LOCUS       V014808                 5413 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014808
VERSION     V014808
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    288..309
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        324..354
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    362..378
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     386..402
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     RBS             451..473
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             492..509
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             519..533
                     /label="enterokinase site"
                     /note="enterokinase recognition and cleavage site"
     CDS             534..3182
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
     promoter        complement(3548..3576)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     promoter        3689..3793
                     /label="AmpR promoter"
     CDS             3794..4651
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4825..5413
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      241 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      301 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      361 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt
      421 gcatgcctgc aggtcgactc tagaaataat tttgtttaac tttaagaagg agatatacca
      481 tgggcagcag ccatcatcat catcatcaca gcagcggcga tgacgacgac aagatgaaca
      541 cgattaacat cgctaagaac gacttctctg acatcgaact ggctgctatc ccgttcaaca
      601 ctctggctga ccattacggt gagcgtttag ctcgcgaaca gttggccctt gagcatgagt
      661 cttacgagat gggtgaagca cgcttccgca agatgtttga gcgtcaactt aaagctggtg
      721 aggttgcgga taacgctgcc gccaagcctc tcatcactac cctactccct aagatgattg
      781 cacgcatcaa cgactggttt gaggaagtga aagctaagcg cggcaagcgc ccgacagcct
      841 tccagttcct gcaagaaatc aagccggaag ccgtagcgta catcaccatt aagaccactc
      901 tggcttgcct aaccagtgct gacaatacaa ccgttcaggc tgtagcaagc gcaatcggtc
      961 gggccattga ggacgaggct cgcttcggtc gtatccgtga ccttgaagct aagcacttca
     1021 agaaaaacgt tgaggaacaa ctcaacaagc gcgtagggca cgtctacaag aaagcattta
     1081 tgcaagttgt cgaggctgac atgctctcta agggtctact cggtggcgag gcgtggtctt
     1141 cgtggcataa ggaagactct attcatgtag gagtacgctg catcgagatg ctcattgagt
     1201 caaccggaat ggttagctta caccgccaaa atgctggcgt agtaggtcaa gactctgaga
     1261 ctatcgaact cgcacctgaa tacgctgagg ctatcgcaac ccgtgcaggt gcgctggctg
     1321 gcatctctcc gatgttccaa ccttgcgtag ttcctcctaa gccgtggact ggcattactg
     1381 gtggtggcta ttgggctaac ggtcgtcgtc ctctggcgct ggtgcgtact cacagtaaga
     1441 aagcactgat gcgctacgaa gacgtttaca tgcctgaggt gtacaaagcg attaacattg
     1501 cgcaaaacac cgcatggaaa atcaacaaga aagtcctagc ggtcgccaac gtaatcacca
     1561 agtggaagca ttgtccggtc gaggacatcc ctgcgattga acgtgaagaa ctcccgatga
     1621 aaccggaaga catcgacatg aatcctgagg ctctcaccgc gtggaaacgt gctgccgctg
     1681 ctgtgtaccg caaggacaag gctcgcaagt ctcgccgtat cagccttgag ttcatgcttg
     1741 agcaagccaa taagtttgct aaccataagg ccatctggtt cccttacaac atggactggc
     1801 gcggtcgtgt ttacgctgtg tcaatgttca acccgcaagg taacgatatg accaaaggac
     1861 tgcttacgct ggcgaaaggt aaaccaatcg gtaaggaagg ttactactgg ctgaaaatcc
     1921 acggtgcaaa ctgtgcgggt gtcgataagg ttccgttccc tgagcgcatc aagttcattg
     1981 aggaaaacca cgagaacatc atggcttgcg ctaagtctcc actggagaac acttggtggg
     2041 ctgagcaaga ttctccgttc tgcttccttg cgttctgctt tgagtacgct ggggtacagc
     2101 accacggcct gagctataac tgctcccttc cgctggcgtt tgacgggtct tgctctggca
     2161 tccagcactt ctccgcgatg ctccgagatg aggtaggtgg tcgcgcggtt aacttgcttc
     2221 ctagtgaaac cgttcaggac atctacggga ttgttgctaa gaaagtcaac gagattctac
     2281 aagcagacgc aatcaatggg accgataacg aagtagttac cgtgaccgat gagaacactg
     2341 gtgaaatctc tgagaaagtc aagctgggca ctaaggcact ggctggtcaa tggctggctt
     2401 acggtgttac tcgcagtgtg actaagcgtt cagtcatgac gctggcttac gggtccaaag
     2461 agttcggctt ccgtcaacaa gtgctggaag ataccattca gccagctatt gattccggca
     2521 agggtctgat gttcactcag ccgaatcagg ctgctggata catggctaag ctgatttggg
     2581 aatctgtgag cgtgacggtg gtagctgcgg ttgaagcaat gaactggctt aagtctgctg
     2641 ctaagctgct ggctgctgag gtcaaagata agaagactgg agagattctt cgcaagcgtt
     2701 gcgctgtgca ttgggtaact cctgatggtt tccctgtgtg gcaggaatac aagaagccta
     2761 ttcagacgcg cttgaacctg atgttcctcg gtcagttccg cttacagcct accattaaca
     2821 ccaacaaaga tagcgagatt gatgcacaca aacaggagtc tggtatcgct cctaactttg
     2881 tacacagcca agacggtagc caccttcgta agactgtagt gtgggcacac gagaagtacg
     2941 gaatcgaatc ttttgcactg attcacgact ccttcggtac cattccggct gacgctgcga
     3001 acctgttcaa agcagtgcgc gaaactatgg ttgacacata tgagtcttgt gatgtactgg
     3061 ctgatttcta cgaccagttc gctgaccagt tgcacgagtc tcaattggac aaaatgccag
     3121 cacttccggc taaaggtaac ttgaacctcc gtgacatctt agagtcggac ttcgcgttcg
     3181 cgtaacgcca aatcaatacg actccggatc cacaggacgg gtgtggtcgc catgatcgcg
     3241 tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg
     3301 gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc
     3361 acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt
     3421 accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg
     3481 agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa
     3541 ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa
     3601 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     3661 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3721 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3781 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     3841 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     3901 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     3961 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     4021 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     4081 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     4141 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     4201 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     4261 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     4321 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     4381 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     4441 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     4501 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     4561 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     4621 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     4681 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     4741 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4801 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgtgcgtaa tctgctgctt
     4861 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4921 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     4981 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5041 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5101 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5161 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5221 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5281 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5341 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5401 gagcctatgg aaa
//
