LOCUS V023471 12054 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V023471 VERSION V023471 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers polyA_signal 216..350 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" promoter 398..502 /gene="bla" /label="AmpR promoter" CDS 503..1363 /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" rep_origin 1534..2119 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" protein_bind 2407..2428 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" promoter 2443..2473 /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 2481..2497 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" primer_bind 2505..2521 /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" LTR 2650..3283 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 3491..4079 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" CDS complement(4093..4692) /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" promoter complement(4701..5030) /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS complement(5041..5787) /codon_start=1 /product="modified rtTA protein that binds tightly to promoters containing the tet operator in the presence of doxycycline" /transl_table=1 /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA DALDDFDLDMLPADALDDFDLDMLPG*" /label="Tet-On® 3G" promoter complement(5806..6316) /note="human phosphoglycerate kinase 1 promoter" /label="hPGK promoter" promoter 6333..6700 /note="3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On® 3G, modified to eliminate binding sites for endogenous mammalian transcription factors" /label="TRE3GS promoter" CDS 6713..7429 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK" /label="EGFP" CDS 7478..9550 /label="TBC1D15(NM_022771)" /note="TBC1D15(NM_022771)" /gene="TBC1D15" polyA_signal 9734..9868 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" misc_feature 9912..10027 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" CDS complement(10290..10334) /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 10519..10752 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" misc_feature 11249..11374 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" LTR 11421..12054 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" ORIGIN 1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaaact actaagtttg 61 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg 121 tttagagtaa atgaaaccac taggtaattg gcagtggtaa tagggtatgg ggtgggaagt 181 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg 241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga 361 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 1561 ctgctgcttg caaacaaaaa aaccgctacc agcggtggtt tgtttgccgg atcaagagct 1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 2161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 2221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 2281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 2341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 2401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 2461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 2521 ccatgattac gccaagctct agctagaggt cgacggtaaa acgctagcaa tgtcaaggcc 2581 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg 2641 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac 2701 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag 2761 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc 2821 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac 2881 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag 2941 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga 3001 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca 3061 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta 3121 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct 3181 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga 3241 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct cttttctttt 3301 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt 3361 gtgactggaa aaacccacct cctcctcctc ttgtgcttct agccaggcac aatcagcatt 3421 ggtagctgct gtattgctac ttgtgattgc tccatgtttt tctaggtctc gactgcagaa 3481 ttaattccag gcggggaggc ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga 3541 agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc cgctggattg 3601 agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg 3661 agcagccatg gaaaggacgt cagcttcccc gacaacacca cggaattgtc agtgcccaac 3721 agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc cgtggcaata 3781 gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat gccccaacca 3841 gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac aacgggccac 3901 aactcctcat aaagagacag caaccaggat ttatacaagg aggagaaaat gaaagccata 3961 cgggaagcaa tagcatgata caaaggcatt aaagcagcgt atccacatag cgtaaaagga 4021 gcaacatagt taagaatacc agtcaatctt tcacaaattt tgtaatccag aggttgattg 4081 ttccagacgc gttcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg 4141 cacctcgacg tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg 4201 cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag 4261 cacgacggcg ctgcccagac ccttgccctg gtggtcgggc gagacgccga cggtggccag 4321 gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc 4381 ggccagccgg gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc 4441 cgcttcgacg ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca 4501 caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg 4561 ctcgatgtgg cggtccgggt cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc 4621 ggcgagggtg cgtacggccc gggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt 4681 gtactcggtc atggtcgcgt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac 4741 ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt 4801 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt 4861 aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct 4921 ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata 4981 cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac attcgcgcgt 5041 ttacccgggg agcatgtcaa ggtcaaaatc gtcaagagcg tcagcaggca gcatatcaag 5101 gtcaaagtcg tcaagggcat cggctgggag catgtctaag tcaaaatcgt caagggcgtc 5161 ggtcggcccg ccgctttcgc actttagctg tttctccagg ccacatatga ttagttccag 5221 gccgaaaagg aaggcaggtt cggctccctg ccggtcgaac agctcaattg cttgtttcag 5281 aagtgggggc atagaatcgg tggtaggtgt ctctctttcc tcttttgcta cttgatgctc 5341 ctgttcctcc aatacgcagc ccagtgtaaa gtggcccacg gcggacagag cgtacagtgc 5401 gttctccagg gagaagcctt gctgacacag gaacgcgagc tgattttcca gggtttcgta 5461 ctgtttctct gttgggcggg tgccgagatg cactttagcc ccgtcgcgat gtgagaggag 5521 agcacagcgg tatgacttgg cgttgttccg cagaaagtct tgccatgact cgccttccag 5581 ggggcaggag tgggtatgat gcctgtccag catctcgatt ggcagggcat cgagcagggc 5641 ccgcttgttc ttcacgtgcc agtacagggt aggctgctca actcccagct tttgagcgag 5701 tttccttgtc gtcaggcctt cgataccgac tccattgagt aattccagag cagagtttat 5761 gactttgctc ttgtccagtc tagacatggt aattcgatga tccccctggg gagagaggtc 5821 ggtgattcgg tcaacgaggg agccgactgc cgacgtgcgc tccggaggct tgcagaatgc 5881 ggaacaccgc gcgggcagga acagggccca cactaccgcc ccacaccccg cctcccgcac 5941 cgccccttcc cggccgctgc tctcggcgcg ccctgctgag cagccgctat tggccacagc 6001 ccatcgcggt cggcgcgctg ccattgctcc ctggcgctgt ccgtctgcga gggtactagt 6061 gagacgtgcg gcttccgttt gtcacgtccg gcacgccgcg aaccgcaagg aaccttcccg 6121 acttaggggc ggagcaggaa gcgtcgccgg ggggcccaca agggtagcgg cgaagatccg 6181 ggtgacgctg cgaacggacg tgaagaatgt gcgagaccca gggtcggcgc cgctgcgttt 6241 cccggaacca cgcccagagc agccgcgtcc ctgcgcaaac ccagggctgc cttggaaaag 6301 gcgcaacccc aaccccgtgg aattatcacc tcgagtttac tccctatcag tgatagagaa 6361 cgtatgaaga gtttactccc tatcagtgat agagaacgta tgcagacttt actccctatc 6421 agtgatagag aacgtataag gagtttactc cctatcagtg atagagaacg tatgaccagt 6481 ttactcccta tcagtgatag agaacgtatc tacagtttac tccctatcag tgatagagaa 6541 cgtatatcca gtttactccc tatcagtgat agagaacgta taagctttgc ttatgtaaac 6601 cagggcgcct ataaaagagt gctgattttt tgagtaaact tcaattccac aacacttttg 6661 tcttatacca actttccgta ccacttccta ccctcgtaaa gaattcgcca ccatggtgag 6721 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt 6781 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct 6841 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac 6901 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga 6961 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga 7021 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg 7081 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga 7141 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa 7201 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta 7261 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag 7321 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga 7381 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt ccggactcag 7441 atctcgaggc ggcggaggga gtggaggagg gggaagcatg gcggcggcgg gtgttgtgag 7501 cgggaagatt atatatgaac aagaaggagt atatattcac tcatcttgtg gaaagaccaa 7561 tgaccaagac ggcttgattt caggaatatt acgtgtttta gaaaaggatg ccgaagtaat 7621 agtggactgg agaccattgg atgatgcatt agattcctct agtattctct atgctagaaa 7681 ggactccagt tcagttgtag aatggactca ggccccaaaa gaaagaggtc atcgaggatc 7741 agaacatctg aacagttacg aagcagaatg ggacatggtt aatacagttt catttaaaag 7801 gaaaccacat accaatggag atgctccaag tcatagaaat gggaaaagca aatggtcatt 7861 cctgttcagt ttgacagacc tgaaatcaat caagcaaaac aaagagggta tgggctggtc 7921 ctatttggta ttctgtctaa aggatgacgt cgttctccct gctctacact ttcatcaagg 7981 agatagcaaa ctactgattg aatctcttga aaaatatgtg gtattgtgtg aatctccaca 8041 ggataaaaga acacttcttg tgaattgtca gaataagagt ctttcacagt cttttgaaaa 8101 tcttcttgat gagccagcat atggtttaat acaagcagga ctgctagaca gaagaaagct 8161 gttgtgggcc attcaccact ggaaaaaaat taaaaaggac ccttatacgg caactatgat 8221 aggattttcc aaagtcacaa actacatttt tgacagtttg agaggcagcg atccctctac 8281 acatcaacga ccaccttcag aaatggcaga ttttcttagt gatgctattc caggtctaaa 8341 gataaatcaa caagaagaac caggatttga agtcatcaca agaattgatt tgggggaacg 8401 ccctgttgtt caaaggagag aaccggtatc actggaagaa tggactaaga acattgattc 8461 tgaaggaaga attttaaatg tagataatat gaagcagatg atatttagag ggggacttag 8521 tcatgcattg agaaagcaag catggaaatt tcttctgggt tattttccct gggacagtac 8581 caaggaggaa agaacccaat tacaaaagca aaaaactgat gaatacttca gaatgaaact 8641 gcagtggaaa tccatcagcc aggaacaaga gaaaagaaat tcgaggttaa gagattatag 8701 aagtcttatc gaaaaagatg ttaacagaac agatcgaaca aacaagtttt atgaaggcca 8761 agataatcca gggttgattt tacttcatga cattttgatg acctactgta tgtatgattt 8821 tgatttagga tatgttcaag gaatgagtga tttactttcc cctcttttat atgtgatgga 8881 aaatgaagtg gatgcctttt ggtgctttgc ctcttacatg gaccaaatgc atcagaattt 8941 tgaagaacaa atgcaaggca tgaagaccca gctaattcag ctgagtacct tacttcgatt 9001 gttagacagt ggattttgca gttacttaga atctcaggac tctggatacc tttatttttg 9061 cttcaggtgg cttttaatca gattcaaaag ggaatttagt tttctagata ttcttcgatt 9121 atgggaggta atgtggaccg aactaccatg tacaaatttc catcttcttc tctgttgtgc 9181 tattctggaa tcagaaaagc agcaaataat ggaaaagcat tatggcttca atgaaatact 9241 taagcatatc aatgaattgt ccatgaaaat tgatgtggaa gatatactct gcaaggcaga 9301 agcaatttct ctacagatgg taaaatgcaa ggaattgcca caagcagtct gtgagatcct 9361 tgggcttcaa ggcagtgaag ttacaacacc agattcagac gttggtgaag acgaaaatgt 9421 tgtcatgact ccttgtccta catctgcatt tcaaagtaat gccttgccta cactctctgc 9481 cagtggagcc agaaatgaca gcccaacaca gataccagtg tcctcagatg tctgcagatt 9541 aacacctgca tgaggatcca gaccacctcc cctgcgagct aagctggaca gccaatgacg 9601 ggtaagagag tgacattttt cactaaccta agacaggagg gccgtcagag ctactgccta 9661 atccaaagac gggtaaaagt gataaaaatg tatcactcca acctaagaca ggcgcagctt 9721 ccgagggatt tgagatccag acatgataag atacattgat gagtttggac aaaccaaaac 9781 tagaatgcag tgaaaaaaat gccttatttg tgaaatttgt gatgctattg ccttatttgt 9841 aaccattata agctgcaata aacaagttaa gtcgataaac tggatctctg ctgtccctgt 9901 aataaacccg aaaattttga atttttgtaa tttgtttttg tagttcttta gtttgtatgt 9961 ctgttgctat tatgtctact attctttccc ctgcactgta ccccccaatc cccccttttc 10021 ttttaaaggc gataccgtcg agatccgttc actaatcgaa tggatctgtc tctgtctctc 10081 tctccacctt cttcttctat tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg 10141 gtctgaaacg ataatggtga atatccctgc ctaactctat tcactataga aagtacagca 10201 aaaactattc ttaaacctac caagcctcct actatcatta tgaataattt tatataccac 10261 agccaatttg ttatgttaaa ccaattccac aaacttgccc atttatctaa ttccaataat 10321 tcttgttcat tcttttcttg ctggttttgc gattcttcaa ttaaggagtg tattaagctt 10381 gtgtaattgt taatttctct gtcccactcc atccaggtcg tgtgattcca aatctgttcc 10441 agagatttat tactccaact agcattccaa ggcacagcag tggtgcaaat gagttttcca 10501 gagcaacccc aaatccccag gagctgttga tcctttaggt atctttccac agccaggatt 10561 cttgcctgga gctgcttgat gccccagact gtgagttgca acagatgctg ttgcgcctca 10621 atagccctca gcaaattgtt ctgctgctgc actataccag acaataattg tctggcctgt 10681 accgtcagcg tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg 10741 aacaaagctc ctattcccac tgctcttttt tctctctgca ccactcttct ctttgccttg 10801 gtgggtgcta ctcctaatgg ttcaattttt actactttat atttatataa ttcacttctc 10861 caattgtccc tcatatctcc tcctccaggt ctgaagatca gcggccggcc gcttgctgtg 10921 cggtggtctt acttttgttt tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt 10981 ttatctctat cctttgatgc acacaataga gggttgctac tgtattatat aatgatctaa 11041 gttcttctga tcctgtctga agggatggtt gtagctgtcc cagtatttgt ctacagcctt 11101 ctgatgtttc taacaggcca ggattaactg cgaatcgttc tagctccctg cttgcccata 11161 ctatatgttt taatttatat tttttctttc cccctggcct taaccgaatt ttttcccatc 11221 gcgatctaat tctcccccgc ttaatactga cgctctcgca cccatctctc tccttctagc 11281 ctccgctagt caaaattttt ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc 11341 gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc tggtttccct ttcgctttca 11401 agtccctgtt cgggcgccac tgctagagat tttccacact gactaaaagg gtctgaggga 11461 tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa 11521 gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat 11581 ctggtctaac cagagagacc cagtacaggc aaaaagcagc tgcttatatg caggatctga 11641 gggctcgcca ctccccagtc ccgcccaggc cacgcctccc tggaaagtcc ccagcggaaa 11701 gtcccttgta gcaagctcga tatcagcagt tcttgaagta ctccggatgc agctctcggg 11761 ccacgtgatg aaatgctagg cggctgtcaa acctccactc taacacttct ctctccgggt 11821 catccatccc atgcaggctc acagggtgta acaagctggt gttctctcct ttattggcct 11881 cttctacctt atctggctca actggtacta gcttgtagca ccatccaaag gtcagtggat 11941 atctgacccc tggccctggt gtgtagttct gctaatcagg gaagtagcct tgtgtgtggt 12001 agatccacag atcaaggata tcttgtcttc tttgggagtg aattagccct tcca //