LOCUS       V011786                 8054 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011786
VERSION     V011786
KEYWORDS    pLenti4 BLOCK-iT-DEST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8054)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8054)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     protein_bind    1806..1826
                     /label="attB4"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     protein_bind    1861..1985
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2010..2040
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2094..2750
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             3095..3397
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(3441..3565)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3618..3659
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     promoter        3701..4030
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        4042..4089
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             4108..4479
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     LTR             4575..4808
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4880..5014
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5041..5176
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(5197..5215)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5225..5241)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      5383..5838
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5864..5968
                     /label="AmpR promoter"
     CDS             5969..6826
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7000..7588
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    7876..7897
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7912..7942
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7950..7966
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7974..7990
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        8011..8029
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcgctcaact ttgtatagaa aagttgacta gtccagtgtg gtggaattct gcagatatca
     1861 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
     1921 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
     1981 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
     2041 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
     2101 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
     2161 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
     2221 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
     2281 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
     2341 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
     2401 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
     2461 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
     2521 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
     2581 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
     2641 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
     2701 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg
     2761 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
     2821 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     2881 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     2941 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     3001 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     3061 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     3121 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     3181 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     3241 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     3301 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     3361 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     3421 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     3481 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     3541 gttcagcttt cttgtacaaa gtggttgata tccagcacag tggcggccgc tcgagtctag
     3601 agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc
     3661 gtaccggtta gtaatgagtt tggaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     3721 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     3781 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3841 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     3901 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     3961 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     4021 cttttgcaaa aagctccccc tgttgacaat taatcatcgg catagtatat cggcatagta
     4081 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
     4141 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     4201 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     4261 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     4321 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     4381 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     4441 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagattta
     4501 aatggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     4561 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     4621 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     4681 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     4741 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     4801 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     4861 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     4921 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     4981 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     5041 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     5101 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     5161 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     5221 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     5281 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     5341 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     5401 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     5461 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     5521 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     5581 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     5641 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     5701 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     5761 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     5821 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     5881 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     5941 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     6001 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     6061 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     6121 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     6181 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     6241 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     6301 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     6361 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     6421 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     6481 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     6541 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     6601 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     6661 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     6721 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     6781 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     6841 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     6901 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     6961 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7021 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7081 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7141 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7201 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7261 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7321 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7381 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7441 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7501 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7561 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     7621 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     7681 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     7741 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     7801 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     7861 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     7921 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     7981 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     8041 ggagctgcaa gctt
//
