LOCUS       40924_29356        3349 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLX-B3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez 
            D, Garcia JA.
  TITLE     Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors 
            with Compatible Replication Origins
  JOURNAL   ACS Synth Biol (2017) In press
  PUBMED    28657330
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Pasin F, Garcia JA.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2017) Department of Plant Molecular Genetics, 
            Centro Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 3349)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3349)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-MAR-2017) Department of Plant Molecular Genetics, Centro 
            Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, Madrid 
            28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..24
                     /label=assembly linker
                     /note="assembly linker"
     regulatory      25..72
                     /regulatory_class="terminator"
     misc_feature    133..157
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    184..208
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(308..478)
                     /label=lacZ-alpha
                     /note="LacZ-alpha fragment of beta-galactosidase"
     promoter        complement(527..557)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     misc_feature    653..676
                     /label=RB
                     /note="RB"
     regulatory      complement(741..781)
                     /regulatory_class="terminator"
     misc_feature    791..814
                     /label=assembly linker
                     /note="assembly linker"
     CDS             complement(818..1606)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     misc_feature    1804..1827
                     /label=assembly linker
                     /note="assembly linker"
     CDS             complement(1831..2490)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(2491..3262)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 ccatcatcag ttcggtggtc ttccgacgaa caataaggcc gcaaatcgcg gcctttttta
       61 ttgataacaa aaccggctca gttctgcgta gaaaccaaca tgcaagctcc accgggtgca
      121 aagcggcagc ggcggcagga tatattcaat tgtaaatggc ttcatgtccg ggaaatctac
      181 tggtggcagg atatattgtg gtgtaaacaa tggagaaaaa gattaattaa gtactagtag
      241 cctggtcgaa accgtctcag gagagagacc aaaagcaaaa acccgccgaa gcgggttact
      301 agcgccattc gccattcaga gagcggagct gctgcgacgg acgatcggta cgcgcctctt
      361 cgctattacg ccaactggcg aaaggtggat gtgctgcaag gcgattaagt tgggtaacgc
      421 cagggttttc ccagtcacga cgttgtagta ccacggcaag gctatctgta atcattgttg
      481 tactccggtt aggacggatt gggaactggc taactcaaaa tccacacatt atacgagccg
      541 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag gtctctcgct gtcaggagac
      601 gggacaagga tgcgagctag cctgcaggaa ttgttgattt tgtgatgact gatggcagga
      661 tatatgcggt tgtaattcat ttttattgtc taaatttctg tatttgtttg tttgttcggt
      721 tgtaaatttt tttggaagaa caagaaaaga aaaaacaccc gttagggtgt ttttagttag
      781 tgtggcgcgc cgacttgcga catgcggtcc tttgttattt gccgactacc ttggtgatct
      841 cgcctttcac gtagtggaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt
      901 cttcttgtcc aagataagcc tgtctagctt caagtatgac gggctgatac tgggccggca
      961 ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc
     1021 tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg
     1081 gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg
     1141 gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg
     1201 tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct gcaagaatgt
     1261 cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga
     1321 tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc tctccagggg
     1381 aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta
     1441 cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca tccactgcgg
     1501 agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta
     1561 cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcatgatg tttaactttg
     1621 ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac
     1681 ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtacc ccaaaaaaac
     1741 agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg
     1801 ttcgatcgga ttggcggtta tgcggttcta ccggcgcggc agcgttaccc gtgtcggcgg
     1861 ctccaacggc tcgccatcgt ccagaaaaca cggctcatcg ggcatcggca ggcgctgctg
     1921 cccgcgccgt tcccattcct ccgtttcggt caaggctggc aggtctggtt ccatgcccgg
     1981 aatgccgggc tggctgggcg gctcctcgcc ggggccggtc ggtagttgct gctcgcccgg
     2041 atacagggtc gggatgcggc gcaggtcgcc atgccccaac agcgattcgt cctggtcgtc
     2101 gtgatcaacc accacggcgg cactgaacac cgacaggcgc aactggtcgc ggggctggcc
     2161 ccacgccacg cggtcattga ccacgtaggc cgacacggtg ccggggccgt tgagcttcac
     2221 gacggagatc cagcgctcgg ccaccaagtc cttgactgcg tattggaccg tccgcaaaga
     2281 acgtccgatg agcttggaaa gtgtcttctg gctgaccacc acggcgttct ggtggcccat
     2341 ctgcgccacg aggtgatgca gcagcattgc cgccgtgggt ttcctcgcaa taagcccggc
     2401 ccacgcctca tgcgctttgc gttccgtttg cacccagtga ccgggcttgt tcttggcttg
     2461 aatgccgatt tctctggact gcgtggccat gcttatctcc atgcggtagg ggtgccgcac
     2521 ggttgcggca ccatgcgcaa tcagctgcaa cttttcggca gcgcgacaac aattatgcgt
     2581 tgcgtaaaag tggcagtcaa ttacagattt tctttaacct acgcaatgag ctattgcggg
     2641 gggtgccgca atgagctgtt gcgtaccccc cttttttaag ttgttgattt ttaagtcttt
     2701 cgcatttcgc cctatatcta gttctttggt gcccaaagaa gggcacccct gcggggttcc
     2761 cccacgcctt cggcgcggct ccccctccgg caaaaagtgg cccctccggg gcttgttgat
     2821 cgactgcgcg gccttcggcc ttgcccaagg tggcgctgcc cccttggaac ccccgcactc
     2881 gccgccgtga ggctcggggg gcaggcgggc gggcttcgcc cttcgactgc ccccactcgc
     2941 ataggcttgg gtcgttccag gcgcgtcaag gccaagccgc tgcgcggtcg ctgcgcgagc
     3001 cttgacccgc cttccacttg gtgtccaacc ggcaagcgaa gcgcgcaggc cgcaggccgg
     3061 aggcttttcc ccagagaaaa ttaaaaaaat tgatggggca aggccgcagg ccgcgcagtt
     3121 ggagccggtg ggtatgtggt cgaaggctgg gtagccggtg ggcaatccct gtggtcaagc
     3181 tcgtgggcag gcgcagcctg tccatcagct tgtccagcag ggttgtccac gggccgagcg
     3241 aagcgagcca gccggtggcc gctcgcggcc atcgtccaca tatccacggg ctggcaaggg
     3301 agcgcagcga ccgcgcaggg cgaagcccgg agagcaagcc cgtaggggg
//
