LOCUS       V001635                 8598 bp    DNA     circular SYN 13-JAN-2022
DEFINITION  Exported.
ACCESSION   V001635
VERSION     V001635
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8598)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8598)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     enhancer        1816..2119
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2120..2323
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    2325..2343
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2346..2364
                     /gene="tetO"
                     /label="tetracycline repressor TetR binding site"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2405..2529
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2554..2584
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2638..3294
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             3639..3941
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(3985..4109)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4162..4203
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     promoter        4245..4574
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        4586..4633
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             4652..5023
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     LTR             5119..5352
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5424..5558
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5585..5720
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(5741..5759)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5769..5785)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      5927..6382
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6408..6512
                     /label="AmpR promoter"
     CDS             6513..7370
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7544..8132
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8420..8441
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8456..8486
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8494..8510
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8518..8534
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        8555..8573
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata
     1801 agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1861 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     1921 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1981 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2041 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2101 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     2161 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     2221 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2281 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag
     2341 agatctccct atcagtgata gagatcgtcg actagtccag tgtggtggaa ttctgcagat
     2401 atcaacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat
     2461 attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca
     2521 gtcactatgg cggccgcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2581 aatgtgtgga ttttgagtta ggatccggcg agattttcag gagctaagga agctaaaatg
     2641 gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat
     2701 tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt
     2761 acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac
     2821 attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag
     2881 ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg
     2941 ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca catatattcg
     3001 caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat
     3061 atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc
     3121 aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac
     3181 aaggtgctga tgccgctggc gattcaggtt catcatgccg tctgtgatgg cttccatgtc
     3241 ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaaaga
     3301 tctggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc
     3361 ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg tgtgctatga
     3421 agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga
     3481 tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc
     3541 cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat
     3601 gaacggctct tttgctgacg agaacaggga ctggtgaaat gcagtttaag gtttacacct
     3661 ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc
     3721 ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc
     3781 gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata
     3841 tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa
     3901 atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccgtta
     3961 tacacagcca gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagtattat
     4021 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
     4081 tctcgttcag ctttcttgta caaagtggtt gatatccagc acagtggcgg ccgctcgagt
     4141 ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct
     4201 acgcgtaccg gttagtaatg agtttggaat taattctgtg gaatgtgtgt cagttagggt
     4261 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4321 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4381 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     4441 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     4501 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     4561 taggcttttg caaaaagctc cccctgttga caattaatca tcggcatagt atatcggcat
     4621 agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt
     4681 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
     4741 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
     4801 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
     4861 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
     4921 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
     4981 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga
     5041 tttaaatggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     5101 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     5161 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     5221 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     5281 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     5341 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     5401 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     5461 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     5521 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     5581 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     5641 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     5701 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     5761 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     5821 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     5881 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     5941 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     6001 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     6061 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     6121 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     6181 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     6241 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     6301 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     6361 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6421 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     6481 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     6541 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     6601 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     6661 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     6721 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     6781 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     6841 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     6901 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     6961 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7021 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7081 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7141 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7201 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     7261 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7321 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7381 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     7441 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     7501 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     7561 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     7621 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     7681 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     7741 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     7801 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     7861 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     7921 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     7981 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8041 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8101 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8161 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     8221 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     8281 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     8341 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     8401 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     8461 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     8521 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     8581 agctggagct gcaagctt
//
