LOCUS       V013057                 8791 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013057
VERSION     V013057
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8791)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2201..2504
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2827..3519
                     /label="TurboRFP"
                     /note="red fluorescent protein from Entacmaea quadricolor"
     promoter        3579..4078
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             4099..4695
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    4712..5300
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5507..6140
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6269..6285)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6293..6309)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6317..6347)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6362..6383)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6671..7259)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7433..8290)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8291..8395)
                     /label="AmpR promoter"
     polyA_signal    8443..8577
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 gccaccatga gcgagctgat caaggagaac atgcacatga agctgtacat ggagggcacc
     2881 gtgaacaacc accacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc
     2941 cagaccatga agatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg
     3001 gctaccagct tcatgtacgg cagcaaagcc ttcatcaacc acacccaggg catccccgac
     3061 ttctttaagc agtccttccc tgagggcttc acatgggaga gaatcaccac atacgaagac
     3121 gggggcgtgc tgaccgctac ccaggacacc agcttccaga acggctgcat catctacaac
     3181 gtcaagatca acggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacacgc
     3241 ggctgggagg ccaacaccga gatgctgtac cccgctgacg gcggcctgag aggccacagc
     3301 cagatggccc tgaagctcgt gggcgggggc tacctgcact gctccttcaa gaccacatac
     3361 agatccaaga aacccgctaa gaacctcaag atgcccggct tccacttcgt ggaccacaga
     3421 ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg agcagcacga gatggctgtg
     3481 gccaagtact gcgacctccc tagcaaactg gggcacagag aattctgcag tcgacggtac
     3541 cgcgggcccg ggatcccgcg actctagata attctaccgg gtaggggagg cgcttttccc
     3601 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
     3661 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
     3721 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
     3781 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
     3841 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
     3901 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
     3961 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
     4021 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac
     4081 ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     4141 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     4201 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     4261 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     4321 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     4381 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     4441 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     4501 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     4561 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     4621 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     4681 cgcaagcccg gtgcctgacc gcgtctggaa caatcaacct ctggattaca aaatttgtga
     4741 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     4801 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     4861 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     4921 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     4981 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     5041 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     5101 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     5161 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     5221 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     5281 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga
     5341 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     5401 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     5461 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt
     5521 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     5581 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     5641 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     5701 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     5761 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     5821 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     5881 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     5941 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     6001 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6061 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6121 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     6181 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt ttaccgtcga
     6241 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     6301 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     6361 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6421 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6481 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6541 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6601 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6661 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     6721 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6781 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6841 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     6901 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     6961 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7021 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7081 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7141 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7201 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7261 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7321 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7381 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7441 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7501 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7561 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7621 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7681 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     7741 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     7801 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     7861 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     7921 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     7981 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8041 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8101 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8161 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8221 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8281 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8341 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8401 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct
     8461 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     8521 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
     8581 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
     8641 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
     8701 taaagaaatt gtatttgtta aatatgtact acaaacttag tagtttttaa agaaattgta
     8761 tttgttaaat atgtactaca aacttagtag t
//
