LOCUS       40924_29376        3165 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLX-B4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez 
            D, Garcia JA.
  TITLE     Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors 
            with Compatible Replication Origins
  JOURNAL   ACS Synth Biol (2017) In press
  PUBMED    28657330
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Pasin F, Garcia JA.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2017) Department of Plant Molecular Genetics, 
            Centro Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 3165)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3165)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-MAR-2017) Department of Plant Molecular Genetics, Centro 
            Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, Madrid 
            28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..24
                     /label=assembly linker
                     /note="assembly linker"
     regulatory      25..72
                     /regulatory_class="terminator"
     misc_feature    133..157
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    184..208
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(308..478)
                     /label=lacZ-alpha
                     /note="LacZ-alpha fragment of beta-galactosidase"
     promoter        complement(527..557)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     misc_feature    653..676
                     /label=RB
                     /note="RB"
     regulatory      complement(741..781)
                     /regulatory_class="terminator"
     misc_feature    791..814
                     /label=assembly linker
                     /note="assembly linker"
     CDS             complement(872..1402)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(1591..1619)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     misc_feature    1620..1643
                     /label=assembly linker
                     /note="assembly linker"
     CDS             complement(1647..2306)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(2307..3078)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 ccatcatcag ttcggtggtc ttccgacgaa caataaggcc gcaaatcgcg gcctttttta
       61 ttgataacaa aaccggctca gttctgcgta gaaaccaaca tgcaagctcc accgggtgca
      121 aagcggcagc ggcggcagga tatattcaat tgtaaatggc ttcatgtccg ggaaatctac
      181 tggtggcagg atatattgtg gtgtaaacaa tggagaaaaa gattaattaa gtactagtag
      241 cctggtcgaa accgtctcag gagagagacc aaaagcaaaa acccgccgaa gcgggttact
      301 agcgccattc gccattcaga gagcggagct gctgcgacgg acgatcggta cgcgcctctt
      361 cgctattacg ccaactggcg aaaggtggat gtgctgcaag gcgattaagt tgggtaacgc
      421 cagggttttc ccagtcacga cgttgtagta ccacggcaag gctatctgta atcattgttg
      481 tactccggtt aggacggatt gggaactggc taactcaaaa tccacacatt atacgagccg
      541 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag gtctctcgct gtcaggagac
      601 gggacaagga tgcgagctag cctgcaggaa ttgttgattt tgtgatgact gatggcagga
      661 tatatgcggt tgtaattcat ttttattgtc taaatttctg tatttgtttg tttgttcggt
      721 tgtaaatttt tttggaagaa caagaaaaga aaaaacaccc gttagggtgt ttttagttag
      781 tgtggcgcgc cgacttgcga catgcggtcc tttgcaattt acccaacaac tccgcggccg
      841 ggaagccgat ctcggcttga acgaattgtt aggtggcggt acttgggtcg atatcaaagt
      901 gcatcacttc ttcccgtatg cccaactttg tatagagagc cactgcggga tcgtcaccgt
      961 aatctgcttg cacgtagatc acataagcac caagcgcgtt ggcctcatgc ttgaggagat
     1021 tgatgagcgc ggtggcaatg ccctgcctcc ggtgctctcc ggagactgcg agatcataga
     1081 tatagatctc actacgcggc tgctcaaact tgggcagaac gtaagccgcg agagcgccaa
     1141 caaccgcttc ttggtcgaag gcagcaagcg cgatgaatgt cttactacgg agcaagttcc
     1201 cgaggtaatc ggagtccggc tgatgttggg agtaggtggc tacgtctccg aactcacgac
     1261 cgaaaagatc aagagcagcc cgcatggatt tgacttggtc agggccgagc ctacatgtgc
     1321 gaatgatgcc catacttgag ccacctaact ttgttttagg gcgactgccc tgctgcgtaa
     1381 catcgttgct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg
     1441 taacgcgctt gctgcttgga tgcccgaggc ataggctgta caaaaaaaca gtcataacaa
     1501 gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag
     1561 ttgcgtgagc gcatacgcta cttgcattac agtttacgaa ccgaacaggc ttatgtcaag
     1621 atcggattgg cggttatgcg gttctaccgg cgcggcagcg ttacccgtgt cggcggctcc
     1681 aacggctcgc catcgtccag aaaacacggc tcatcgggca tcggcaggcg ctgctgcccg
     1741 cgccgttccc attcctccgt ttcggtcaag gctggcaggt ctggttccat gcccggaatg
     1801 ccgggctggc tgggcggctc ctcgccgggg ccggtcggta gttgctgctc gcccggatac
     1861 agggtcggga tgcggcgcag gtcgccatgc cccaacagcg attcgtcctg gtcgtcgtga
     1921 tcaaccacca cggcggcact gaacaccgac aggcgcaact ggtcgcgggg ctggccccac
     1981 gccacgcggt cattgaccac gtaggccgac acggtgccgg ggccgttgag cttcacgacg
     2041 gagatccagc gctcggccac caagtccttg actgcgtatt ggaccgtccg caaagaacgt
     2101 ccgatgagct tggaaagtgt cttctggctg accaccacgg cgttctggtg gcccatctgc
     2161 gccacgaggt gatgcagcag cattgccgcc gtgggtttcc tcgcaataag cccggcccac
     2221 gcctcatgcg ctttgcgttc cgtttgcacc cagtgaccgg gcttgttctt ggcttgaatg
     2281 ccgatttctc tggactgcgt ggccatgctt atctccatgc ggtaggggtg ccgcacggtt
     2341 gcggcaccat gcgcaatcag ctgcaacttt tcggcagcgc gacaacaatt atgcgttgcg
     2401 taaaagtggc agtcaattac agattttctt taacctacgc aatgagctat tgcggggggt
     2461 gccgcaatga gctgttgcgt accccccttt tttaagttgt tgatttttaa gtctttcgca
     2521 tttcgcccta tatctagttc tttggtgccc aaagaagggc acccctgcgg ggttccccca
     2581 cgccttcggc gcggctcccc ctccggcaaa aagtggcccc tccggggctt gttgatcgac
     2641 tgcgcggcct tcggccttgc ccaaggtggc gctgccccct tggaaccccc gcactcgccg
     2701 ccgtgaggct cggggggcag gcgggcgggc ttcgcccttc gactgccccc actcgcatag
     2761 gcttgggtcg ttccaggcgc gtcaaggcca agccgctgcg cggtcgctgc gcgagccttg
     2821 acccgccttc cacttggtgt ccaaccggca agcgaagcgc gcaggccgca ggccggaggc
     2881 ttttccccag agaaaattaa aaaaattgat ggggcaaggc cgcaggccgc gcagttggag
     2941 ccggtgggta tgtggtcgaa ggctgggtag ccggtgggca atccctgtgg tcaagctcgt
     3001 gggcaggcgc agcctgtcca tcagcttgtc cagcagggtt gtccacgggc cgagcgaagc
     3061 gagccagccg gtggccgctc gcggccatcg tccacatatc cacgggctgg caagggagcg
     3121 cagcgaccgc gcagggcgaa gcccggagag caagcccgta ggggg
//
