LOCUS       V041805                10857 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041805
VERSION     V041805
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    135..156
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        171..201
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    209..225
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     233..249
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     promoter        270..288
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
     promoter        316..542
                     /note="Rous sarcoma virus enhancer/promoter"
                     /label="RSV promoter"
     LTR             543..723
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    770..895
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1388..1621
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1806..1850
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     promoter        2025..2525
                     /note="human phosphoglycerate kinase 1 promoter"
                     /label="hPGK promoter"
     CDS             2537..2932
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /note="confers resistance to blasticidin"
                     /product="blasticidin S deaminase"
                     /transl_table=1
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
                     /label="BSD"
     misc_feature    2992..3109
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     enhancer        3226..3529
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        3530..3733
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     protein_bind    3756..3780
                     /bound_moiety="BP Clonase™"
                     /gene="mutant version of&nbsp;attB"
                     /note="recombination site for the Gateway® BP reaction"
                     /label="attB1"
     CDS             3801..3824
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     CDS             3831..6869
                     /label="ELAPOR1(NM_020775)"
                     /note="ELAPOR1(NM_020775)"
                     /gene="ELAPOR1"
     CDS             6882..6908
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein
                     (Fischer et al., 1995)"
                     /transl_table=1
                     /translation="LPPLERLTL"
                     /label="NES"
     protein_bind    complement(6916..6940)
                     /bound_moiety="BP Clonase™"
                     /gene="mutant version of&nbsp;attB"
                     /note="recombination site for the Gateway® BP reaction"
                     /label="attB2"
     CDS             6942..6983
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /transl_table=1
                     /translation="GKPIPNPLLGLDST"
                     /label="V5 tag"
     misc_feature    7025..7613
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7685..7918
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7996..8117
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8157..8292
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     promoter        complement(8313..8331)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(8341..8357)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     rep_origin      8499..8954
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        8980..9084
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             9085..9945
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      10116..10704
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
       61 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
      121 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
      181 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
      241 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
      301 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
      361 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
      421 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
      481 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
      541 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
      601 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
      661 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
      721 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
      781 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
      841 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
      901 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
      961 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     1021 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     1081 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     1141 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     1201 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     1261 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     1321 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     1381 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     1441 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     1501 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     1561 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     1621 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     1681 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     1741 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     1801 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     1861 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     1921 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     1981 caccattatc gtttcagacc cacctcccaa ccccgagggg acccttgcgc cttttccaag
     2041 gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg
     2101 gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg
     2161 ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt
     2221 ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc
     2281 tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca
     2341 atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg
     2401 cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc gcattctgca
     2461 agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc gacctctctc
     2521 cccagggggt accaccatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag
     2581 agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc
     2641 tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc
     2701 ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg
     2761 tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca
     2821 ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc
     2881 gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg gcctgcagct
     2941 gcagtagtaa gaattctaga tcttgagaca aatggcagta ttcatccaca attttaaaag
     3001 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     3061 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     3121 cagggacagc agagatccac tttggcgccg gctcgagggg gcccggggtt attaatagta
     3181 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     3241 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     3301 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     3361 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     3421 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     3481 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     3541 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     3601 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     3661 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     3721 tataagcaga gctctctggc taactgtcgg gatcaacaag tttgtacaaa aaagcaggct
     3781 tcgaaggaga tagaaccatg gactacaagg atgacgacga taagggatcc atggctgagc
     3841 ctgggcacag ccaccatctc tccgccagag tcaggggaag aactgagagg cgcatacccc
     3901 ggctgtggcg gctgctgctc tgggctggga ccgccttcca ggtgacccag ggaacgggac
     3961 cggagcttca tgcctgcaaa gagtctgagt accactatga gtacacggcg tgtgacagca
     4021 cgggttccag gtggagggtc gccgtgccgc ataccccggg cctgtgcacc agcctgcctg
     4081 accccatcaa gggcaccgag tgctccttct cctgcaacgc cggggagttt ctggatatga
     4141 aggaccagtc atgtaagcca tgcgctgagg gccgctactc cctcggcaca ggcattcggt
     4201 ttgatgagtg ggatgagctg ccccatggct ttgccagcct ctcagccaac atggagctgg
     4261 atgacagtgc tgctgagtcc accgggaact gtacttcgtc caagtgggtt ccccggggcg
     4321 actacatcgc ctccaacacg gacgaatgca cagccacact gatgtacgcc gtcaacctga
     4381 agcaatctgg caccgttaac ttcgaatact actatccaga ctccagcatc atctttgagt
     4441 ttttcgttca gaatgaccag tgccagccca atgcagatga ctccaggtgg atgaagacca
     4501 cagagaaagg atgggaattc cacagtgtgg agctaaatcg aggcaataat gtcctctatt
     4561 ggagaaccac agccttctca gtatggacca aagtacccaa gcctgtgctg gtgagaaaca
     4621 ttgccataac aggggtggcc tacacttcag aatgcttccc ctgcaaacct ggcacgtatg
     4681 cagacaagca gggctcctct ttctgcaaac tttgcccagc caactcttat tcaaataaag
     4741 gagaaacttc ttgccaccag tgtgaccctg acaaatactc agagaaagga tcttcttcct
     4801 gtaacgtgcg cccagcttgc acagacaaag attatttcta cacacacacg gcctgcgatg
     4861 ccaacggaga gacacaactc atgtacaaat gggccaagcc gaaaatctgt agcgaggacc
     4921 ttgagggggc agtgaagctg cctgcctctg gtgtgaagac ccactgccca ccctgcaacc
     4981 caggcttctt caaaaccaac aacagcacct gccagccctg cccatatggt tcctactcca
     5041 atggctcaga ctgtacccgc tgccctgcag ggactgaacc tgctgtggga tttgaataca
     5101 aatggtggaa cacgctgccc acaaacatgg aaacgaccgt tctcagtggg atcaacttcg
     5161 agtacaaggg catgacaggc tgggaggtgg ctggtgatca catttacaca gctgctggag
     5221 cctcagacaa tgacttcatg attctcactc tggttgtgcc aggatttaga cctccgcagt
     5281 cggtgatggc agacacagag aataaagagg tggccagaat cacatttgtc tttgagaccc
     5341 tctgttctgt gaactgtgag ctctacttca tggtgggtgt gaattctagg accaacactc
     5401 ctgtggagac gtggaaaggt tccaaaggca aacagtccta tacctacatc attgaggaga
     5461 acactaccac gagcttcacc tgggccttcc agaggaccac ttttcatgag gcaagcagga
     5521 agtacaccaa tgacgttgcc aagatctact ccatcaatgt caccaatgtt atgaatggtg
     5581 tggcctccta ctgccgtccc tgtgccctag aagcctctga tgtgggctcc tcctgcacct
     5641 cttgtcctgc tggttactat attgaccgag attcaggaac ctgccactcc tgccccacta
     5701 acacaattct gaaagcccac cagccttatg gtgtccaggc ctgtgtgccc tgtggtccag
     5761 ggaccaagaa caacaagatc cactctctgt gctacaacga ttgcaccttc tcacgcaaca
     5821 ctccgaccag gactttcaac tacaacttct ccgctttggc aaacactgtc actcttgctg
     5881 gagggccaag cttcacttcc aaagggctga aatacttcca tcactttacc ctcagtctct
     5941 gtggaaacca gggtaggaaa atgtctgtgt gcaccgacaa tgtcactgac ctccggattc
     6001 ctgagggtga gtcagggttc tccaaatcta tcacagccta cgtctgccag gcagtcatca
     6061 tccccccaga ggtgacaggc tacaaggccg gggtttcctc acagcctgtc agccttgctg
     6121 atcgacttat tggggtgaca acagatatga ctctggatgg aatcacctcc ccagctgaac
     6181 ttttccacct ggagtccttg ggaataccgg acgtgatctt cttttatagg tccaatgatg
     6241 tgacccagtc ctgcagttct gggagatcaa ccaccatccg cgtcaggtgc agtccacaga
     6301 aaactgtccc tggaagtttg ctgctgccag gaacgtgctc ggatgggacc tgtgatggct
     6361 gcaacttcca cttcctgtgg gagagcgcgg ctgcttgccc gctctgctca gtggctgact
     6421 accatgctat cgtcagcagc tgtgtggctg ggatccagaa gactacttac gtgtggcgag
     6481 aacccaagct atgctctggt ggcatttctc tgcctgagca gagagtcacc atctgcaaaa
     6541 ccatagattt ctggctgaaa gtgggcatct ctgcaggcac ctgtactgcc atcctgctca
     6601 ccgtcttgac ctgctacttt tggaaaaaga atcaaaaact agagtacaag tactccaagc
     6661 tggtgatgaa tgctactctc aaggactgtg acctgccagc agctgacagc tgcgccatca
     6721 tggaaggcga ggatgtagag gacgacctca tctttaccag caagaagtca ctctttggga
     6781 agatcaaatc atttacctcc aagaggactc ctgatggatt tgactcagtg ccgctgaaga
     6841 catcctcagg aggcctagac atggacctgg atatcctgca gctgcctccc ctggagcgcc
     6901 tgaccctgga ctaagaccca gctttcttgt acaaagtggt tggtaagcct atccctaacc
     6961 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
     7021 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     7081 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     7141 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     7201 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     7261 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     7321 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     7381 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     7441 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     7501 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     7561 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact
     7621 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     7681 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     7741 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7801 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7861 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     7921 acgtatagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     7981 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     8041 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     8101 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     8161 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     8221 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     8281 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     8341 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     8401 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     8461 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     8521 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     8581 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     8641 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     8701 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     8761 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     8821 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     8881 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     8941 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     9001 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     9061 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     9121 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     9181 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     9241 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     9301 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     9361 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     9421 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     9481 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     9541 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     9601 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     9661 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     9721 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     9781 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     9841 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     9901 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     9961 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
    10021 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
    10081 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
    10141 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
    10201 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
    10261 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
    10321 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
    10381 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
    10441 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    10501 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    10561 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    10621 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    10681 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
    10741 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
    10801 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgc
//
