LOCUS       V014840                10943 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014840
VERSION     V014840
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10943)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..820
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             835..1015
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1680..1913
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2098..2142
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2291..2332
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2440..2557
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2607..2847
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        2872..2947
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     promoter        2980..3309
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3325..3711
                     /label="MS2-N55K"
                     /note="bacteriophage MS2 coat protein"
     CDS             3802..4344
                     /label="p65"
                     /note="C-terminal portion of the p65 subunit of mouse
                     NF-kappa-B"
     CDS             4369..4740
                     /label="HSF1"
                     /note="C-terminal activation domain from the human heat
                     shock transcription factor HSF1"
     CDS             4756..4809
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             4816..5607
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     misc_feature    5635..6223
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    6960..7184
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7230..7658
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7685..8001
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        8049..8096
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             8115..8486
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    8619..8752
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(8837..8867)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8882..8903)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9191..9779)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9953..10810)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10811..10915)
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagcgagg gcctatttcc catgattcct tcatatttgc
     2641 atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta aacacaaaga
     2701 tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa
     2761 aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct
     2821 tggctttata tatcttgtgg aaaggacgaa acaccggaga cggacgtctc tgttttagag
     2881 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
     2941 tcggtgcttt tttgaattcg ctagctaggt cttgaaaggg tgtgtcagtt agggtgtgga
     3001 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3061 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3121 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     3181 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     3241 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     3301 ttttgcaaac gtacggccac catggcttca aactttactc agttcgtgct cgtggacaat
     3361 ggtgggacag gggatgtgac agtggctcct tctaatttcg ctaatggggt ggcagagtgg
     3421 atcagctcca actcacggag ccaggcctac aaggtgacat gcagcgtcag gcagtctagt
     3481 gcccagaaga gaaagtatac catcaaggtg gaggtcccca aagtggctac ccagacagtg
     3541 ggcggagtcg aactgcctgt cgccgcttgg aggtcctacc tgaacatgga gctcactatc
     3601 ccaattttcg ctaccaattc tgactgtgaa ctcatcgtga aggcaatgca ggggctcctc
     3661 aaagacggta atcctatccc ttccgccatc gccgctaact caggtatcta cagcgctgga
     3721 ggaggtggaa gcggaggagg aggaagcgga ggaggaggta gcggacctaa gaaaaagagg
     3781 aaggtggcgg ccgctggatc cccttcaggg cagatcagca accaggccct ggctctggcc
     3841 cctagctccg ctccagtgct ggcccagact atggtgccct ctagtgctat ggtgcctctg
     3901 gcccagccac ctgctccagc ccctgtgctg accccaggac caccccagtc actgagcgct
     3961 ccagtgccca agtctacaca ggccggcgag gggactctga gtgaagctct gctgcacctg
     4021 cagttcgacg ctgatgagga cctgggagct ctgctgggga acagcaccga tcccggagtg
     4081 ttcacagatc tggcctccgt ggacaactct gagtttcagc agctgctgaa tcagggcgtg
     4141 tccatgtctc atagtacagc cgaaccaatg ctgatggagt accccgaagc cattacccgg
     4201 ctggtgaccg gcagccagcg gccccccgac cccgctccaa ctcccctggg aaccagcggc
     4261 ctgcctaatg ggctgtccgg agatgaagac ttctcaagca tcgctgatat ggactttagt
     4321 gccctgctgt cacagatttc ctctagtggg cagggaggag gtggaagcgg cttcagcgtg
     4381 gacaccagtg ccctgctgga cctgttcagc ccctcggtga ccgtgcccga catgagcctg
     4441 cctgaccttg acagcagcct ggccagtatc caagagctcc tgtctcccca ggagcccccc
     4501 aggcctcccg aggcagagaa cagcagcccg gattcaggga agcagctggt gcactacaca
     4561 gcgcagccgc tgttcctgct ggaccccggc tccgtggaca ccgggagcaa cgacctgccg
     4621 gtgctgtttg agctgggaga gggctcctac ttctccgaag gggacggctt cgccgaggac
     4681 cccaccatct ccctgctgac aggctcggag cctcccaaag ccaaggaccc cactgtctcc
     4741 tgtacaggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat
     4801 cccggccctg ccaccatggc tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     4861 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     4921 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     4981 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     5041 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     5101 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     5161 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     5221 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     5281 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     5341 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     5401 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     5461 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     5521 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     5581 ttctatcgcc ttcttgacga gttcttctga gaattcgata tcaagcttat cggtaatcaa
     5641 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     5701 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     5761 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     5821 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     5881 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     5941 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6001 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     6061 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6121 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     6181 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct
     6241 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     6301 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     6361 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6421 gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     6481 taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga
     6541 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa
     6601 gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat
     6661 gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg
     6721 gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg
     6781 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     6841 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     6901 ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac
     6961 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     7021 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     7081 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     7141 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     7201 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     7261 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     7321 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     7381 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     7441 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     7501 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     7561 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     7621 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     7681 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7741 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     7801 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     7861 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7921 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     7981 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     8041 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
     8101 tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg
     8161 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg
     8221 acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc
     8281 aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg
     8341 ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg
     8401 agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg
     8461 tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg
     8521 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     8581 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     8641 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     8701 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     8761 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     8821 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     8881 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8941 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     9001 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     9061 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     9121 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     9181 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     9241 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     9301 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     9361 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     9421 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     9481 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     9541 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     9601 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     9661 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     9721 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     9781 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9841 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9901 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9961 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    10021 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    10081 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    10141 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    10201 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    10261 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    10321 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    10381 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    10441 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    10501 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    10561 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    10621 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    10681 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    10741 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    10801 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    10861 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    10921 ttccccgaaa agtgccacct gac
//
