LOCUS       V010468                 7076 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V010468
VERSION     V010468
KEYWORDS    pLenti-N-tYFP.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7076)
  AUTHORS   OriGene
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7076)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
            Inserts are typically cloned between the SgfI (AsiSI) and MluI
            sites.
FEATURES             Location/Qualifiers
     source          1..7076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        237..616
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        617..819
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             834..1014
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1683..1916
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2101..2145
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2294..2335
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2443..2560
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2643..3022
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3024..3223
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        3252..3270
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3320..4048
                     /label="TurboYFP"
                     /note="enhanced derivative of PhiYFP"
     misc_feature    4049..4127
                     /label="MCS"
                     /note="MCS"
                     /note="multiple cloning site"
     protein_bind    complement(4171..4204)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4260..4848
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(4851..4867)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     LTR             5377..5557
                     /label="3' LTR (truncated)"
                     /note="3' LTR (truncated)"
                     /note="truncated 3' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(5619..6207)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6287..6943)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(6944..7048)
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat
     1561 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1621 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1681 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1741 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1801 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1861 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1921 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1981 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2041 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2101 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2161 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2221 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2281 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2341 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2401 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2461 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2521 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2581 cagagatcca gtttggttag taccgggccc gctctagaca tgtccaatat gaccgccatg
     2641 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     2701 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     2761 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     2821 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     2881 tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc
     2941 ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt
     3001 attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata
     3061 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     3121 ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca
     3181 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
     3241 tcagaatttt gtaatacgac tcactatagg gcggccggga attcgtcgac tggatccggt
     3301 accgaggaga tctgccgcca tgagcagcgg cgccctgctg ttccacggca agatccccta
     3361 cgtggtggag atggagggca atgtggatgg ccacaccttc agcatccgcg gcaagggcta
     3421 cggcgatgcc agcgtgggca aggtggatgc ccagttcatc tgcaccaccg gcgatgtgcc
     3481 cgtgccctgg agcaccctgg tgaccaccct gacctacggc gcccagtgct tcgccaagta
     3541 cggccccgag ctgaaggatt tctacaagag ctgcatgccc gatggctacg tgcaggagcg
     3601 caccatcacc ttcgagggcg atggcaattt caagacccgc gccgaggtga ccttcgagaa
     3661 tggcagcgtg tacaatcgcg tgaagctgaa tggccagggc ttcaagaagg atggccacgt
     3721 gctgggcaag aatctggagt tcaatttcac cccccactgc ctgtacatct ggggcgatca
     3781 ggccaatcac ggcctgaaga gcgccttcaa aatctgccac gagatcaccg gcagcaaggg
     3841 cgatttcatc gtggccgatc acacccagat gaataccccc atcggcggcg gccccgtgca
     3901 cgtgcccgag taccaccaca tgagctacca cgtgaagctg agcaaggatg tgaccgatca
     3961 ccgcgataat atgagcctga aggagaccgt gcgcgccgtg gattgccgca agacctacga
     4021 cttcgacgcc ggttccggag atacaagcgc gatcgccggc gcgccagatc tcaagcttaa
     4081 ctagttagcg gaccgacgcg ttaagcggcc gcactcgagg tttaaacggc cggccgcggt
     4141 ctgtacaagt aggattcgtc gagggaccta ataacttcgt atagcataca ttatacgaag
     4201 ttatacatgt ttaagggttc cggttccact aggtacaatt cgatatcaag cttatcgata
     4261 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     4321 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     4381 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     4441 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     4501 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     4561 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     4621 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     4681 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     4741 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     4801 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     4861 gacctcgatc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     4921 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     4981 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     5041 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat
     5101 ctgtggatct accacacaca aggctacttc cctgattggc agaactacac accagggcca
     5161 gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag
     5221 aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat
     5281 gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt
     5341 catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc
     5401 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     5461 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     5521 ctcagaccct tttagtcagt gtggaaaatc tctagcagca tgtgagcaaa aggccagcaa
     5581 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     5641 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     5701 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     5761 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     5821 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     5881 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     5941 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6001 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     6061 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6121 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     6181 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     6241 gctcagtgga acgaaaactc acgttaaggg attttggtca tgattacgcc ccgccctgcc
     6301 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa
     6361 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
     6421 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
     6481 tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag
     6541 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
     6601 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
     6661 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca
     6721 tacggaactc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
     6781 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
     6841 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
     6901 gggatatatc aacggtggta tatccagtga tttttttctc catactcttc ctttttcaat
     6961 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     7021 agaaaaataa acaaataggg gtcccgcgca catttccccg aaaagtgcca cctgac
//
