LOCUS       62056_13855       12226 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12226)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        17..243
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    471..596
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1089..1322
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1507..1551
                     /codon_start=1
                     /label=gp41 peptide
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et 
                     al., 2013)"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1735..1852
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        1921..2132
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2161..2226
                     /codon_start=1
                     /label=3xFLAG
                     /note="three tandem FLAG(R) epitope tags, followed by an 
                     enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2233..2253
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             3013..7113
                     /codon_start=1
                     /label=Cas9(D10A)
                     /note="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7423..7479
                     /codon_start=1
                     /label=P2A
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7480..8076
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    8095..8683
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             8755..8988
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9066..9200
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      9227..9362
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(9383..9401)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      9569..9997
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        10050..10154
                     /label=AmpR promoter
     CDS             10155..11012
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11186..11774
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    12062..12083
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        12098..12128
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12136..12152
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12160..12176
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        12197..12215
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
       61 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
      121 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga
      181 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata
      241 aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      301 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      361 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      421 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
      481 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
      541 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
      601 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
      661 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
      721 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
      781 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
      841 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
      901 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
      961 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1021 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1081 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1141 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1201 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1261 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1321 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     1381 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1441 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1501 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1561 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1621 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     1681 tcaccattat cgtttcagac ccacctccca accccgaggg gacccagacc acaattttaa
     1741 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     1801 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     1861 ttacagggac agcagagatc cactttggcg ccggctcgag tggctccggt gcccgtcagt
     1921 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     1981 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2041 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2101 tttttcgcaa cgggtttgcc gccagaacac aggtgtcgtg acgcgggatc cgccaccatg
     2161 gactataagg accacgacgg agactacaag gatcatgata ttgattacaa agacgatgac
     2221 gataagatgg ccccaaagaa gaagcggaag gtcggtatcc acggagtccc agcagccatg
     2281 agctcagaga ctggcccagt ggctgtggac cccacattga gacggcggat cgagccccat
     2341 gagtttgagg tattcttcga tccgagagag ctccgcaagg agacctgcct gctttacgaa
     2401 attaattggg ggggccggca ctccatttgg cgacatacat cacagaacac taacaagcac
     2461 gtcgaagtca acttcatcga gaagttcacg acagaaagat atttctgtcc gaacacaagg
     2521 tgcagcatta cctggtttct cagctggagc ccatgcggcg aatgtagtag ggccatcact
     2581 gaattcctgt caaggtatcc ccacgtcact ctgtttattt acatcgcaag gctgtaccac
     2641 cacgctgacc cccgcaatcg acaaggcctg cgggatttga tctcttcagg tgtgactatc
     2701 caaattatga ctgagcagga gtcaggatac tgctggagaa actttgtgaa ttatagcccg
     2761 agtaatgaag cccactggcc taggtatccc catctgtggg tacgactgta cgttcttgaa
     2821 ctgtactgca tcatactggg cctgcctcct tgtctcaaca ttctgagaag gaagcagcca
     2881 cagctgacat tctttaccat cgctcttcag tcttgtcatt accagcgact gcccccacac
     2941 attctctggg ccaccgggtt gaaaagcggc agcgagactc ccgggacctc agagtccgcc
     3001 acacccgaaa gtgacaagaa gtacagcatc ggcctggcca tcggcaccaa ctctgtgggc
     3061 tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt gctgggcaac
     3121 accgaccggc acagcatcaa gaagaacctg atcggagccc tgctgttcga cagcggcgaa
     3181 acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag acggaagaac
     3241 cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga cgacagcttc
     3301 ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga gcggcacccc
     3361 atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac catctaccac
     3421 ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat ctatctggcc
     3481 ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct gaaccccgac
     3541 aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca gctgttcgag
     3601 gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc cagactgagc
     3661 aagagcagac ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg
     3721 ttcggcaacc tgattgccct gagcctgggc ctgaccccca acttcaagag caacttcgac
     3781 ctggccgagg atgccaaact gcagctgagc aaggacacct acgacgacga cctggacaac
     3841 ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc
     3901 gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa ggcccccctg
     3961 agcgcctcta tgatcaagag atacgacgag caccaccagg acctgaccct gctgaaagct
     4021 ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca gagcaagaac
     4081 ggctacgccg gctacattga cggcggagcc agccaggaag agttctacaa gttcatcaag
     4141 cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa cagagaggac
     4201 ctgctgcgga agcagcggac cttcgacaac ggcagcatcc cccaccagat ccacctggga
     4261 gagctgcacg ccattctgcg gcggcaggaa gatttttacc cattcctgaa ggacaaccgg
     4321 gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc tctggccagg
     4381 ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac cccctggaac
     4441 ttcgaggaag tggtggacaa gggcgcttcc gcccagagct tcatcgagcg gatgaccaac
     4501 ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct gtacgagtac
     4561 ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga ccgagggaat gagaaagccc
     4621 gccttcctga gcggcgagca gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg
     4681 aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg cttcgactcc
     4741 gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata ccacgatctg
     4801 ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga cattctggaa
     4861 gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga acggctgaaa
     4921 acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg gagatacacc
     4981 ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca gtccggcaag
     5041 acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat gcagctgatc
     5101 cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc cggccagggc
     5161 gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa gaagggcatc
     5221 ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca caagcccgag
     5281 aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca gaagaacagc
     5341 cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca gatcctgaaa
     5401 gaacacccag tggaaaacac ccagctgcag aacgagaagc tgtacctgta ctacctgcag
     5461 aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc cgactacgat
     5521 gtggaccata tcgtgcctca gagctttctg aaggacgact ccatcgacaa caaggtgctg
     5581 accagaagcg acaagaaccg gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag
     5641 aagatgaaga actactggcg gcagctgctg aacgccaagc tgattaccca gagaaagttc
     5701 gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc cggcttcatc
     5761 aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat cctggactcc
     5821 cggatgaaca ctaagtacga cgagaatgac aagctgatcc gggaagtgaa agtgatcacc
     5881 ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa agtgcgcgag
     5941 atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg
     6001 atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac
     6061 gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca aggctaccgc caagtacttc
     6121 ttctacagca acatcatgaa ctttttcaag accgagatta ccctggccaa cggcgagatc
     6181 cggaagcggc ctctgatcga gacaaacggc gaaaccgggg agatcgtgtg ggataagggc
     6241 cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag
     6301 accgaggtgc agacaggcgg cttcagcaaa gagtctatcc tgcccaagag gaacagcgat
     6361 aagctgatcg ccagaaagaa ggactgggac cctaagaagt acggcggctt cgacagcccc
     6421 accgtggcct attctgtgct ggtggtggcc aaagtggaaa agggcaagtc caagaaactg
     6481 aagagtgtga aagagctgct ggggatcacc atcatggaaa gaagcagctt cgagaagaat
     6541 cccatcgact ttctggaagc caagggctac aaagaagtga aaaaggacct gatcatcaag
     6601 ctgcctaagt actccctgtt cgagctggaa aacggccgga agagaatgct ggcctctgcc
     6661 ggcgaactgc agaagggaaa cgaactggcc ctgccctcca aatatgtgaa cttcctgtac
     6721 ctggccagcc actatgagaa gctgaagggc tcccccgagg ataatgagca gaaacagctg
     6781 tttgtggaac agcacaagca ctacctggac gagatcatcg agcagatcag cgagttctcc
     6841 aagagagtga tcctggccga cgctaatctg gacaaagtgc tgtccgccta caacaagcac
     6901 cgggataagc ccatcagaga gcaggccgag aatatcatcc acctgtttac cctgaccaat
     6961 ctgggagccc ctgccgcctt caagtacttt gacaccacca tcgaccggaa gaggtacacc
     7021 agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg cctgtacgag
     7081 acacggatcg acctgtctca gctgggaggc gattcaggcg gatctactaa tctgtcagat
     7141 attattgaaa aggagaccgg taagcaactg gttatccagg aatccatcct catgctccca
     7201 gaggaggtgg aagaagtcat tgggaacaag ccggaaagcg atatactcgt gcacaccgcc
     7261 tacgacgaga gcaccgacga gaatgtcatg cttctgacta gcgacgcccc tgaatacaag
     7321 ccttgggctc tggtcataca ggatagcaac ggtgagaaca agattaagat gctctctggt
     7381 ggttctccca agaagaagag gaaagtcgct agcggcagcg gcgccaccaa cttcagcctg
     7441 ctgaagcagg ccggcgacgt ggaggagaac cccggcccca tgaccgagta caagcccacg
     7501 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     7561 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     7621 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     7681 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     7741 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     7801 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     7861 gtcggagtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     7921 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     7981 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     8041 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa cgcgttaagt cgacaatcaa
     8101 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     8161 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     8221 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     8281 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     8341 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     8401 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     8461 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     8521 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     8581 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     8641 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     8701 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     8761 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     8821 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     8881 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8941 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     9001 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     9061 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     9121 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     9181 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     9241 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     9301 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     9361 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     9421 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     9481 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     9541 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     9601 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     9661 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     9721 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     9781 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     9841 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     9901 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     9961 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
    10021 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
    10081 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
    10141 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
    10201 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
    10261 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
    10321 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
    10381 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
    10441 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
    10501 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
    10561 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    10621 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    10681 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
    10741 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    10801 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    10861 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    10921 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    10981 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    11041 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    11101 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    11161 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    11221 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    11281 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    11341 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    11401 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    11461 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    11521 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    11581 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    11641 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    11701 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    11761 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    11821 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    11881 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    11941 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    12001 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    12061 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    12121 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    12181 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagct
//
