LOCUS       V011372                10073 bp    DNA     circular SYN 13-JAN-2022
DEFINITION  Exported.
ACCESSION   V011372
VERSION     V011372
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10073)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 10073)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(811..835)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             888..929
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     promoter        971..1300
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        1312..1359
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             1378..1749
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     LTR             1845..2078
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2150..2284
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      2311..2446
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(2467..2485)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2653..3108
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3134..3238
                     /label="AmpR promoter"
     CDS             3239..4096
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4270..4858
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    5146..5167
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5182..5212
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     promoter        5281..5299
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5327..5553
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    5781..5906
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    6399..6632
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             6817..6861
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             7010..7051
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     enhancer        7140..7443
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7444..7647
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    7764..7788
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(7820..10073,1..791)
                     /label="lacZ"
                     /note="beta-galactosidase"
ORIGIN
        1 gccagtcagg ctttctttca cagatgtgga ttggcgataa aaaacaactg ctgacgccgc
       61 tgcgcgatca gttcacccgt gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc
      121 gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc gggccattac caggccgaag
      181 cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc ggtgctgatt acgaccgctc
      241 acgcgtggca gcatcagggg aaaaccttat ttatcagccg gaaaacctac cggattgatg
      301 gtagtggtca aatggcgatt accgttgatg ttgaagtggc gagcgataca ccgcatccgg
      361 cgcggattgg cctgaactgc cagctggcgc aggtagcaga gcgggtaaac tggctcggat
      421 tagggccgca agaaaactat cccgaccgcc ttactgccgc ctgttttgac cgctgggatc
      481 tgccattgtc agacatgtat accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg
      541 ggacgcgcga attgaattat ggcccacacc agtggcgcgg cgacttccag ttcaacatca
      601 gccgctacag tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg cacgcggaag
      661 aaggcacatg gctgaatatc gacggtttcc atatggggat tggtggcgac gactcctgga
      721 gcccgtcagt atcggcggag ttccagctga gcgccggtcg ctaccattac cagttggtct
      781 ggtgtcaaaa aactaagggt gggcgcgccg acccagcttt cttgtacaaa gtggttgata
      841 tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc
      901 ctaaccctct cctcggtctc gattctacgc gtaccggtta gtaatgagtt tggaattaat
      961 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     1021 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     1081 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     1141 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     1201 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     1261 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccc tgttgacaat
     1321 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     1381 gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag
     1441 ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg
     1501 gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac
     1561 accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc
     1621 gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg
     1681 tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag
     1741 gagcaggact gacacgtgct acgagattta aatggtacct ttaagaccaa tgacttacaa
     1801 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     1861 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     1921 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     1981 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     2041 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     2101 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     2161 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     2221 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     2281 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     2341 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     2401 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     2461 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     2521 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     2581 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     2641 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     2701 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     2761 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     2821 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     2881 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     2941 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     3001 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     3061 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     3121 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     3181 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     3241 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     3301 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     3361 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     3421 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     3481 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     3541 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     3601 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     3661 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     3721 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     3781 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     3841 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     3901 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     3961 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     4021 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     4081 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     4141 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     4201 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4261 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4321 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4381 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4441 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4501 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4561 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4621 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4681 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4741 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4801 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4861 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     4921 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     4981 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     5041 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     5101 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     5161 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     5221 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     5281 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
     5341 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
     5401 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
     5461 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
     5521 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
     5581 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     5641 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     5701 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     5761 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     5821 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     5881 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     5941 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     6001 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     6061 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     6121 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     6181 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     6241 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     6301 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     6361 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     6421 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     6481 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     6541 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     6601 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     6661 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     6721 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     6781 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     6841 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     6901 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     6961 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     7021 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     7081 gacagatcca ttcgattagt gaacggatct cgacggtatc gataagcttg ggagttccgc
     7141 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     7201 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     7261 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     7321 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     7381 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     7441 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     7501 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     7561 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     7621 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
     7681 catccacgct gttttgacct ccatagaaga caccgactct agaggatcca ctagtccagt
     7741 gtggtggaat tctgcagata tcaacaagtt tgtacaaaaa agcaggctcc gcggccgccc
     7801 cttcaccatg atagatcccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     7861 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     7921 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg
     7981 gtttccggca ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga
     8041 tactgtcgtc gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa
     8101 cgtaacctat cccattacgg tcaatccgcc gtttgttccc acggagaatc cgacgggttg
     8161 ttactcgctc acatttaatg ttgatgaaag ctggctacag gaaggccaga cgcgaattat
     8221 ttttgatggc gttaactcgg cgtttcatct gtggtgcaac gggcgctggg tcggttacgg
     8281 ccaggacagt cgtttgccgt ctgaatttga cctgagcgca tttttacgcg ccggagaaaa
     8341 ccgcctcgcg gtgatggtgc tgcgttggag tgacggcagt tatctggaag atcaggatat
     8401 gtggcggatg agcggcattt tccgtgacgt ctcgttgctg cataaaccga ctacacaaat
     8461 cagcgatttc catgttgcca ctcgctttaa tgatgatttc agccgcgctg tactggaggc
     8521 tgaagttcag atgtgcggcg agttgcgtga ctacctacgg gtaacagttt ctttatggca
     8581 gggtgaaacg caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg
     8641 tggtggttat gccgatcgcg tcacactacg tctgaacgtc gaaaacccga aactgtggag
     8701 cgccgaaatc ccgaatctct atcgtgcggt ggttgaactg cacaccgccg acggcacgct
     8761 gattgaagca gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa atggtctgct
     8821 gctgctgaac ggcaagccgt tgctgattcg aggcgttaac cgtcacgagc atcatcctct
     8881 gcatggtcag gtcatggatg agcagacgat ggtgcaggat atcctgctga tgaagcagaa
     8941 caactttaac gccgtgcgct gttcgcatta tccgaaccat ccgctgtggt acacgctgtg
     9001 cgaccgctac ggcctgtatg tggtggatga agccaatatt gaaacccacg gcatggtgcc
     9061 aatgaatcgt ctgaccgatg atccgcgctg gctaccggcg atgagcgaac gcgtaacgcg
     9121 aatggtgcag cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg ggaatgaatc
     9181 aggccacggc gctaatcacg acgcgctgta tcgctggatc aaatctgtcg atccttcccg
     9241 cccggtgcag tatgaaggcg gcggagccga caccacggcc accgatatta tttgcccgat
     9301 gtacgcgcgc gtggatgaag accagccctt cccggctgtg ccgaaatggt ccatcaaaaa
     9361 atggctttcg ctacctggag agacgcgccc gctgatcctt tgcgaatacg cccacgcgat
     9421 gggtaacagt cttggcggtt tcgctaaata ctggcaggcg tttcgtcagt atccccgttt
     9481 acagggcggc ttcgtctggg actgggtgga tcagtcgctg attaaatatg atgaaaacgg
     9541 caacccgtgg tcggcttacg gcggtgattt tggcgatacg ccgaacgatc gccagttctg
     9601 tatgaacggt ctggtctttg ccgaccgcac gccgcatcca gcgctgacgg aagcaaaaca
     9661 ccagcagcag tttttccagt tccgtttatc cgggcaaacc atcgaagtga ccagcgaata
     9721 cctgttccgt catagcgata acgagctcct gcactggatg gtggcgctgg atggtaagcc
     9781 gctggcaagc ggtgaagtgc ctctggatgt cgctccacaa ggtaaacagt tgattgaact
     9841 gcctgaacta ccgcagccgg agagcgccgg gcaactctgg ctcacagtac gcgtagtgca
     9901 accgaacgcg accgcatggt cagaagccgg gcacatcagc gcctggcagc agtggcgtct
     9961 ggcggaaaac ctcagtgtga cgctccccgc cgcgtcccac gccatcccgc atctgaccac
    10021 cagcgaaatg gatttttgca tcgagctggg taataagcgt tggcaattta acc
//
