LOCUS       40924_29336       10749 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLX-B2-XT1-XT2-hCas9, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10749)
  AUTHORS   Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez 
            D, Garcia JA.
  TITLE     Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors 
            with Compatible Replication Origins
  JOURNAL   ACS Synth Biol (2017) In press
  PUBMED    28657330
REFERENCE   2  (bases 1 to 10749)
  AUTHORS   Pasin F, Garcia JA.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2017) Department of Plant Molecular Genetics, 
            Centro Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 10749)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10749)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-MAR-2017) Department of Plant Molecular Genetics, Centro 
            Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, Madrid 
            28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..24
                     /label=assembly linker
                     /note="assembly linker"
     regulatory      25..72
                     /regulatory_class="terminator"
     misc_feature    133..157
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    184..208
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(287..539)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(582..1373)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(1407..1586)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     regulatory      1677..1878
                     /label=U626 (Arabidopsis thaliana)
                     /note="U626 (Arabidopsis thaliana)"
                     /regulatory_class="promoter"
     misc_RNA        1902..1977
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(1989..2013)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2031..2232
                     /label=U626 (Arabidopsis thaliana)
                     /note="U626 (Arabidopsis thaliana)"
                     /regulatory_class="promoter"
     misc_RNA        2256..2338
                     /label=sgRNA
                     /note="sgRNA"
     misc_RNA        2256..2331
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(2343..2367)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2996..3340
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3413..7516
                     /label=Cas9
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
     misc_feature    7529..7549
                     /label=SV40 NLS
                     /note="SV40 NLS"
     CDS             7529..7549
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7576..7828
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    8115..8138
                     /label=RB
                     /note="RB"
     regulatory      complement(8203..8243)
                     /regulatory_class="terminator"
     misc_feature    8253..8276
                     /label=assembly linker
                     /note="assembly linker"
     CDS             complement(8290..9102)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    9204..9227
                     /label=assembly linker
                     /note="assembly linker"
     CDS             complement(9231..9890)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(9891..10662)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 ccatcatcag ttcggtggtc ttccgacgaa caataaggcc gcaaatcgcg gcctttttta
       61 ttgataacaa aaccggctca gttctgcgta gaaaccaaca tgcaagctcc accgggtgca
      121 aagcggcagc ggcggcagga tatattcaat tgtaaatggc ttcatgtccg ggaaatctac
      181 tggtggcagg atatattgtg gtgtaaacaa tggagaaaaa gattaattaa gtactagtag
      241 cctggtcgaa accgtctcag gagagcgatc agcttgcatg ccggtcgatc tagtaacata
      301 gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt ttctatcgcg
      361 tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa taacgtcatg
      421 cattacatgt taattattac atgcttaacg taattcaaca gaaattatat gataatcatg
      481 gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatct
      541 gcttgactct agctagagtc cgaaccccag agtcccgctc agaagaactc gtcaagaagg
      601 cgatagaagg ctatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
      661 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
      721 tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
      781 accatgatat tcggcaagca ggcgtcgccg tgggtcacga cgagatcctc gccgtcgggc
      841 atccgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
      901 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcggtgt
      961 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
     1021 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc
     1081 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct
     1141 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttggagttca
     1201 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc
     1261 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc
     1321 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcctcga
     1381 tcgagttgag agtgaatatg agactctaat tggataccga ggggaattta tggaacgtca
     1441 gtggagcatt tttgacaaga aatatttgct agctgatagt gaccttaggc gacttttgaa
     1501 cgcgcaataa tggtttctga cgtatgtgct tagctcatta aactccagaa acccgcggct
     1561 gagtggctcc ttcaacgttg cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg
     1621 tcatcggcgg gggtcataac gtgactccct taattctcat gtatctccgt caggagcatc
     1681 ttcattctta agatatgaag ataatcttca aaaggcccct gggaatctga aagaagagaa
     1741 gcaggcccat ttatatggga aagaacaata gtatttctta tataggccca tttaagttga
     1801 aaacaatctt caaaagtccc acatcgctta gataagaaaa cgaagctgag tttatataca
     1861 gctagagtcg aagtagtgat tgaaaacacc gtcttcggag agttttagag ctagaaatag
     1921 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
     1981 ttttctagac ccagctttct tgtacaaagt tggcattacg ctgtcaggag catcttcatt
     2041 cttaagatat gaagataatc ttcaaaaggc ccctgggaat ctgaaagaag agaagcaggc
     2101 ccatttatat gggaaagaac aatagtattt cttatatagg cccatttaag ttgaaaacaa
     2161 tcttcaaaag tcccacatcg cttagataag aaaacgaagc tgagtttata tacagctaga
     2221 gtcgaagtag tgattgaaaa ttgggaaaaa actaggtttt agagctagaa atagcaagtt
     2281 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttct
     2341 agacccagct ttcttgtaca aagttggcat tacgctgtca ggagactaga gccaagctga
     2401 tctcctttgc cccggagatc accatggacg actttctcta tctctacgat ctaggaagaa
     2461 agttcgacgg agaaggtgac gataccatgt tcaccaccga taatgagaag attagcctct
     2521 tcaatttcag aaagaatgct gacccacaga tggttagaga ggcctacgcg gcaggtctga
     2581 tcaagacgat ctacccgagt aataatctcc aggagatcaa ataccttccc aagaaggtta
     2641 aagatgcagt caaaagattc aggactaact gcatcaagaa cacagagaaa gatatatttc
     2701 tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcat aaaccaaggc
     2761 aagtaataga gattggagtc tctaagaaag tagttcctac tgaatcaaag gccatggagt
     2821 caaaaattca gatcgaggat ctaacagaac tcgccgtgaa gactggcgaa cagttcatac
     2881 agagtctttt acgactcaat gacaagaaga aaatcttcgt caacatggtg gagcacgaca
     2941 ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccaaagg gctattgaga
     3001 cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc
     3061 acttcatcaa aaggacagta gaaaaggaag gtggcaccta caaatgccat cattgcgata
     3121 aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
     3181 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
     3241 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc
     3301 cttcctctat ataaggaagt tcatttcatt tggagaggac tccggtattt ttacaacaat
     3361 accacaacaa aacaaacaac aaacaacatt acaatttact attctagtcg aaatggacaa
     3421 gaagtactcc attgggctcg atatcggcac aaacagcgtc ggctgggccg tcattacgga
     3481 cgagtacaag gtgccgagca aaaaattcaa agttctgggc aataccgatc gccacagcat
     3541 aaagaagaac ctcattggcg ccctcctgtt cgactccggg gaaacggccg aagccacgcg
     3601 gctcaaaaga acagcacggc gcagatatac ccgcagaaag aatcggatct gctacctgca
     3661 ggagatcttt agtaatgaga tggctaaggt ggatgactct ttcttccata ggctggagga
     3721 gtcctttttg gtggaggagg ataaaaagca cgagcgccac ccaatctttg gcaatatcgt
     3781 ggacgaggtg gcgtaccatg aaaagtaccc aaccatatat catctgagga agaagcttgt
     3841 agacagtact gataaggctg acttgcggtt gatctatctc gcgctggcgc atatgatcaa
     3901 atttcgggga cacttcctca tcgaggggga cctgaaccca gacaacagcg atgtcgacaa
     3961 actctttatc caactggttc agacttacaa tcagcttttc gaagagaacc cgatcaacgc
     4021 atccggagtt gacgccaaag caatcctgag cgctaggctg tccaaatccc ggcggctcga
     4081 aaacctcatc gcacagctcc ctggggagaa gaagaacggc ctgtttggta atcttatcgc
     4141 cctgtcactc gggctgaccc ccaactttaa atctaacttc gacctggccg aagatgccaa
     4201 gcttcaactg agcaaagaca cctacgatga tgatctcgac aatctgctgg cccagatcgg
     4261 cgaccagtac gcagaccttt ttttggcggc aaagaacctg tcagacgcca ttctgctgag
     4321 tgatattctg cgagtgaaca cggagatcac caaagctccg ctgagcgcta gtatgatcaa
     4381 gcgctatgat gagcaccacc aagacttgac tttgctgaag gcccttgtca gacagcaact
     4441 gcctgagaag tacaaggaaa ttttcttcga tcagtctaaa aatggctacg ccggatacat
     4501 tgacggcgga gcaagccagg aggaatttta caaatttatt aagcccatct tggaaaaaat
     4561 ggacggcacc gaggagctgc tggtaaagct taacagagaa gatctgttgc gcaaacagcg
     4621 cactttcgac aatggaagca tcccccacca gattcacctg ggcgaactgc acgctatcct
     4681 caggcggcaa gaggatttct accccttttt gaaagataac agggaaaaga ttgagaaaat
     4741 cctcacattt cggataccct actatgtagg ccccctcgcc cggggaaatt ccagattcgc
     4801 gtggatgact cgcaaatcag aagagactat cactccctgg aacttcgagg aagtcgtgga
     4861 taagggggcc tctgcccagt ccttcatcga aaggatgact aactttgata aaaatctgcc
     4921 taacgaaaag gtgcttccta aacactctct gctgtacgag tacttcacag tttataacga
     4981 gctcaccaag gtcaaatacg tcacagaagg gatgagaaag ccagcattcc tgtctggaga
     5041 gcagaagaaa gctatcgtgg acctcctctt caagacgaac cggaaagtta ccgtgaaaca
     5101 gctcaaagaa gattatttca aaaagattga atgtttcgac tctgttgaaa tcagcggagt
     5161 ggaggatcgc ttcaacgcat ccctgggaac gtatcacgat ctcctgaaaa tcattaaaga
     5221 caaggacttc ctggacaatg aggagaacga ggacattctt gaggacattg tcctcaccct
     5281 tacgttgttt gaagataggg agatgattga agaacgcttg aaaacttacg ctcatctctt
     5341 cgacgacaaa gtcatgaaac agctcaagag gcgccgatat acaggatggg ggcggctgtc
     5401 aagaaaactg atcaatggga tccgagacaa gcagagtgga aagacaatcc tggattttct
     5461 taagtccgat ggatttgcca accggaactt catgcagttg atccatgatg actctctcac
     5521 ctttaaggag gacatccaga aagcacaagt ttctggccag ggggacagtc tccacgagca
     5581 catcgctaat cttgcaggta gcccagctat caaaaaggga atactgcaga ccgttaaggt
     5641 cgtggatgaa ctcgtcaaag taatgggaag gcataagccc gagaatatcg ttatcgagat
     5701 ggcccgagag aaccaaacta cccagaaggg acagaagaac agtagggaaa ggatgaagag
     5761 gattgaagag ggtataaaag aactggggtc ccaaatcctt aaggaacacc cagttgaaaa
     5821 cacccagctt cagaatgaga agctctacct gtactacctg cagaacggca gggacatgta
     5881 cgtggatcag gaactggaca tcaatcggct ctccgactac gacgtggatc atatcgtgcc
     5941 ccagtctttt ctcaaagatg attctattga taataaagtg ttgacaagat ccgataaaaa
     6001 tagagggaag agtgataacg tcccctcaga agaagttgtc aagaaaatga aaaattattg
     6061 gcggcagctg ctgaacgcca aactgatcac acaacggaag ttcgataatc tgactaaggc
     6121 tgaacgaggt ggcctgtctg agttggataa agccggcttc atcaaaaggc agcttgttga
     6181 gacacgccag atcaccaagc acgtggccca aattctcgat tcacgcatga acaccaagta
     6241 cgatgaaaat gacaaactga ttcgagaggt gaaagttatt actctgaagt ctaagctggt
     6301 ttcagatttc agaaaggact ttcagtttta taaggtgaga gagatcaaca attaccacca
     6361 tgcgcatgat gcctacctga atgcagtggt aggcactgca cttatcaaaa aatatcccaa
     6421 gcttgaatct gaatttgttt acggagacta taaagtgtac gatgttagga aaatgatcgc
     6481 aaagtctgag caggaaatag gcaaggccac cgctaagtac ttcttttaca gcaatattat
     6541 gaattttttc aagaccgaga ttacactggc caatggagag attcggaagc gaccacttat
     6601 cgaaacaaac ggagaaacag gagaaatcgt gtgggacaag ggtagggatt tcgcgacagt
     6661 ccggaaggtc ctgtccatgc cgcaggtgaa catcgttaaa aagaccgaag tacagaccgg
     6721 aggcttctcc aaggaaagta tcctcccgaa aaggaacagc gacaagctga tcgcacgcaa
     6781 aaaagattgg gaccccaaga aatacggcgg attcgattct cctacagtcg cttacagtgt
     6841 actggttgtg gccaaagtgg agaaagggaa gtctaaaaaa ctcaaaagcg tcaaggaact
     6901 gctgggcatc acaatcatgg agcgatcaag cttcgaaaaa aaccccatcg actttctcga
     6961 ggcgaaagga tataaagagg tcaaaaaaga cctcatcatt aagcttccca agtactctct
     7021 ctttgagctt gaaaacggcc ggaaacgaat gctcgctagt gcgggcgagc tgcagaaagg
     7081 taacgagctg gcactgccct ctaaatacgt taatttcttg tatctggcca gccactatga
     7141 aaagctcaaa ggatctcccg aagataatga gcagaagcag ctgttcgtgg aacaacacaa
     7201 acactacctt gatgagatca tcgagcaaat aagcgaattc tccaaaagag tgatcctcgc
     7261 cgacgctaac ctcgataagg tgctttctgc ttacaataag cacagggata agcccatcag
     7321 ggagcaggca gaaaacatta tccacttgtt tactctgacc aacttgggcg cgcctgcagc
     7381 cttcaagtac ttcgacacca ccatagacag aaagcggtac acctctacaa aggaggtcct
     7441 ggacgccaca ctgattcatc agtcaattac ggggctctat gaaacaagaa tcgacctctc
     7501 tcagctcggt ggagacagca gggctgaccc caagaagaag aggaaggtgt gagcttggaa
     7561 tggatcttcg atcccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
     7621 gttgccggtc ttgcgacgat tatcatataa tttctgttga attacgttaa gcatgtaata
     7681 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
     7741 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
     7801 cgcdcggtgt catctatgtt actagatcgg gaattgccaa gctaattctt gaagacgaaa
     7861 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     7921 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7981 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatggga
     8041 ccgactcgcg ctgtcaggag acgggacaag gatgcgagct agcctgcagg aattgttgat
     8101 tttgtgatga ctgatggcag gatatatgcg gttgtaattc atttttattg tctaaatttc
     8161 tgtatttgtt tgtttgttcg gttgtaaatt tttttggaag aacaagaaaa gaaaaaacac
     8221 ccgttagggt gtttttagtt agtgtggcgc gccgacttgc gacatgcggt cctttgcaat
     8281 caactattag aaaaattcat ccagcatcag atgaaattgc agtttgttca tatccggatt
     8341 atcaatgcca tatttctgaa acagacgttt ttgcaggctc gggctaaatt cgcccaggca
     8401 gttccacaga atggccagat cctgataacg atccgcaatg cccacacggc ccacatcaat
     8461 gcagccaatc agtttgcctt catcgaaaat caggttatcc aggctaaaat cgccgtgggt
     8521 caccacgcta tccgggctaa acggcagcag tttatgcatt tctttccaca cctgttccac
     8581 cggccagccg ttacgttcat catcaaaatc gctcgcatcc accaggccgt tgttcatacg
     8641 gctctgcgcc tgggccagac gaaacacacg atcgctgtta aacgggcagt tgcacaccgg
     8701 aatgctatgc agacgacgca gaaacacggc cagcgcatcc acaatgtttt cgccgctatc
     8761 cggatattct tccagcacct gaaacgcggt tttgcccgga atcgcggtgg tcagcagcca
     8821 cgcatcatcc ggggtgcgaa taaaatgttt aatggtcggc agcggcataa attcggtcag
     8881 ccagttcaga cgcaccattt catcggtcac atcgttcgcc acgctgcctt tgccatgttt
     8941 cagaaacagt tccggcgcat ccggtttgcc atacagacga taaatggtcg cgccgctctg
     9001 acccacgtta tcacgcgccc atttatagcc atacagatcc gcatccatgt tgctgttcag
     9061 acgcggacgg ctacagctcg tttcacgctg aatatggctc ataacacccc ttgtattact
     9121 gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta
     9181 acatcagaga ttttgagaca caagatcgga ttggcggtta tgcggttcta ccggcgcggc
     9241 agcgttaccc gtgtcggcgg ctccaacggc tcgccatcgt ccagaaaaca cggctcatcg
     9301 ggcatcggca ggcgctgctg cccgcgccgt tcccattcct ccgtttcggt caaggctggc
     9361 aggtctggtt ccatgcccgg aatgccgggc tggctgggcg gctcctcgcc ggggccggtc
     9421 ggtagttgct gctcgcccgg atacagggtc gggatgcggc gcaggtcgcc atgccccaac
     9481 agcgattcgt cctggtcgtc gtgatcaacc accacggcgg cactgaacac cgacaggcgc
     9541 aactggtcgc ggggctggcc ccacgccacg cggtcattga ccacgtaggc cgacacggtg
     9601 ccggggccgt tgagcttcac gacggagatc cagcgctcgg ccaccaagtc cttgactgcg
     9661 tattggaccg tccgcaaaga acgtccgatg agcttggaaa gtgtcttctg gctgaccacc
     9721 acggcgttct ggtggcccat ctgcgccacg aggtgatgca gcagcattgc cgccgtgggt
     9781 ttcctcgcaa taagcccggc ccacgcctca tgcgctttgc gttccgtttg cacccagtga
     9841 ccgggcttgt tcttggcttg aatgccgatt tctctggact gcgtggccat gcttatctcc
     9901 atgcggtagg ggtgccgcac ggttgcggca ccatgcgcaa tcagctgcaa cttttcggca
     9961 gcgcgacaac aattatgcgt tgcgtaaaag tggcagtcaa ttacagattt tctttaacct
    10021 acgcaatgag ctattgcggg gggtgccgca atgagctgtt gcgtaccccc cttttttaag
    10081 ttgttgattt ttaagtcttt cgcatttcgc cctatatcta gttctttggt gcccaaagaa
    10141 gggcacccct gcggggttcc cccacgcctt cggcgcggct ccccctccgg caaaaagtgg
    10201 cccctccggg gcttgttgat cgactgcgcg gccttcggcc ttgcccaagg tggcgctgcc
    10261 cccttggaac ccccgcactc gccgccgtga ggctcggggg gcaggcgggc gggcttcgcc
    10321 cttcgactgc ccccactcgc ataggcttgg gtcgttccag gcgcgtcaag gccaagccgc
    10381 tgcgcggtcg ctgcgcgagc cttgacccgc cttccacttg gtgtccaacc ggcaagcgaa
    10441 gcgcgcaggc cgcaggccgg aggcttttcc ccagagaaaa ttaaaaaaat tgatggggca
    10501 aggccgcagg ccgcgcagtt ggagccggtg ggtatgtggt cgaaggctgg gtagccggtg
    10561 ggcaatccct gtggtcaagc tcgtgggcag gcgcagcctg tccatcagct tgtccagcag
    10621 ggttgtccac gggccgagcg aagcgagcca gccggtggcc gctcgcggcc atcgtccaca
    10681 tatccacggg ctggcaaggg agcgcagcga ccgcgcaggg cgaagcccgg agagcaagcc
    10741 cgtaggggg
//
