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6741..6757)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6899..7354
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7380..7484
                     /label="AmpR promoter"
     CDS             7485..8342
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8516..9104
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     9258..9275
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     protein_bind    9392..9413
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9428..9458
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9466..9482
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9490..9506
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        9527..9545
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggat
     1801 ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc
     1861 tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac
     1921 agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt
     1981 aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga
     2041 ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg
     2101 atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg
     2161 gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg
     2221 ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc
     2281 gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag
     2341 cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt
     2401 gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc
     2461 ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc
     2521 ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca
     2581 gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg
     2641 tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg
     2701 tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga
     2761 atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt
     2821 cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg
     2881 ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt
     2941 caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg
     3001 cttttttgtt agacgaagct tggtaccgag ctcggatcca ctagtccagt gtggtggaat
     3061 tctgcagata tcaacaagtt tgtacaaaaa agcaggctcc gcggccgccc ccttcaccat
     3121 gggctgcaaa aacctgctcg gtctgggcca gcagatgctg cgccggaagg tggtggactg
     3181 cagccgggag gagagccggc tgtcccgctg cctcaacacc tatgacctgg tagctcttgg
     3241 ggtgggcagc accttgggcg ctggtgtcta tgtcctagcc ggtgccgtgg cccgtgaaaa
     3301 tgctggccct gccatcgtca tctccttctt gattgctgct ctcgcctccg tgctggccgg
     3361 cctgtgctac ggcgagtttg gtgcccgtgt ccccaagacg ggctcagcct acctctacag
     3421 ctacgtgacg gtgggggagc tttgggcctt catcactggc tggaacctga ttctctccta
     3481 catcatcggt acttcaagcg tggcaagagc ctggagtgcg acttttgacg agctgatagg
     3541 caagcccatc ggagagttct cacgtcagca catggccctg aatgctcctg gggtgctggc
     3601 ccaaaccccg gacatatttg ctgtgattat aattatcatc ttaacaggac tgttaactct
     3661 tggcgtgaag gagtcagcca tggtcaacaa aattttcacc tgtatcaatg tcctggtctt
     3721 gtgcttcatc gtggtgtccg ggttcgtgaa aggctccatt aaaaactggc agctcacgga
     3781 gaaaaatttc tcctgtaaca acaacgacac aaacgtgaaa tacggtgagg gagggtttat
     3841 gccctttgga ttctctggtg tcctgtcagg ggcagcgacc tgcttttatg ccttcgtggg
     3901 ctttgactgc atcgccacca caggggaaga agtcaagaac ccccagaagg ccattcctgt
     3961 gggcatcgtg gcgtccctcc tcatttgctt catagcgtac tttggcgtgt ccgccgctct
     4021 cacgctcatg atgccttact tctgcctgga catcgacagc ccgctgcctg gtgccttcaa
     4081 gcaccagggc tgggaagaag ctaagtacgc agtggccatt ggctctctct gcgcactttc
     4141 caccagtctc ctaggctcca tgtttcccat gccccgagtt atctatgcca tggctgaaga
     4201 tggactactg tttaaatttt tggccaaaat caacaatagg accaaaacac ccgtaatcgc
     4261 cactgtgacc tcaggcgcca ttgctgctgt gatggccttc ctctttgaac tgaaggacct
     4321 ggtggacctc atgtccattg gcactctcct ggcttactct ttggtggctg cctgtgtttt
     4381 ggtcttacgg taccagccag aacaacctaa tctggtatac cagatggcca gaaccaccga
     4441 ggagctagat cgagtagatc agaatgagct ggtcagtgcc agtgaatcac agacaggctt
     4501 tttaccggta gccgagaagt tttctctgaa atccatcctc tcacccaaga acgtggagcc
     4561 ctccaaattc tcagggctaa ttgtgaacat ttcagccggc ctcctagccg ctcttatcat
     4621 caccgtgtgc attgtggccg tgcttggaag agaggccctg gccgaaggga cactgtgggc
     4681 agtctttgta atgacagggt cagtcctcct ctgcatgctg gtgacaggca tcatctggag
     4741 acagcctgag agcaagacca agctctcatt taaggtaccc tttgtccccg tacttcctgt
     4801 cttgagcatc ttcgtgaaca tctatctcat gatgcagctg gaccagggca cgtgggtccg
     4861 gtttgcagtg tggatgctga taggtttcac catctatttc ggttatggga tctggcacag
     4921 tgaggaagcg tccctggctg ctggccaggc aaagactcct gacagcaact tggaccagtg
     4981 caaataaagg gtgggcgcgc cgacccagct ttcttgtaca aagtggttga tatccagcac
     5041 agtggcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat ccctaaccct
     5101 ctcctcggtc tcgattctac gcgtaccggt tagtaatgag tttggaatta attctgtgga
     5161 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     5221 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     5281 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     5341 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     5401 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     5461 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     5521 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
     5581 atacgacaag gtgaggaact aaaccatggc caagcctttg tctcaagaag aatccaccct
     5641 cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc
     5701 cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac
     5761 tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa
     5821 cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg
     5881 gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga
     5941 tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg
     6001 ctaagcacaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     6061 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     6121 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     6181 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6241 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6301 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     6361 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     6421 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     6481 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     6541 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6601 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     6661 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     6721 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     6781 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     6841 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     6901 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     6961 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     7021 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     7081 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     7141 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     7201 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     7261 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     7321 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     7381 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     7441 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     7501 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     7561 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     7621 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     7681 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     7741 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     7801 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     7861 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     7921 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     7981 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     8041 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     8101 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     8161 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     8221 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     8281 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     8341 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     8401 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     8461 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     8521 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     8581 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     8641 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     8701 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     8761 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8821 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8881 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     8941 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     9001 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     9061 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     9121 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     9181 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     9241 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     9301 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     9361 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     9421 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     9481 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     9541 aaagggaaca aaagctggag ctgcaagctt
//
