LOCUS       V029093                 9984 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029093
VERSION     V029093
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        65..368
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        369..572
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     protein_bind    595..619
                     /bound_moiety="BP Clonase™"
                     /gene="mutant version of&nbsp;attB"
                     /note="recombination site for the Gateway® BP reaction"
                     /label="attB1"
     CDS             619..2883
                     /label="SENP5(NM_152699)"
                     /note="SENP5(NM_152699)"
                     /gene="SENP5"
     protein_bind    complement(2882..2906)
                     /bound_moiety="BP Clonase™"
                     /gene="mutant version of&nbsp;attB"
                     /note="recombination site for the Gateway® BP reaction"
                     /label="attB2"
     CDS             2908..2949
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /transl_table=1
                     /translation="GKPIPNPLLGLDST"
                     /label="V5 tag"
     misc_feature    2991..3579
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             3651..3884
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    3962..4083
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      4123..4258
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     promoter        complement(4279..4297)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(4307..4323)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     rep_origin      4465..4920
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4946..5050
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             5051..5911
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      6082..6670
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     protein_bind    6958..6979
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        6994..7024
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7032..7048
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     7056..7072
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     promoter        7093..7111
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
     promoter        7139..7365
                     /note="Rous sarcoma virus enhancer/promoter"
                     /label="RSV promoter"
     LTR             7366..7546
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    7593..7718
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    8211..8444
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             8629..8673
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     promoter        8848..9348
                     /note="human phosphoglycerate kinase 1 promoter"
                     /label="hPGK promoter"
     CDS             9360..9755
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /note="confers resistance to blasticidin"
                     /product="blasticidin S deaminase"
                     /transl_table=1
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
                     /label="BSD"
     misc_feature    9815..9932
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtacaaaa aagttggcat gaaaaaacag aggaaaattc tatggaggaa aggaatccac
      661 ttagcctttt ctgagaaatg gaatactggg tttggaggct ttaagaagtt ttattttcac
      721 caacacttgt gcattctgaa agctaagctg ggaaggccag ttacttggaa tagacagttg
      781 agacatttcc agggtagaaa gaaagctctt caaatccaga aaacgtggat caaggatgaa
      841 cccctttgtg ctaagaccaa gttcaatgtg gctactcaaa atgttagtac tttgtcctct
      901 aaagtgaaaa gaaaggacgc taaacacttc atttcctcct caaagactct cctgagactc
      961 caagcagaga agctgttgtc atcagcaaag aattctgacc atgaatactg cagagagaaa
     1021 aatctcttga aggcagttac tgactttcca tcaaatagtg ctttaggtca ggccaatggt
     1081 cacagaccta ggacagaccc acaaccttct gactttccca tgaagttcaa tggggagagc
     1141 caaagtccag gtgagagtgg cacgattgtg gtcaccttga acaaccataa gagaaagggc
     1201 ttttgttacg gctgctgcca agggccggag caccacagga atgggggacc cttgattcca
     1261 aaaaagttcc aacttaacca acatagaagg ataaaattat ctcctcttat gatgtatgag
     1321 aaattatcca tgattagatt tcggtacagg attctcagat cccagcactt cagaaccaaa
     1381 agcaaggttt gcaagctaag aaaagcccag cgaagctggg tacagaaagt cactggggac
     1441 catcaagaga cccgtaggga gaacggtgag ggtggcagtt gcagcccatt tccttcccca
     1501 gaacctaaag acccttcttg tcggcatcag ccgtactttc cagatatgga cagcagtgct
     1561 gtggtgaagg ggacgaactc tcatgtgcct gattgccaca ctaaaggaag ctctttcttg
     1621 ggcaaggagc ttagtttaga cgaagcattc cctgaccaac agaatggcag tgccacaaac
     1681 gcctgggacc agtcatcctg ttcttctcct aagtgggagt gtacagagct gattcatgac
     1741 atccccttac cagaacatcg ttctaatacc atgttcattt cagaaactga aagagaaatt
     1801 atgactctgg gtcaggaaaa tcagacaagt tctgtcagtg atgacagagt aaaactgtca
     1861 gtgtctggag cagatacatc tgtgagtagc gtagatgggc ctgtgtccca aaaggctgtt
     1921 caaaatgaga actcatacca gatggaggag gatggatctc tcaagcagag cattcttagt
     1981 tctgagttgc tggaccaccc ttactgtaaa agtccactgg aggctccctt ggtgtgcagt
     2041 ggactcaaac tagaaaatca agtaggaggt ggaaagaaca gtcagaaagc ctctccagtg
     2101 gatgatgaac agctgtcagt ctgtctttct ggattcctag atgaggttat gaagaagtat
     2161 ggcagtttgg ttccactcag tgaaaaagaa gtccttggaa gattaaaaga tgtctttaat
     2221 gaagactttt ctaatagaaa accatttatc aatagggaaa taacaaacta tcgggccaga
     2281 catcaaaaat gtaacttccg tatcttctat aataaacaca tgctggatat ggacgacctg
     2341 gcgactctgg atggtcagaa ctggctgaat gaccaggtca ttaatatgta tggtgagctg
     2401 ataatggatg cagtcccaga caaagttcac ttcttcaaca gcttttttca tagacagctg
     2461 gtaaccaaag gatataatgg agtaaaaaga tggactaaaa aggtggattt gtttaaaaag
     2521 agtcttctgt tgattcctat tcacctggaa gtccactggt ctctcattac tgtgacactc
     2581 tctaatcgaa ttatttcatt ttatgattcc caaggcattc attttaagtt ttgtgtagag
     2641 aatataagaa agtatttgct gactgaagcc agagaaaaaa atagacctga atttcttcag
     2701 ggttggcaga ctgctgttac gaagtgtatt ccacaacaga aaaacgacag tgactgtgga
     2761 gtctttgtgc tccagtactg caagtgcctc gccttagagc agcctttcca gttttcacaa
     2821 gaagacatgc cccgagtgcg gaagaggatt tacaaggagc tatgtgagtg ccggctcatg
     2881 gacccaactt tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc
     2941 gattctacgt agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc
     3001 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     3061 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     3121 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     3181 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     3241 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     3301 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     3361 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     3421 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     3481 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     3541 ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac
     3601 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     3661 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     3721 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     3781 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     3841 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc
     3901 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     3961 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4021 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4081 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     4141 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     4201 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     4261 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     4321 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     4381 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     4441 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4501 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     4561 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4621 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4681 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     4741 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     4801 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     4861 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     4921 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4981 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5041 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5101 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     5161 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     5221 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     5281 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     5341 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     5401 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     5461 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     5521 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     5581 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     5641 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     5701 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     5761 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     5821 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     5881 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     5941 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6001 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6061 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6121 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6181 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     6241 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6301 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6361 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6421 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6481 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6541 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6601 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6661 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6721 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     6781 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     6841 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     6901 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     6961 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     7021 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     7081 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     7141 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     7201 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     7261 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     7321 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     7381 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     7441 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     7501 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     7561 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     7621 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     7681 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     7741 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     7801 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     7861 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     7921 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     7981 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     8041 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     8101 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     8161 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     8221 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     8281 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     8341 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     8401 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     8461 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     8521 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     8581 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     8641 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     8701 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     8761 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     8821 acccacctcc caaccccgag gggacccttg cgccttttcc aaggcagccc tgggtttgcg
     8881 cagggacgcg gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct
     8941 cgcacattct tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc
     9001 cccccggcga cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg
     9061 tgccggacgt gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc
     9121 cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca
     9181 gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta
     9241 gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg
     9301 tcggcagtcg gctccctcgt tgaccgaatc accgacctct ctccccaggg ggtaccacca
     9361 tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca
     9421 acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc
     9481 gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg
     9541 tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa
     9601 atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc
     9661 atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc
     9721 gtgaattgct gccctctggt tatgtgtggg agggcctgca gctgcagtag taagaattct
     9781 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     9841 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     9901 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     9961 cactttggcg ccggctcgag gggg
//
