LOCUS       V013082                 8940 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013082
VERSION     V013082
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2201..2504
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             3673..3696
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     promoter        3728..4227
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             4248..4844
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    4861..5449
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5656..6289
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6418..6434)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6442..6458)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6466..6496)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6511..6532)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6820..7408)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7582..8439)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8440..8544)
                     /label="AmpR promoter"
     polyA_signal    8592..8726
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 gccaccatgg agatctcatc aggcttgctg ttcctgggcc acctaatagt gctcacctat
     2881 ggccacccca ccctaaaaac acctgagagt gtgacaggga cctggaaagg agatgtgaag
     2941 attcagtgca tctatgatcc cctgagaggc tacaggcaag ttttggtgaa atggctggta
     3001 agacacggct ctgactccgt caccatcttc ctacgtgact ccactggaga ccatatccag
     3061 caggcaaagt acagaggccg cctgaaagtg agccacaaag ttccaggaga tgtgtccctc
     3121 caaataaata ccctgcagat ggatgacagg aatcactata catgtgaggt cacctggcag
     3181 actcctgatg gaaaccaagt aataagagat aagatcattg agctccgtgt tcggaaatat
     3241 aatccaccta gaatcaatac tgaagcacct acaaccctgc actcctcttt ggaagcaaca
     3301 actataatga gttcaacctc tgacttgacc actaatggga ctggaaaact tgaggagacc
     3361 attgctggtt cagggaggaa cctgccaatc tttgccataa tcttcatcat ctccctttgc
     3421 tgcatagtag ctgtcaccat accttatatc ttgttccgct gcaggacatt ccaacaagag
     3481 tatgtctatg gagtgagcag ggtgtttgcc aggaagacaa gcaactctga agaaaccaca
     3541 agggtgacta ccatcgcaac tgatgaacca gattcccagg ctctgattag tgactactct
     3601 gatgatcctt gcctcagcca ggagtaccaa ataaccatca gatcaacaat gtctattcct
     3661 gcctgcgaat tcgattacaa ggatgacgac gataagtgag gatcccgcga ctctagataa
     3721 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     3781 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     3841 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     3901 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     3961 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     4021 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     4081 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     4141 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     4201 tcctcttcct catctccggg cctttcgacc tgcagcccaa gcttaccatg accgagtaca
     4261 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     4321 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     4381 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     4441 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     4501 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     4561 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     4621 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     4681 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     4741 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     4801 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaccg cgtctggaac
     4861 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     4921 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     4981 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     5041 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     5101 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     5161 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     5221 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     5281 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     5341 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     5401 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat
     5461 tctgcagtcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     5521 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     5581 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     5641 aagaaaagag gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     5701 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
     5761 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
     5821 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
     5881 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
     5941 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
     6001 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
     6061 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
     6121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     6181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     6241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     6301 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     6361 ccttgacatt gctagcgttt taccgtcgac ctctagctag agcttggcgt aatcatggtc
     6421 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     6481 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     6541 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     6601 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     6661 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6721 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6781 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6841 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6901 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6961 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7021 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7081 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7141 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7201 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7261 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7321 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7381 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7441 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7501 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     7561 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     7621 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7681 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7741 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     7801 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     7861 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     7921 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     7981 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8041 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8101 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8161 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     8221 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     8281 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     8341 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     8401 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     8461 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     8521 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     8581 atcgggagat caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     8641 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     8701 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
     8761 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
     8821 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
     8881 caaacttagt agtttttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
