LOCUS       V012712                 8763 bp    DNA     circular SYN 08-APR-2021
DEFINITION  Exported.
ACCESSION   V012712
VERSION     V012712
KEYWORDS    pLVX-ZsGreen1-C1.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8763)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2201..2504
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2807..3499
                     /label="ZsGreen1"
                     /note="Zoanthus green fluorescent protein"
     misc_feature    3500..3565
                     /label="MCS"
                     /note="multiple cloning site"
     promoter        3598..4097
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             4118..4714
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    4731..5319
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5526..6159
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6288..6304)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6312..6328)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6336..6366)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6381..6402)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6690..7278)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7452..8309)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8310..8414)
                     /label="AmpR promoter"
     polyA_signal    8462..8596
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctaccggtc gccaccatgg cccagtccaa
     2821 gcacggcctg accaaggaga tgaccatgaa gtaccgcatg gagggctgcg tggacggcca
     2881 caagttcgtg atcaccggcg agggcatcgg ctaccccttc aagggcaagc aggccatcaa
     2941 cctgtgcgtg gtggagggcg gccccttgcc cttcgccgag gacatcttgt ccgccgcctt
     3001 catgtacggc aaccgcgtgt tcaccgagta cccccaggac atcgtcgact acttcaagaa
     3061 ctcctgcccc gccggctaca cctgggaccg ctccttcctg ttcgaggacg gcgccgtgtg
     3121 catctgcaac gccgacatca ccgtgagcgt ggaggagaac tgcatgtacc acgagtccaa
     3181 gttctacggc gtgaacttcc ccgccgacgg ccccgtgatg aagaagatga ccgacaactg
     3241 ggagccctcc tgcgagaaga tcatccccgt gcccaagcag ggcatcttga agggcgacgt
     3301 gagcatgtac ctgctgctga aggacggtgg ccgcttgcgc tgccagttcg acaccgtgta
     3361 caaggccaag tccgtgcccc gcaagatgcc cgactggcac ttcatccagc acaagctgac
     3421 ccgcgaggac cgcagcgacg ccaagaacca gaagtggcac ctgaccgagc acgccatcgc
     3481 ctccggctcc gccttgccct ccggactcag atctcgagct caagcttcga attctgcagt
     3541 cgacggtacc gcgggcccgg gatccaccgg atctagataa ctgatcataa ttctaccggg
     3601 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     3661 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     3721 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     3781 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     3841 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     3901 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     3961 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     4021 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     4081 catctccggg cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt
     4141 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     4201 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     4261 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     4321 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     4381 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     4441 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     4501 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     4561 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     4621 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     4681 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaccg cgtctggaac aatcaacctc
     4741 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     4801 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     4861 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     4921 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     4981 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     5041 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     5101 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     5161 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     5221 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     5281 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg
     5341 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     5401 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     5461 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag
     5521 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     5581 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     5641 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     5701 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     5761 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     5821 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     5881 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     5941 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     6001 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6061 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6121 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     6181 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt
     6241 gctagcgttt taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     6301 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     6361 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6421 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     6481 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6541 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6601 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6661 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6721 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6781 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6841 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     6901 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6961 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7021 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7081 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7141 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7201 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7261 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7321 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7381 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7441 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7501 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7561 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7621 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7681 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7741 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7801 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7861 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7921 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7981 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8041 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     8101 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8161 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8221 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8281 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8341 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8401 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     8461 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     8521 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     8581 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
     8641 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
     8701 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
     8761 agt
//
