LOCUS       40924_29406        4049 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLX-R3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez 
            D, Garcia JA.
  TITLE     Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors 
            with Compatible Replication Origins
  JOURNAL   ACS Synth Biol (2017) In press
  PUBMED    28657330
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Pasin F, Garcia JA.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2017) Department of Plant Molecular Genetics, 
            Centro Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 4049)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4049)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-MAR-2017) Department of Plant Molecular Genetics, Centro 
            Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, Madrid 
            28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..24
                     /label=assembly linker
                     /note="assembly linker"
     regulatory      25..72
                     /regulatory_class="terminator"
     misc_feature    133..157
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    184..208
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(308..478)
                     /label=lacZ-alpha
                     /note="LacZ-alpha fragment of beta-galactosidase"
     promoter        complement(527..557)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     misc_feature    653..676
                     /label=RB
                     /note="RB"
     regulatory      complement(741..781)
                     /regulatory_class="terminator"
     misc_feature    791..814
                     /label=assembly linker
                     /note="assembly linker"
     CDS             complement(818..1606)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     misc_feature    1804..1827
                     /label=assembly linker
                     /note="assembly linker"
     CDS             complement(1881..3026)
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     rep_origin      complement(3419..3950)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 ccatcatcag ttcggtggtc ttccgacgaa caataaggcc gcaaatcgcg gcctttttta
       61 ttgataacaa aaccggctca gttctgcgta gaaaccaaca tgcaagctcc accgggtgca
      121 aagcggcagc ggcggcagga tatattcaat tgtaaatggc ttcatgtccg ggaaatctac
      181 tggtggcagg atatattgtg gtgtaaacaa tggagaaaaa gattaattaa gtactagtag
      241 cctggtcgaa accgtctcag gagagagacc aaaagcaaaa acccgccgaa gcgggttact
      301 agcgccattc gccattcaga gagcggagct gctgcgacgg acgatcggta cgcgcctctt
      361 cgctattacg ccaactggcg aaaggtggat gtgctgcaag gcgattaagt tgggtaacgc
      421 cagggttttc ccagtcacga cgttgtagta ccacggcaag gctatctgta atcattgttg
      481 tactccggtt aggacggatt gggaactggc taactcaaaa tccacacatt atacgagccg
      541 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag gtctctcgct gtcaggagac
      601 gggacaagga tgcgagctag cctgcaggaa ttgttgattt tgtgatgact gatggcagga
      661 tatatgcggt tgtaattcat ttttattgtc taaatttctg tatttgtttg tttgttcggt
      721 tgtaaatttt tttggaagaa caagaaaaga aaaaacaccc gttagggtgt ttttagttag
      781 tgtggcgcgc cgacttgcga catgcggtcc tttgttattt gccgactacc ttggtgatct
      841 cgcctttcac gtagtggaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt
      901 cttcttgtcc aagataagcc tgtctagctt caagtatgac gggctgatac tgggccggca
      961 ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc
     1021 tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg
     1081 gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg
     1141 gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg
     1201 tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct gcaagaatgt
     1261 cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga
     1321 tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc tctccagggg
     1381 aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta
     1441 cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca tccactgcgg
     1501 agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta
     1561 cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcatgatg tttaactttg
     1621 ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac
     1681 ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtacc ccaaaaaaac
     1741 agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg
     1801 ttcgatcgga ttggcggtta tgcggttgcg atgcaggtgg ctgctgaacc cccagccgga
     1861 actgacccca caaggcccta gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt
     1921 tccaccaggc cgctgcctcg caactcttcg caggcttcgc cgacctgctc gcgccacttc
     1981 ttcacgcggg tggaatccga tccgcacatg aggcggaagg tttccagctt gagcgggtac
     2041 ggctcccggt gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg
     2101 tacttctccc atatgaattt cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg
     2161 tcgatcagga cctggcaacg ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc
     2221 agcagcgaca ccgattccag gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc
     2281 tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc ggccattgat cgaccagccc
     2341 aggtcctggc aaagctcgta gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc
     2401 gcgtactcca acacctgctg ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg
     2461 gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga ccttgttttg cagcgcctcg
     2521 cgcgggattt tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc gtttggcatc
     2581 gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg aaagctgcat ttccttgatc
     2641 tgctgcttcg tgtgtttcag caacgcggcc tgcttggctt cgctgacctg ttttgccagg
     2701 tcctcgccgg cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc
     2761 gacttcgcca aacctgccgc ctcctgttcg agacgacgcg aacgctccac ggcggccgat
     2821 ggcgcgggca gggcaggggg agccagttgc acgctgtcgc gctcgatctt ggccgtagct
     2881 tgctggacta tcgagccgac ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg
     2941 cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat
     3001 gccttccggt caaacgtccg attcattcac cctccttgcg ggattgcccc ggaattaatt
     3061 ccccggatcg atccgtcgat cttgatcccc tgcgccatca gatccttggc ggcaagaaag
     3121 ccatccagtt tactttgcag ggcttcccaa ccttaccaga gggcgcccca gctggcaatt
     3181 ccggttcgct tgctgtccat aaaaccgccc agtctagcta tcgccatgta agcccactgc
     3241 aagctacctg ctttctcttt gcgcttgcgt tttcccttgt ccagatagcc cagtagctga
     3301 cattcatccg gggtcagcac cgtttctgcg gactggcttt ctacgtggct gccatttttg
     3361 gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg ggatgggagg cccgcgttag
     3421 cgggccggga gggttcgaga agggggggca ccccccttcg gcgtgcgcgg tcacgcgcac
     3481 agggcgcagc cctggttaaa aacaaggttt ataaatattg gtttaaaagc aggttaaaag
     3541 acaggttagc ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga ttttctgcct
     3601 gtggacagcc cctcaaatgt caataggtgc gcccctcatc tgtcagcact ctgcccctca
     3661 agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg tcaataccgc
     3721 agggcactta tccccaggct tgtccacatc atctgtggga aactcgcgta aaatcaggcg
     3781 ttttcgccga tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg agcctgcccc
     3841 tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc cgcccctcat
     3901 ctgtcagtga gggccaagtt ttccgcgagg tatccacaac gccggcggcc ctacatggct
     3961 ctgctgtagt gagtgggttg cgctccggca gcggtcctga tcccccgcag aaaaaaagga
     4021 tctcaagaag atcctttgat cttttctac
//
