LOCUS       V005086                 6465 bp    DNA     circular SYN 31-AUG-2023
DEFINITION  Exported.
ACCESSION   V005086
VERSION     V005086
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6465)
  AUTHORS   Swofford CA, Van Dessel N, Forbes NS.
  TITLE     Quorum-sensing Salmonella selectively trigger protein expression
            within tumors
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 112 (11), 3457-3462 (2015)
   PUBMED   25737556
REFERENCE   2  (bases 1 to 6465)
  AUTHORS   Swofford CA, Van Dessel N, Forbes NS.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2014) Chemical Engineering, University of
            Massachusetts Amherst, 686 North Pleasant Street, Amherst, MA
            01003-9303, USA
REFERENCE   3  (bases 1 to 6465)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6465)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2015"; volume: "112"; issue: "11"; pages:
            "3457-3462"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-DEC-2014) Chemical Engineering, University of Massachusetts
            Amherst, 686 North Pleasant Street, Amherst, MA 01003-9303, USA"
            SGRef: number: 3; type: "Journal Article"
            SGRef: number: 1; type: ""Journal Article""; journalName: ""Proc.
            Natl. Acad. Sci. U.S.A.""; date: ""2015""; volume: ""112""; issue:
            ""11""; pages: ""3457-3462""
            SGRef: number: 2; type: ""Journal Article""; journalName:
            ""Submitted (18-DEC-2014) Chemical Engineering, University of
            Massachusetts Amherst, 686 North Pleasant Street, Amherst, MA
            01003-9303, USA"" SGRef: number: 3; type: ""Journal Article""
FEATURES             Location/Qualifiers
     source          1..6465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    133..154
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        169..199
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    207..223
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     231..247
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             275..988
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     oriT            1615..1702
                     /label="RSF1010 oriT"
                     /note="origin of transfer of the broad-host-range plasmid
                     RSF1010 (Scholz et al., 1989)"
     CDS             1783..3132
                     /codon_start=1
                     /label="DNA primase"
                     /translation="MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESG
                     HMPEFVERPADYWDAADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGAER
                     LPYTLAIHAGGGENPHCHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEALKPK
                     AWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGRGIRT
                     DRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTGR
                     RSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGV
                     ALEAMANRDAGFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQ
                     LKAMGCERSSASSLTDSLRSVVRLRRAVSAHSKAVIRLSTESGDNAGKNM"
     rep_origin      complement(3186..3774)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3948..4805)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4806..4910)
                     /label="AmpR promoter"
     CDS             complement(5090..6193)
                     /gene="asd"
                     /label="Aspartate-semialdehyde dehydrogenase"
                     /note="Aspartate-semialdehyde dehydrogenase from Salmonella
                     typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
                     Accession#: P0A1F8"
ORIGIN
        1 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
       61 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
      121 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
      181 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
      241 ctatgacctc tagatttaag aaggagatat acatatgagt aaaggagaag aacttttcac
      301 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
      361 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac
      421 tactggaaaa ctacctgttc catggccaac acttgtcact actttcgggt atggtgttca
      481 atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc
      541 cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg
      601 tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga
      661 ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa
      721 tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca
      781 caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg
      841 cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa
      901 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat
      961 tacacatggc atggatgaac tatacaaata aatctcctgc aggcatgcaa gcttaatgcg
     1021 gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg
     1081 cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc
     1141 cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg
     1201 gcgtgctgct agcgctttta gccgctttag cggcctttcc ccctacccga agggtggggg
     1261 cgcgtgtgca gccccgcagg gcctgtctcg gtcgatcatt cagcccggct catccttctg
     1321 gcgtggcggc agaccgaaca aggcgcggtc gtggtcgcgt tcaaggtacg catccattgc
     1381 cgccatgagc cgatcctccg gccactcgct gctgttcacc ttggccaaaa tcatggcccc
     1441 caccagcacc ttgcgccttg tttcgttctt gcgctcttgc tgctgttccc ttgcccgcac
     1501 ccgctgaatt tcggcattga ttcgcgctcg ttgttcttcg agcttggcca gccgatccgc
     1561 cgccttgttg ctccccttaa ccatcttgac accccattgt taatgtgctg tctcgtaggc
     1621 tatcatggag gcacagcggc ggcaatcccg accctacttt gtaggggagg gcgcacttac
     1681 cggtttctct tcgagaaact ggcctaacgg ccacccttcg ggcggtgcgc tctccgaggg
     1741 ccattgcatg gagccgaaaa gcaaaagcaa cagcgaggca gcatggcgat ttatcacctt
     1801 acggcgaaaa ccggcagcag gtcgggcggc caatcggcca gggccaaggc cgactacatc
     1861 cagcgcgaag gcaagtatgc ccgcgacatg gatgaagtct tgcacgccga atccgggcac
     1921 atgccggagt tcgtcgagcg gcccgccgac tactgggatg ctgccgacct gtatgaacgc
     1981 gccaatgggc ggctgttcaa ggaggtcgaa tttgccctgc cggtcgagct gaccctcgac
     2041 cagcagaagg cgctggcgtc cgagttcgcc cagcacctga ccggtgccga gcgcctgccg
     2101 tatacgctgg ccatccatgc cggtggcggc gagaacccgc actgccacct gatgatctcc
     2161 gagcggatca atgacggcat cgagcggccc gccgctcagt ggttcaagcg gtacaacggc
     2221 aagaccccgg agaagggcgg ggcacagaag accgaagcgc tcaagcccaa ggcatggctt
     2281 gagcagaccc gcgaggcatg ggccgaccat gccaaccggg cattagagcg ggctggccac
     2341 gacgcccgca ttgaccacag aacacttgag gcgcagggca tcgagcgcct gcccggtgtt
     2401 cacctggggc cgaacgtggt ggagatggaa ggccggggca tccgcaccga ccgggcagac
     2461 gtggccctga acatcgacac cgccaacgcc cagatcatcg acttacagga ataccgggag
     2521 gcaatagacc atgaacgcaa tcgacagagt gaagaaatcc agaggcatca acgagttagc
     2581 ggagcagatc gaaccgctgg cccagagcat ggcgacactg gccgacgaag cccggcaggt
     2641 catgagccag acccagcagg ccagcgaggc gcaggcggcg gagtggctga aagcccagcg
     2701 ccagacaggg gcggcatggg tggagctggc caaagagttg cgggaggtag ccgccgaggt
     2761 gagcagcgcc gcgcagagcg cccggagcgc gtcgcggggg tggcactgga agctatggct
     2821 aaccgtgatg ctggcttcca tgatgcctac ggtggtgctg ctgatcgcat cgttgctctt
     2881 gctcgacctg acgccactga caaccgagga cggctcgatc tggctgcgct tggtggcccg
     2941 atgaagaacg acaggacttt gcaggccata ggccgacagc tcaaggccat gggctgtgag
     3001 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     3061 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     3121 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3181 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3241 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3301 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3361 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3421 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3481 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3541 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3601 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     3661 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3721 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3781 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3841 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3901 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     3961 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     4021 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     4081 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     4141 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     4201 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     4261 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     4321 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     4381 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     4441 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     4501 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4561 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4621 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4681 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4741 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4801 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     4861 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     4921 cgaaaagtgc cacctgacgt catctgcgtt tactcctgta ttacgcaaaa aaagggtgac
     4981 gatgcacccc tggtttaaca caaatttccc cgccggatgg cggcgctgac gcgccttatc
     5041 cggcctacag aaccacacgc aggcccgata agcgctgcaa tagccactac gccaactggc
     5101 gcagcattcg acgcagcggc tcggcggcgc cccataacaa ctggtcgcct acggtaaacg
     5161 ccgacaagaa ctctggcccc atgttcagct tacgcagacg accaaccggc gtagtcaacg
     5221 tgccggtcac cgccgccggg gttaattcgc gcatagtgat atcacgatcg ttcggcacca
     5281 ctttcgccca cggattatgt gccgccagca gttcttccac cgtcggaatg gatacctctt
     5341 ttttcagctt gatggtgaac gcctggctgt gacagcgcag cgcgccgacg cgcacacaca
     5401 aaccatcaac cggaatcaca gaggcagtat tgagaatctt gttggtttcc gcctggcctt
     5461 tccactcttc gcggctctgg ccgttatcga gctgtttgtc gatccagggg atcaggcttc
     5521 ccgccagcgg tacgccaaag ttatcaaccg gcagctcgcc gctgcgggtc aatgccgtaa
     5581 ctttgcgttc aatatcaaga attgcggaag acggcgtcgc cagttcatcg gcgacatggc
     5641 catacaactg acccatctgg gttaacagct cgcgcatatg gcgcgcgccg ccgccggagg
     5701 cggcctgata ggtcgcgacg gatacccagt caacgagatt atgggcaaag agaccgccca
     5761 gcgacatcaa catcaggcta acggtacagt taccgcccac aaaggtcttc acgccattgt
     5821 tcaggccgtc ggtaatcacg tcctggttga ccgggtcgag aataataatg gcatcatctt
     5881 tcatgcgcag cgtagaagcc gcatcaatcc agtaaccctg ccatccgctt tcgcgcagct
     5941 ttggataaat ttcgttggta taatcgccgc cctggcaggt cacgatgata tcgagcgctt
     6001 ttagcgcatc cagatcaaaa gcgtcctgta gcgtgccggt ggaggtgtcg ccgaaggtgg
     6061 gcgccgcctg tccaaactgg gaggtagaaa agaaaacagg gcgaatagcg tcgaaatcgc
     6121 gctcctctac catgcgttgc atgagaacag agccgaccat tccgcgccag ccgataaaac
     6181 caacattttt catagcgttt ttttcctgca aagagatgtg ctgtgtatgc gcgccagtat
     6241 cctgtggcgc atccttcacc atacaaaaag cagccaaagt cgcaagtgaa attaatcaat
     6301 gatagcgaag ccatcagtaa tgcgacttat cctgccttac cgcgcaagca gaaagtccag
     6361 tggcgattat cagggaattt gcgttaggaa gggaatcatt tccgtcgctg ttttattgat
     6421 cctaattagg ggtttgaggg ccaatggaac gaaaacgtac gaatt
//
