LOCUS       V001637                14900 bp    DNA     circular SYN 21-DEC-2021
DEFINITION  Exported.
ACCESSION   V001637
VERSION     V001637
KEYWORDS    pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14900)
  AUTHORS   Thakore PI, D'Ippolito AM, Song L, Safi A, Shivakumar NK, Kabadi AM,
            Reddy TE, Crawford GE, Gersbach CA
  TITLE     Highly specific epigenome editing by CRISPR-Cas9 repressors for
            silencing of distal regulatory elements.
  JOURNAL   Nat Methods. 2015 Dec;12(12):1143-9. doi: 10.1038/nmeth.3630. Epub
            2015 Oct 26.
   PUBMED   26501517
REFERENCE   2  (bases 1 to 14900)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 14900)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; doi:
            "10.1038/nmeth.3630"; journalName: "Nat Methods"; date: "2015-12";
            volume: "12"; issue: "12"; pages: "1143-9"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2..21)
                     /label="SV40pro-F"
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     248..268
                     /label="pBABE 3'"
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(277..705)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    complement(751..975)
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     LTR             complement(1007..1187)
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     1692..1708
                     /label="pBluescriptKS"
                     /note="For pBluescript vector"
     primer_bind     1693..1709
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(1712..2300)
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     protein_bind    2325..2358
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(2415..3011)
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     CDS             complement(3012..3065)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(3072..3092)
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             complement(3159..3353)
                     /label="KRAB"
                     /note="Kruppel-associated box (KRAB) transcriptional
                     repression domain from the human zinc finger protein ZNF10
                     (Margolin et al., 1994)"
     CDS             complement(3387..3407)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             complement(3420..7523)
                     /label="dCas9"
                     /note="catalytically dead mutant of the Cas9 endonuclease
                     from the Streptococcus pyogenes Type II CRISPR/Cas system"
     CDS             complement(7533..7553)
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             complement(7560..7583)
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     regulatory      7625..7634
                     /label="Kozak sequence"
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7663..8874)
                     /label="UbC promoter"
                     /note="human ubiquitin C promoter"
     promoter        8907..9147
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        9172..9247
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     protein_bind    complement(9258..9291)
                     /label="loxP"
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
     misc_feature    complement(9335..9452)
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     CDS             complement(9560..9601)
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     CDS             complement(9750..9794)
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     misc_feature    complement(9979..10212)
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     misc_feature    complement(10705..10830)
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     LTR             complement(10877..11057)
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(11072..11274)
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(11275..11654)
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     11826..11845
                     /label="pRS-marker"
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     promoter        11920..12024
                     /label="AmpR promoter"
     CDS             12025..12882
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      13056..13644
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     13798..13815
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     protein_bind    13932..13953
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        13968..13998
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    14006..14022
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14030..14046
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     polyA_signal    complement(14083..14216)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(14349..14720)
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     promoter        complement(14739..14786)
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
ORIGIN
        1 gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa
       61 ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa
      121 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg
      181 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
      241 tttccacacc ctaactgaca cacattccac agaattaatt cgcgttaaat ttttgttaaa
      301 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
      361 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
      421 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
      481 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
      541 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
      601 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
      661 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtgggga taccccctag
      721 agccccagct ggttctttcc gcctcagaag ccatagagcc caccgcatcc ccagcatgcc
      781 tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc caccccaccc cccagaatag
      841 aatgacacct actcagacaa tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg
      901 gagtggcacc ttccagggtc aaggaaggca cgggggaggg gcaaacaaca gatggctggc
      961 aactagaagg cacagtcgag gctgatcagc gggtttaaac gggccctgct agagattttc
     1021 cacactgact aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca
     1081 cacactactt gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt
     1141 tagccagaga gctcccaggc tcagatctgg tctaaccaga gagacccagt acagtccgga
     1201 tgcagctctc gggccatgtg atgaaatgct aggcggctgt caaacctcca ctctaatact
     1261 tctctctccg ggtcatccat cccatgcagg ctcacagggt gtaacaagcg ggtgttctct
     1321 ccttcattgg cttcttctac cttctcttgc tcaactggta ctagcttgta gcaccatcca
     1381 aaggtcagtg gatatctgat ccctggccct ggtgtgtagt tctgccaatc agggaagtag
     1441 ccttgtgtgt ggtagatcca cagatcaagg atatcttgtc ttcgttggga gtgaattagc
     1501 ccttccagtc cccccttttc ttttaaaaag tggctaagat ctacagctgc cttgtaagtc
     1561 attggtctta aaggtacctg aggtgtgact ggaaaaccca cctcctcctc ctcttgtgct
     1621 tctagccagg cacaatcagc attggtagct gctgtattgc tacttgtgat tgctccatgt
     1681 ttttctaggt ctcgaggtcg acggtatcga tgcggggagg cggcccaaag ggagatccga
     1741 ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag caggccgcgg
     1801 gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt cccgcgcaga
     1861 atccaggtgg caacacaggc gagcagccaa ggaaaggacg atgatttccc cgacaacacc
     1921 acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag gcaggcggcg
     1981 atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag gagctgacag
     2041 gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca caccacgcca
     2101 cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga tttatacaag
     2161 gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat taaagcagcg
     2221 tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct ttcacaaatt
     2281 ttgtaatcca gaggttgatt atcgataagc ttgatatcga attcataact tcgtataatg
     2341 tatgctatac gaagttatct attatcaacc ggtcgtgctg cagaagctag taacggccgc
     2401 cagtgtgctg gtcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc
     2461 acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag gttgcggggc
     2521 gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac tccggggagc
     2581 acgacggcgc tgcccagacc cttgccctgg tggtcgggcg acacgccgac ggtggccagg
     2641 aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg
     2701 gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa caccgccccc
     2761 gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac
     2821 accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc
     2881 tcgatgtggc ggtccggatc gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg
     2941 gcgagggtgc gtacggccct ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg
     3001 tactcggtca tagggccggg attctcctcc acgtcaccgc atgttagaag acttcctctg
     3061 ccctcgctag caactttgcg tttctttttc ggaactgatg atttgatttc aaatgcagtc
     3121 tctgaatcag gatgggtctc ttggtgaatt tctctctcca ccagccaggg ctcttctccc
     3181 ttctccaacc ggaggatcac atctggctta gtaagctgat aacccaagga aaccaggttc
     3241 ttatagttct ccagcatcac atttctgtac aggatctgct gagcagtgtc cagcagcttc
     3301 cactcctccc tggtgaagtc cacaaacaca tccttgaagg tcaccagtgt ccgggaccag
     3361 gcagtcagtg acttagcatc gctagccacc ttcctcttct tcttggggtc agccctgctg
     3421 tctccaccga gctgagagag gtcgattctt gtttcataga gccccgtaat tgactgatga
     3481 atcagtgtgg cgtccaggac ctcctttgta gaggtgtacc gctttctgtc tatggtggtg
     3541 tcgaagtact tgaaggctgc aggcgcgccc aagttggtca gagtaaacaa gtggataatg
     3601 ttttctgcct gctccctgat gggcttatcc ctgtgcttat tgtaagcaga aagcacctta
     3661 tcgaggttag cgtcggcgag gatcactctt ttggagaatt cgcttatttg ctcgatgatc
     3721 tcatcaaggt agtgtttgtg ttgttccacg aacagctgct tctgctcatt atcttcggga
     3781 gaccctttga gcttttcata gtggctggcc agatacaaga aattaacgta tttagagggc
     3841 agtgccagct cgttaccttt ctgcagctcg cccgcactag cgagcattcg tttccggccg
     3901 ttttcaagct caaagagaga gtacttggga agcttaatga tgaggtcttt tttgacctct
     3961 ttatatcctt tcgcctcgag aaagtcgatg gggttttttt cgaagcttga tcgctccatg
     4021 attgtgatgc ccagcagttc cttgacgctt ttgagttttt tagacttccc tttctccact
     4081 ttggccacaa ccagtacact gtaagcgact gtaggagaat cgaatccgcc gtatttcttg
     4141 gggtcccaat cttttttgcg tgcgatcagc ttgtcgctgt tccttttcgg gaggatactt
     4201 tccttggaga agcctccggt ctgtacttcg gtctttttaa cgatgttcac ctgcggcatg
     4261 gacaggacct tccggactgt cgcgaaatcc ctacccttgt cccacacgat ttctcctgtt
     4321 tctccgtttg tttcgataag tggtcgcttc cgaatctctc cattggccag tgtaatctcg
     4381 gtcttgaaaa aattcataat attgctgtaa aagaagtact tagcggtggc cttgcctatt
     4441 tcctgctcag actttgcgat cattttccta acatcgtaca ctttatagtc tccgtaaaca
     4501 aattcagatt caagcttggg atattttttg ataagtgcag tgcctaccac tgcattcagg
     4561 taggcatcat gcgcatggtg gtaattgttg atctctctca ccttataaaa ctgaaagtcc
     4621 tttctgaaat ctgagaccag cttagacttc agagtaataa ctttcacctc tcgaatcagt
     4681 ttgtcatttt catcgtactt ggtgttcatg cgtgaatcga gaatttgggc cacgtgcttg
     4741 gtgatctggc gtgtctcaac aagctgcctt ttgatgaagc cggctttatc caactcagac
     4801 aggccacctc gttcagcctt agtcagatta tcgaacttcc gttgtgtgat cagtttggcg
     4861 ttcagcagct gccgccaata atttttcatt ttcttgacaa cttcttctga ggggacgtta
     4921 tcactcttcc ctctattttt atcggatctt gtcaacactt tattatcaat agaatcatct
     4981 ttgagaaaag actggggcac gatggcatcc acgtcgtagt cggagagccg attgatgtcc
     5041 agttcctgat ccacgtacat gtccctgccg ttctgcaggt agtacaggta gagcttctca
     5101 ttctgaagct gggtgttttc aactgggtgt tccttaagga tttgggaccc cagttctttt
     5161 ataccctctt caatcctctt catcctttcc ctactgttct tctgtccctt ctgggtagtt
     5221 tggttctctc gggccatctc gataacgata ttctcgggct tatgccttcc cattactttg
     5281 acgagttcat ccacgacctt aacggtctgc agtattccct ttttgatagc tgggctacct
     5341 gcaagattag cgatgtgctc gtgaagactg tccccctggc cagaaacttg tgctttctgg
     5401 atgtcctcct taaaggtgag agagtcatca tggatcaact gcatgaagtt ccggttggca
     5461 aatccatcgg acttaagaaa atccaggatt gtctttccac tctgcttgtc tcggatccca
     5521 ttgatcagtt ttcttgacag ccgcccccat cctgtatatc ggcgcctctt gagctgtttc
     5581 atgactttgt cgtcgaagag atgagcgtaa gttttcaagc gttcttcaat catctcccta
     5641 tcttcaaaca acgtaagggt gaggacaatg tcctcaagaa tgtcctcgtt ctcctcattg
     5701 tccaggaagt ccttgtcttt aatgattttc aggagatcgt gatacgttcc cagggatgcg
     5761 ttgaagcgat cctccactcc gctgatttca acagagtcga aacattcaat ctttttgaaa
     5821 tagtcttctt tgagctgttt cacggtaact ttccggttcg tcttgaagag gaggtccacg
     5881 atagctttct tctgctctcc agacaggaat gctggctttc tcatcccttc tgtgacgtat
     5941 ttgaccttgg tgagctcgtt ataaactgtg aagtactcgt acagcagaga gtgtttagga
     6001 agcacctttt cgttaggcag atttttatca aagttagtca tcctttcgat gaaggactgg
     6061 gcagaggccc ccttatccac gacttcctcg aagttccagg gagtgatggt ctcttctgat
     6121 ttgcgagtca tccacgcgaa tctggaattt ccccgggcga gggggcctac atagtagggt
     6181 atccgaaatg tgaggatttt ctcaatcttt tccctgttat ctttcaaaaa ggggtagaaa
     6241 tcctcttgcc gcctgaggat agcgtgcagt tcgcccaggt gaatctggtg ggggatgctt
     6301 ccattgtcga aagtgcgctg tttgcgcaac agatcttctc tgttaagctt taccagcagc
     6361 tcctcggtgc cgtccatttt ttccaagatg ggcttaataa atttgtaaaa ttcctcctgg
     6421 cttgctccgc cgtcaatgta tccggcgtag ccatttttag actgatcgaa gaaaatttcc
     6481 ttgtacttct caggcagttg ctgtctgaca agggccttca gcaaagtcaa gtcttggtgg
     6541 tgctcatcat agcgcttgat catactagcg ctcagcggag ctttggtgat ctccgtgttc
     6601 actcgcagaa tatcactcag cagaatggcg tctgacaggt tctttgccgc caaaaaaagg
     6661 tctgcgtact ggtcgccgat ctgggccagc agattgtcga gatcatcatc gtaggtgtct
     6721 ttgctcagtt gaagcttggc atcttcggcc aggtcgaagt tagatttaaa gttgggggtc
     6781 agcccgagtg acagggcgat aagattacca aacaggccgt tcttcttctc cccagggagc
     6841 tgtgcgatga ggttttcgag ccgccgggat ttggacagcc tagcgctcag gattgctttg
     6901 gcgtcaactc cggatgcgtt gatcgggttc tcttcgaaaa gctgattgta agtctgaacc
     6961 agttggataa agagtttgtc gacatcgctg ttgtctgggt tcaggtcccc ctcgatgagg
     7021 aagtgtcccc gaaatttgat catatgcgcc agcgcgagat agatcaaccg caagtcagcc
     7081 ttatcagtac tgtctacaag cttcttcctc agatgatata tggttgggta cttttcatgg
     7141 tacgccacct cgtccacgat attgccaaag attgggtggc gctcgtgctt tttatcctcc
     7201 tccaccaaaa aggactcctc cagcctatgg aagaaagagt catccacctt agccatctca
     7261 ttactaaaga tctcctgcag gtagcagatc cgattctttc tgcgggtata tctgcgccgt
     7321 gctgttcttt tgagccgcgt ggcttcggcg gtttccccgg agtcgaacag gagggcgcca
     7381 atgaggttct tctttatgct gtggcgatcg gtattgccca gaactttgaa ttttttgctc
     7441 ggcaccttgt actcgtccgt aatgacggcc cagccgacgc tgtttgtgcc gatggcgagc
     7501 ccaatggagt acttcttgtc cattccgcgg cccaccttcc tcttcttctt gggggccatc
     7561 ttgtcatcgt catccttgta atcgatgtca tgatctttat aatcaccgtc atggtctttg
     7621 tagtccatgg tggctctaga gtcgacctgc agcccaagct tcgtctaaca aaaaagccaa
     7681 aaacggccag aatttagcgg acaatttact agtctaacac tgaaaattac atattgaccc
     7741 aaatgattac atttcaaaag gtgcctaaaa aacttcacaa aacacactcg ccaaccccga
     7801 gcgcatagtt caaaaccgga gcttcagcta cttaagaaga taggtacata aaaccgacca
     7861 aagaaactga cgcctcactt atccctcccc tcaccagagg tccggcgcct gtcgattcag
     7921 gagagcctac cctaggcccg aaccctgcgt cctgcgacgg agaaaagcct accgcacacc
     7981 taccggcagg tggccccacc ctgcattata agccaacaga acgggtgacg tcacgacacg
     8041 acgagggcgc gcgctcccaa aggtacgggt gcactgccca acggcaccgc cataactgcc
     8101 gcccccgcaa cagacgacaa accgagttct ccagtcagtg acaaacttca cgtcagggtc
     8161 cccagatggt gccccagccc atctcacccg aataagagct ttcccgcatt agcgaaggcc
     8221 tcaagacctt gggttcttgc cgcccaccat gccccccacc ttgtttcaac gacctcacag
     8281 cccgcctcac aagcgtcttc cattcaagac tcgggaacag ccgccatttt gctgcgctcc
     8341 ccccaacccc cagttcaggg caaccttgct cgcggaccca gactacagcc cttggcggtc
     8401 tctccacacg cttccgtccc accgagcggc ccggcggcca cgaaagcccc ggccagccca
     8461 gcagcccgct actcaccaag tgacgatcac agcgatccac aaacaagaac cgcgacccaa
     8521 atcccggctg cgacggaact agctgtgcca cacccggcgc gtccttatat aatcatcggc
     8581 gttcaccgcc ccacggagat ccctccgcag aatcgccgag aagggactac ttttcctcgc
     8641 ctgttccgct ctctggaaag aaaaccagtg ccctagagtc acccaagtcc cgtcctaaaa
     8701 tgtccttctg ctgatactgg ggttctaagg ccgagtctta tgagcagcgg gccgctgtcc
     8761 tgagcgtccg ggcggaagga tcaggacgct cgctgcgccc ttcgtctgac gtggcagcgc
     8821 tcgccgtgag gaggggggcg cccgcgggag gcgccaaaac ccggcgcgga ggccagatct
     8881 ggagccgaca cgggttaatt aattaagagg gcctatttcc catgattcct tcatatttgc
     8941 atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga
     9001 tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa
     9061 aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct
     9121 tggctttata tatcttgtgg aaaggacgaa acaccggaga cggacgtctc agttttagag
     9181 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
     9241 tcggtgcttt ttttttaata acttcgtata atgtatgcta tacgaagtta ttaattaacc
     9301 aaactggatc tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt
     9361 tttgtaattc tttagtttgt atgtctgttg ctattatgtc tactattctt tcccctgcac
     9421 tgtacccccc aatcccccct tttcttttaa aattgtggat gaatactgcc atttgtctgc
     9481 agaattggcg cacgcagtgc cgatccgttc actaatcgaa tggatctgtc tctgtctctc
     9541 tctccacctt cttcttctat tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg
     9601 gtctgaaacg ataatggtga atatccctgc ctaactctat tcactataga aagtacagca
     9661 aaaactattc ttaaacctac caagcctcct actatcatta tgaataattt tatataccac
     9721 agccaatttg ttatgttaaa ccaattccac aaacttgccc atttatctaa ttccaataat
     9781 tcttgttcat tcttttcttg ctggttttgc gattcttcaa ttaaggagtg tattaagctt
     9841 gtgtaattgt taatttctct gtcccactcc atccaggtcg tgtgattcca aatctgttcc
     9901 agagatttat tactccaact agcattccaa ggcacagcag tggtgcaaat gagttttcca
     9961 gagcaacccc aaatccccag gagctgttga tcctttaggt atctttccac agccaggatt
    10021 cttgcctgga gctgcttgat gccccagact gtgagttgca acagatgctg ttgcgcctca
    10081 atagccctca gcaaattgtt ctgctgctgc actataccag acaataattg tctggcctgt
    10141 accgtcagcg tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg
    10201 aacaaagctc ctattcccac tgctcttttt tctctctgca ccactcttct ctttgccttg
    10261 gtgggtgcta ctcctaatgg ttcaattttt actactttat atttatataa ttcacttctc
    10321 caattgtccc tcatatctcc tcctccaggt ctgaagatca gcggccgctt gctgtgcggt
    10381 ggtcttactt ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat
    10441 ctctatcctt tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc
    10501 ttctgatcct gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga
    10561 tgtttctaac aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat
    10621 atgttttaat ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga
    10681 tctaattctc ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc
    10741 gctagtcaaa atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc
    10801 ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc
    10861 cctgttcggg cgccactgct agagattttc cacactgact aaaagggtct gagggatctc
    10921 tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt
    10981 tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg
    11041 tctaaccaga gagacccagt acaggcaaaa cgcgctgctt atatagacct cccaccgtac
    11101 acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg
    11161 ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc
    11221 ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt
    11281 aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta
    11341 ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg
    11401 catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg
    11461 acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa
    11521 tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg
    11581 gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt aataactagt
    11641 caataatcaa tgtcaacgcg tatatctggc ccgtacatcg cgaagcagcg caaaacgcct
    11701 aaccctaagc agattcttca tgcaattgtc ggtcaagcct tgccttgttg tagcttaaat
    11761 tttgctcgcg cactactcag cgacctccaa cacacaagca gggagcagat actggcttaa
    11821 ctatgcggca tcagagcaga ttgtactgag agtgcaccat aggggatcgg gagatctccc
    11881 gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
    11941 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
    12001 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
    12061 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
    12121 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
    12181 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
    12241 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
    12301 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
    12361 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
    12421 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
    12481 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
    12541 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
    12601 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
    12661 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
    12721 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
    12781 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
    12841 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
    12901 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
    12961 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
    13021 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
    13081 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
    13141 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
    13201 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
    13261 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
    13321 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
    13381 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    13441 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    13501 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    13561 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    13621 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
    13681 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
    13741 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
    13801 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
    13861 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
    13921 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
    13981 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
    14041 tatgaccatg attacgccaa gctctagcta gaggtcgacg gtatacagac atgataagat
    14101 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
    14161 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttgggg
    14221 tgggcgaaga actccagcat gagatccccg cgctggagga tcatccagcc ggcgtcccgg
    14281 aaaacgattc cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa atctcgtagc
    14341 acgtgtcagt cctgctcctc ggccacgaag tgcacgcagt tgccggccgg gtcgcgcagg
    14401 gcgaactccc gcccccacgg ctgctcgccg atctcggtca tggccggccc ggaggcgtcc
    14461 cggaagttcg tggacacgac ctccgaccac tcggcgtaca gctcgtccag gccgcgcacc
    14521 cacacccagg ccagggtgtt gtccggcacc acctggtcct ggaccgcgct gatgaacagg
    14581 gtcacgtcgt cccggaccac accggcgaag tcgtcctcca cgaagtcccg ggagaacccg
    14641 agccggtcgg tccagaactc gaccgctccg gcgacgtcgc gcgcggtgag caccggaacg
    14701 gcactggtca acttggccat ggtttagttc ctcaccttgt cgtattatac tatgccgata
    14761 tactatgccg atgattaatt gtcaacacgt gctgatcaga tccgaaaatg gatatacaag
    14821 ctcccgggag ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga
    14881 atagctcaga ggcagaggcg
//
