LOCUS       40924_29396        3987 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLX-R2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez 
            D, Garcia JA.
  TITLE     Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors 
            with Compatible Replication Origins
  JOURNAL   ACS Synth Biol (2017) In press
  PUBMED    28657330
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Pasin F, Garcia JA.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2017) Department of Plant Molecular Genetics, 
            Centro Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 3987)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3987)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-MAR-2017) Department of Plant Molecular Genetics, Centro 
            Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, Madrid 
            28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3987
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..24
                     /label=assembly linker
                     /note="assembly linker"
     regulatory      25..72
                     /regulatory_class="terminator"
     misc_feature    133..157
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    184..208
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(308..478)
                     /label=lacZ-alpha
                     /note="LacZ-alpha fragment of beta-galactosidase"
     promoter        complement(527..557)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     misc_feature    653..676
                     /label=RB
                     /note="RB"
     regulatory      complement(741..781)
                     /regulatory_class="terminator"
     misc_feature    791..814
                     /label=assembly linker
                     /note="assembly linker"
     CDS             complement(828..1640)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    1742..1765
                     /label=assembly linker
                     /note="assembly linker"
     CDS             complement(1819..2964)
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     rep_origin      complement(3357..3888)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 ccatcatcag ttcggtggtc ttccgacgaa caataaggcc gcaaatcgcg gcctttttta
       61 ttgataacaa aaccggctca gttctgcgta gaaaccaaca tgcaagctcc accgggtgca
      121 aagcggcagc ggcggcagga tatattcaat tgtaaatggc ttcatgtccg ggaaatctac
      181 tggtggcagg atatattgtg gtgtaaacaa tggagaaaaa gattaattaa gtactagtag
      241 cctggtcgaa accgtctcag gagagagacc aaaagcaaaa acccgccgaa gcgggttact
      301 agcgccattc gccattcaga gagcggagct gctgcgacgg acgatcggta cgcgcctctt
      361 cgctattacg ccaactggcg aaaggtggat gtgctgcaag gcgattaagt tgggtaacgc
      421 cagggttttc ccagtcacga cgttgtagta ccacggcaag gctatctgta atcattgttg
      481 tactccggtt aggacggatt gggaactggc taactcaaaa tccacacatt atacgagccg
      541 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag gtctctcgct gtcaggagac
      601 gggacaagga tgcgagctag cctgcaggaa ttgttgattt tgtgatgact gatggcagga
      661 tatatgcggt tgtaattcat ttttattgtc taaatttctg tatttgtttg tttgttcggt
      721 tgtaaatttt tttggaagaa caagaaaaga aaaaacaccc gttagggtgt ttttagttag
      781 tgtggcgcgc cgacttgcga catgcggtcc tttgcaatca actattagaa aaattcatcc
      841 agcatcagat gaaattgcag tttgttcata tccggattat caatgccata tttctgaaac
      901 agacgttttt gcaggctcgg gctaaattcg cccaggcagt tccacagaat ggccagatcc
      961 tgataacgat ccgcaatgcc cacacggccc acatcaatgc agccaatcag tttgccttca
     1021 tcgaaaatca ggttatccag gctaaaatcg ccgtgggtca ccacgctatc cgggctaaac
     1081 ggcagcagtt tatgcatttc tttccacacc tgttccaccg gccagccgtt acgttcatca
     1141 tcaaaatcgc tcgcatccac caggccgttg ttcatacggc tctgcgcctg ggccagacga
     1201 aacacacgat cgctgttaaa cgggcagttg cacaccggaa tgctatgcag acgacgcaga
     1261 aacacggcca gcgcatccac aatgttttcg ccgctatccg gatattcttc cagcacctga
     1321 aacgcggttt tgcccggaat cgcggtggtc agcagccacg catcatccgg ggtgcgaata
     1381 aaatgtttaa tggtcggcag cggcataaat tcggtcagcc agttcagacg caccatttca
     1441 tcggtcacat cgttcgccac gctgcctttg ccatgtttca gaaacagttc cggcgcatcc
     1501 ggtttgccat acagacgata aatggtcgcg ccgctctgac ccacgttatc acgcgcccat
     1561 ttatagccat acagatccgc atccatgttg ctgttcagac gcggacggct acagctcgtt
     1621 tcacgctgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt
     1681 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca
     1741 agatcggatt ggcggttatg cggttgcgat gcaggtggct gctgaacccc cagccggaac
     1801 tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc aggcgtgttc
     1861 caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc gccacttctt
     1921 cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga gcgggtacgg
     1981 ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta
     2041 cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga tttcctcgtc
     2101 gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga agcggtgcag
     2161 cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg
     2221 taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg accagcccag
     2281 gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc
     2341 gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct cgacgccggt
     2401 gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg
     2461 cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc
     2521 tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt ccttgatctg
     2581 ctgcttcgtg tgtttcagca acgcggcctg cttggcttcg ctgacctgtt ttgccaggtc
     2641 ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga tggtcatcga
     2701 cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa cgctccacgg cggccgatgg
     2761 cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg
     2821 ctggactatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga cggtgcggct
     2881 tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc
     2941 cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccgg aattaattcc
     3001 ccggatcgat ccgtcgatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc
     3061 atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc
     3121 ggttcgcttg ctgtccataa aaccgcccag tctagctatc gccatgtaag cccactgcaa
     3181 gctacctgct ttctctttgc gcttgcgttt tcccttgtcc agatagccca gtagctgaca
     3241 ttcatccggg gtcagcaccg tttctgcgga ctggctttct acgtggctgc catttttggg
     3301 gtgaggccgt tcgcggccga ggggcgcagc ccctgggggg atgggaggcc cgcgttagcg
     3361 ggccgggagg gttcgagaag ggggggcacc ccccttcggc gtgcgcggtc acgcgcacag
     3421 ggcgcagccc tggttaaaaa caaggtttat aaatattggt ttaaaagcag gttaaaagac
     3481 aggttagcgg tggccgaaaa acgggcggaa acccttgcaa atgctggatt ttctgcctgt
     3541 ggacagcccc tcaaatgtca ataggtgcgc ccctcatctg tcagcactct gcccctcaag
     3601 tgtcaaggat cgcgcccctc atctgtcagt agtcgcgccc ctcaagtgtc aataccgcag
     3661 ggcacttatc cccaggcttg tccacatcat ctgtgggaaa ctcgcgtaaa atcaggcgtt
     3721 ttcgccgatt tgcgaggctg gccagctcca cgtcgccggc cgaaatcgag cctgcccctc
     3781 atctgtcaac gccgcgccgg gtgagtcggc ccctcaagtg tcaacgtccg cccctcatct
     3841 gtcagtgagg gccaagtttt ccgcgaggta tccacaacgc cggcggccct acatggctct
     3901 gctgtagtga gtgggttgcg ctccggcagc ggtcctgatc ccccgcagaa aaaaaggatc
     3961 tcaagaagat cctttgatct tttctac
//
