LOCUS       62056_16355        5671 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        108..139
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             172..1821
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(1868..1989)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2183..2540
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2571..3362
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3390..3438
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(3765..4353)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4556..5413)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    5580..5671
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtaccg ctagcctcga tccagggttt aaataagttc atcagccagg
       61 gtttaaataa gttcatcagg cgcgtagatc tgcagaagct tagacactag agggtatata
      121 atggaagctc gacttccagc ttggcaatcc ggtactgttg gtaaagccac catggaagat
      181 gccaaaaaca ttaagaaggg cccagcgcca ttctacccac tcgaagacgg gaccgccggc
      241 gagcagctgc acaaagccat gaagcgctac gccctggtgc ccggcaccat cgcctttacc
      301 gacgcacata tcgaggtgga cattacctac gccgagtact tcgagatgag cgttcggctg
      361 gcagaagcta tgaagcgcta tgggctgaat acaaaccatc ggatcgtggt gtgcagcgag
      421 aatagcttgc agttcttcat gcccgtgttg ggtgccctgt tcatcggtgt ggctgtggcc
      481 ccagctaacg acatctacaa cgagcgcgag ctgctgaaca gcatgggcat cagccagccc
      541 accgtcgtat tcgtgagcaa gaaagggctg caaaagatcc tcaacgtgca aaagaagcta
      601 ccgatcatac aaaagatcat catcatggat agcaagaccg actaccaggg cttccaaagc
      661 atgtacacct tcgtgacttc ccatttgcca cccggcttca acgagtacga cttcgtgccc
      721 gagagcttcg accgggacaa aaccatcgcc ctgatcatga acagtagtgg cagtaccgga
      781 ttgcccaagg gcgtagccct accgcaccgc accgcttgtg tccgattcag tcatgcccgc
      841 gaccccatct tcggcaacca gatcatcccc gacaccgcta tcctcagcgt ggtgccattt
      901 caccacggct tcggcatgtt caccacgctg ggctacttga tctgcggctt tcgggtcgtg
      961 ctcatgtacc gcttcgagga ggagctattc ttgcgcagct tgcaagacta taagattcaa
     1021 tctgccctgc tggtgcccac actatttagc ttcttcgcta agagcactct catcgacaag
     1081 tacgacctaa gcaacttgca cgagatcgcc agcggcgggg cgccgctcag caaggaggta
     1141 ggtgaggccg tggccaaacg cttccaccta ccaggcatcc gccagggcta cggcctgaca
     1201 gaaacaacca gcgccattct gatcaccccc gaaggggacg acaagcctgg cgcagtaggc
     1261 aaggtggtgc ccttcttcga ggctaaggtg gtggacttgg acaccggtaa gacactgggt
     1321 gtgaaccagc gcggcgagct gtgcgtccgt ggccccatga tcatgagcgg ctacgttaac
     1381 aaccccgagg ctacaaacgc tctcatcgac aaggacggct ggctgcacag cggcgacatc
     1441 gcctactggg acgaggacga gcacttcttc atcgtggacc ggctgaagag cctgatcaaa
     1501 tacaagggct accaggtagc cccagccgaa ctggagagca tcctgctgca acaccccaac
     1561 atcttcgacg ccggggtcgc cggcctgccc gacgacgatg ccggcgagct gcccgccgca
     1621 gtcgtcgtgc tggaacacgg taaaaccatg accgagaagg agatcgtgga ctatgtggcc
     1681 agccaggtta caaccgccaa gaagctgcgc ggtggtgttg tgttcgtgga cgaggtgcct
     1741 aaaggactga ccggcaagtt ggacgcccgc aagatccgcg agattctcat taaggccaag
     1801 aagggcggca agatcgccgt gtaataattc tagagtcggg gcggccggcc gcttcgagca
     1861 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     1921 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     1981 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     2041 gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccgttt
     2101 gcgtattggg cgctcttccg ctgatctgcg cagcaccatg gcctgaaata acctctgaaa
     2161 gaggaacttg gttagctacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
     2221 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     2281 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     2341 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     2401 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     2461 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     2521 gaggcctagg cttttgcaaa aagctcgatt cttctgacac tagcgccacc atgatcgaac
     2581 aagacggcct ccatgctggc agtcccgcag cttgggtcga acgcttgttc gggtacgact
     2641 gggcccagca gaccatcgga tgtagcgatg cggccgtgtt ccgtctaagc gctcaaggcc
     2701 ggcccgtgct gttcgtgaag accgacctga gcggcgccct gaacgagctt caagacgagg
     2761 ctgcccgcct gagctggctg gccaccaccg gcgtaccctg cgccgctgtg ttggatgttg
     2821 tgaccgaagc cggccgggac tggctgctgc tgggcgaggt ccctggccag gatctgctga
     2881 gcagccacct tgcccccgct gagaaggttt ctatcatggc cgatgcaatg cggcgcctgc
     2941 acaccctgga ccccgctacc tgccccttcg accaccaggc taagcatcgg atcgagcgtg
     3001 ctcggacccg catggaggcc ggcctggtgg accaggacga cctggacgag gagcatcagg
     3061 gcctggcccc cgctgaactg ttcgcccgac tgaaagcccg catgccggac ggtgaggacc
     3121 tggttgtcac acacggagat gcctgcctcc ctaacatcat ggtcgagaat ggccgcttct
     3181 ccggcttcat cgactgcggt cgcctaggag ttgccgaccg ctaccaggac atcgccctgg
     3241 ccacccgcga catcgctgag gagcttggcg gcgagtgggc cgaccgcttc ttagtcttgt
     3301 acggcatcgc agctcccgac agccagcgca tcgccttcta ccgcttgctc gacgagttct
     3361 tttaatgatc tagaaccggt catggccgca ataaaatatc tttattttca ttacatctgt
     3421 gtgttggttt tttgtgtgtt cgaactagat gctgtcgacc gatgcccttg agagccttca
     3481 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
     3541 tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc
     3601 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     3661 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     3721 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3781 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3841 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3901 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     3961 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     4021 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4081 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     4141 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     4201 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     4261 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     4321 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     4381 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     4441 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     4501 tatgagtaaa cttggtctga cagcggccgc aaatgctaaa ccactgcagt ggttaccagt
     4561 gcttgatcag tgaggcaccg atctcagcga tctgcctatt tcgttcgtcc atagtggcct
     4621 gactccccgt cgtgtagatc actacgattc gtgagggctt accatcaggc cccagcgcag
     4681 caatgatgcc gcgagagccg cgttcaccgg cccccgattt gtcagcaatg aaccagccag
     4741 cagggagggc cgagcgaaga agtggtcctg ctactttgtc cgcctccatc cagtctatga
     4801 gctgctgtcg tgatgctaga gtaagaagtt cgccagtgag tagtttccga agagttgtgg
     4861 ccattgctac tggcatcgtg gtatcacgct cgtcgttcgg tatggcttcg ttcaactctg
     4921 gttcccagcg gtcaagccgg gtcacatgat cacccatatt atgaagaaat gcagtcagct
     4981 ccttagggcc tccgatcgtt gtcagaagta agttggccgc ggtgttgtcg ctcatggtaa
     5041 tggcagcact acacaattct cttaccgtca tgccatccgt aagatgcttt tccgtgaccg
     5101 gcgagtactc aaccaagtcg ttttgtgagt agtgtatacg gcgaccaagc tgctcttgcc
     5161 cggcgtctat acgggacaac accgcgccac atagcagtac tttgaaagtg ctcatcatcg
     5221 ggaatcgttc ttcggggcgg aaagactcaa ggatcttgcc gctattgaga tccagttcga
     5281 tatagcccac tcttgcaccc agttgatctt cagcatcttt tactttcacc agcgtttcgg
     5341 ggtgtgcaaa aacaggcaag caaaatgccg caaagaaggg aatgagtgcg acacgaaaat
     5401 gttggatgct catactcgtc ctttttcaat attattgaag catttatcag ggttactagt
     5461 acgtctctca aggataagta agtaatatta aggtacggga ggtattggac aggccgcaat
     5521 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta
     5581 acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc
     5641 agtgcaagtg caggtgccag aacatttctc t
//
