LOCUS       V013550                 9254 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013550
VERSION     V013550
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2196..2436
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     promoter        2510..3691
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             3721..4413
                     /label="ZsGreen1"
                     /note="Zoanthus green fluorescent protein"
     CDS             4489..5085
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    5182..5767
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5971..6604
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6732..6748)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6756..6772)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6780..6810)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6825..6846)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7134..7722)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7896..8753)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8754..8858)
                     /label="AmpR promoter"
     polyA_signal    8906..9040
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatctgggc aggaagaggg cctatttccc atgattcctt
     2221 catatttgca tatacgatac aaggctgtta gagagataat tagaattaat ttgactgtaa
     2281 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
     2341 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
     2401 tcgatttctt ggctttatat atcttgtgga aaggacgagg atccgcctaa ggttaagtcg
     2461 ccctcgctcg agcgagggcg acttaacctt aggttttttg aattctaccg ctccggtgcc
     2521 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     2581 aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     2641 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     2701 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc
     2761 cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacgccc
     2821 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
     2881 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcttgggcg
     2941 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
     3001 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
     3061 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     3121 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     3181 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     3241 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     3301 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     3361 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     3421 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     3481 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     3541 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     3601 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     3661 aagttttttt cttccatttc aggtgtcgtg attcacctgc agcccaagct taccgccacc
     3721 atggcccagt ccaagcacgg cctgaccaag gagatgacca tgaagtaccg catggagggc
     3781 tgcgtggacg gccacaagtt cgtgatcacc ggcgagggca tcggctaccc cttcaagggc
     3841 aagcaggcca tcaacctgtg cgtggtggag ggcggcccct tgcccttcgc cgaggacatc
     3901 ttgtccgccg ccttcatgta cggcaaccgc gtgttcaccg agtaccccca ggacatcgtc
     3961 gactacttca agaactcctg ccccgccggc tacacctggg accgctcctt cctgttcgag
     4021 gacggcgccg tgtgcatctg caacgccgac atcaccgtga gcgtggagga gaactgcatg
     4081 taccacgagt ccaagttcta cggcgtgaac ttccccgccg acggccccgt gatgaagaag
     4141 atgaccgaca actgggagcc ctcctgcgag aagatcatcc ccgtgcccaa gcagggcatc
     4201 ttgaagggcg acgtgagcat gtacctgctg ctgaaggacg gtggccgctt gcgctgccag
     4261 ttcgacaccg tgtacaaggc caagtccgtg ccccgcaaga tgcccgactg gcacttcatc
     4321 cagcacaagc tgacccgcga ggaccgcagc gacgccaaga accagaagtg gcacctgacc
     4381 gagcacgcca tcgcctccgg ctccgccttg cccgaatttg gcagcggcca gtgcaccaac
     4441 tatgccctgc tgaaactggc tggcgatgtg gaaagcaacc ccggacccat gaccgagtac
     4501 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     4561 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     4621 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     4681 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     4741 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     4801 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     4861 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     4921 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     4981 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     5041 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac
     5101 gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcggcgtc tcgagtctag
     5161 agggcccgac gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact
     5221 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatggctttg
     5281 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     5341 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     5401 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     5461 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     5521 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     5581 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     5641 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     5701 ctgcggcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     5761 tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta
     5821 gcaatacagc agctatcaat gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     5881 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     5941 ttagccactt tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac
     6001 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact
     6061 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
     6121 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
     6181 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
     6241 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
     6301 gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
     6361 gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt
     6421 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6481 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6541 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     6601 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     6661 tcagagagtg agaggccttg acattgctag cgtttaccgt cgacctctag ctagagcttg
     6721 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     6781 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     6841 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     6901 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     6961 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7021 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7081 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7141 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7201 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7261 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7321 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7381 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7441 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7501 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7561 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7621 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7681 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7741 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     7801 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     7861 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     7921 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     7981 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8041 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8101 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8161 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8221 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8281 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8341 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8401 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8461 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8521 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8581 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     8641 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     8701 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     8761 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     8821 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     8881 cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata
     8941 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     9001 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
     9061 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
     9121 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
     9181 ttaaatatgt actacaaact tagtagtttt taaagaaatt gtatttgtta aatatgtact
     9241 acaaacttag tagt
//
