LOCUS       V011785                 8634 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011785
VERSION     V011785
KEYWORDS    pLenti4 V5-DEST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8634)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     enhancer        1816..2119
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2120..2323
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    2440..2564
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2589..2619
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2673..3329
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             3674..3976
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(4020..4144)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4197..4238
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     promoter        4280..4610
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        4622..4669
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             4688..5059
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     LTR             5155..5388
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5460..5594
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5621..5756
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(5777..5795)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5805..5821)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      5963..6418
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6444..6548
                     /label="AmpR promoter"
     CDS             6549..7406
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7580..8168
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8456..8477
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8492..8522
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8530..8546
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8554..8570
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        8591..8609
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata
     1801 agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1861 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     1921 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1981 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2041 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2101 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     2161 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     2221 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2281 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     2341 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gactctagag
     2401 gatccactag tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagct
     2461 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
     2521 cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca
     2581 ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc
     2641 cggcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
     2701 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
     2761 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
     2821 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
     2881 atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
     2941 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc
     3001 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa
     3061 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct
     3121 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg
     3181 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc
     3241 aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac
     3301 agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta ctaaaagcca
     3361 gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt
     3421 atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag tgacagttga
     3481 cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc
     3541 acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag
     3601 gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac
     3661 agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
     3721 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct
     3781 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
     3841 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat
     3901 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
     3961 gatgttctgg ggaatataaa tgtcaggctc cgttatacac agccagtctg caggtcgacc
     4021 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
     4081 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag
     4141 tggttgatat ccagcacagt ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta
     4201 agcctatccc taaccctctc ctcggtctcg attctacgcg taccggttag taatgagttt
     4261 ggaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagg
     4321 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     4381 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     4441 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     4501 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     4561 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccc
     4621 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     4681 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     4741 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     4801 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     4861 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     4921 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     4981 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     5041 cgtggccgag gagcaggact gacacgtgct acgagattta aatggtacct ttaagaccaa
     5101 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     5161 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     5221 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     5281 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     5341 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     5401 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     5461 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     5521 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     5581 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     5641 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     5701 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5761 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     5821 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     5881 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     5941 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     6001 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     6061 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     6121 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     6181 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     6241 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     6301 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     6361 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     6421 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     6481 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     6541 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     6601 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     6661 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     6721 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     6781 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     6841 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     6901 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6961 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     7021 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     7081 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     7141 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     7201 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     7261 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     7321 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     7381 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     7441 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7501 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7561 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7621 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7681 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     7741 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7801 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7861 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7921 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7981 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8041 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8101 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8161 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     8221 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     8281 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     8341 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     8401 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     8461 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     8521 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     8581 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gctt
//
