LOCUS V040221 11267 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V040221 VERSION V040221 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers polyA_signal 216..350 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" promoter 398..502 /gene="bla" /label="AmpR promoter" CDS 503..1363 /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" rep_origin 1534..2119 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" protein_bind 2407..2428 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" promoter 2443..2473 /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 2481..2497 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" primer_bind 2505..2521 /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" LTR 2650..3283 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 3490..4078 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" CDS complement(4092..4691) /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" promoter complement(4700..5029) /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS complement(5040..5786) /codon_start=1 /product="modified rtTA protein that binds tightly to promoters containing the tet operator in the presence of doxycycline" /transl_table=1 /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA DALDDFDLDMLPADALDDFDLDMLPG*" /label="Tet-On® 3G" promoter complement(5805..6315) /note="human phosphoglycerate kinase 1 promoter" /label="hPGK promoter" promoter 6332..6699 /note="3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On® 3G, modified to eliminate binding sites for endogenous mammalian transcription factors" /label="TRE3GS promoter" CDS 6712..7278 /label="REEP5(NM_005669)" /note="REEP5(NM_005669)" /gene="REEP5" CDS 7309..8016 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" /label="mCherry" CDS 8047..8766 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 8947..9081 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" misc_feature 9125..9240 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" CDS complement(9503..9547) /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 9732..9965 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" misc_feature 10462..10587 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" LTR 10634..11267 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" ORIGIN 1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaaact actaagtttg 61 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg 121 tttagagtaa atgaaaccac taggtaattg gcagtggtaa tagggtatgg ggtgggaagt 181 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg 241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga 361 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 1561 ctgctgcttg caaacaaaaa aaccgctacc agcggtggtt tgtttgccgg atcaagagct 1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 2161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 2221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 2281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 2341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 2401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 2461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 2521 ccatgattac gccaagctct agctagaggt cgacggtaaa acgctagcaa tgtcaaggcc 2581 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg 2641 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac 2701 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag 2761 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc 2821 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac 2881 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag 2941 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga 3001 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca 3061 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta 3121 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct 3181 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga 3241 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct cttttctttt 3301 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt 3361 gtgactggaa aacccacctc ctcctcctct tgtgcttcta gccaggcaca atcagcattg 3421 gtagctgctg tattgctact tgtgattgct ccatgttttt ctaggtctcg actgcagaat 3481 taattccagg cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa 3541 gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga 3601 gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga 3661 gcagccatgg aaaggacgtc agcttccccg acaacaccac ggaattgtca gtgcccaaca 3721 gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag 3781 ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag 3841 tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca 3901 actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac 3961 gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag 4021 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgt 4081 tccagacgcg ttcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc 4141 acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag gttgcggggc 4201 gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac tccggggagc 4261 acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac ggtggccagg 4321 aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg 4381 gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa caccgccccc 4441 gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac 4501 accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc 4561 tcgatgtggc ggtccgggtc gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg 4621 gcgagggtgc gtacggcccg ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg 4681 tactcggtca tggtcgcgtt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact 4741 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc 4801 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta 4861 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg 4921 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac 4981 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttcgcgcgtt 5041 tacccgggga gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg 5101 tcaaagtcgt caagggcatc ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg 5161 gtcggcccgc cgctttcgca ctttagctgt ttctccaggc cacatatgat tagttccagg 5221 ccgaaaagga aggcaggttc ggctccctgc cggtcgaaca gctcaattgc ttgtttcaga 5281 agtgggggca tagaatcggt ggtaggtgtc tctctttcct cttttgctac ttgatgctcc 5341 tgttcctcca atacgcagcc cagtgtaaag tggcccacgg cggacagagc gtacagtgcg 5401 ttctccaggg agaagccttg ctgacacagg aacgcgagct gattttccag ggtttcgtac 5461 tgtttctctg ttgggcgggt gccgagatgc actttagccc cgtcgcgatg tgagaggaga 5521 gcacagcggt atgacttggc gttgttccgc agaaagtctt gccatgactc gccttccagg 5581 gggcaggagt gggtatgatg cctgtccagc atctcgattg gcagggcatc gagcagggcc 5641 cgcttgttct tcacgtgcca gtacagggta ggctgctcaa ctcccagctt ttgagcgagt 5701 ttccttgtcg tcaggccttc gataccgact ccattgagta attccagagc agagtttatg 5761 actttgctct tgtccagtct agacatggta attcgatgat ccccctgggg agagaggtcg 5821 gtgattcggt caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg 5881 gaacaccgcg cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc 5941 gccccttccc ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc 6001 catcgcggtc ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg 6061 agacgtgcgg cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga 6121 cttaggggcg gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg 6181 gtgacgctgc gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc 6241 ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg 6301 cgcaacccca accccgtgga attatcacct cgagtttact ccctatcagt gatagagaac 6361 gtatgaagag tttactccct atcagtgata gagaacgtat gcagacttta ctccctatca 6421 gtgatagaga acgtataagg agtttactcc ctatcagtga tagagaacgt atgaccagtt 6481 tactccctat cagtgataga gaacgtatct acagtttact ccctatcagt gatagagaac 6541 gtatatccag tttactccct atcagtgata gagaacgtat aagctttgct tatgtaaacc 6601 agggcgccta taaaagagtg ctgatttttt gagtaaactt caattccaca acacttttgt 6661 cttataccaa ctttccgtac cacttcctac cctcgtaaag aattcgccac catgtctgcg 6721 gccatgaggg agaggttcga ccggttcctg cacgagaaga actgcatgac tgaccttctg 6781 gccaagctcg aggccaaaac cggcgtgaac aggagcttca tcgctcttgg tgtcatcgga 6841 ctggtggcct tgtacctggt gttcggttat ggagcctctc tcctctgcaa cctgatagga 6901 tttggctacc cagcctacat ctcaattaaa gctatagaga gtcccaacaa agaagatgat 6961 acccagtggc tgacctactg ggtagtgtat ggtgtgttca gcattgctga attcttctct 7021 gatatcttcc tgtcatggtt ccccttctac tacatgctga agtgtggctt cctgttgtgg 7081 tgcatggccc cgagcccttc taatggggct gaactgctct acaagcgcat catccgtcct 7141 ttcttcctga agcacgagtc ccagatggac agtgtggtca aggaccttaa agacaaggcc 7201 aaagagactg cagatgccat cactaaagaa gcgaagaaag ctaccgtgaa tttactgggt 7261 gaagaaaaga agagcaccgg cggtggagga agcggcggag gtggatctat ggtgagcaag 7321 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag 7381 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag 7441 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac 7501 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc 7561 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc 7621 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc 7681 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag 7741 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc 7801 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc 7861 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg 7921 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc 7981 cgccactcca ccggcggcat ggacgagctg tacaagggcg gtggaggaag cggcggaggt 8041 ggatctatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag 8101 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc 8161 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg 8221 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac 8281 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc 8341 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac 8401 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg 8461 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag 8521 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag 8581 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac 8641 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac 8701 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac 8761 aagtagggat ccagaccacc tcccctgcga gctaagctgg acagccaatg acgggtaaga 8821 gagtgacatt tttcactaac ctaagacagg agggccgtca gagctactgc ctaatccaaa 8881 gacgggtaaa agtgataaaa atgtatcact ccaacctaag acaggcgcag cttccgaggg 8941 atttgagatc cagacatgat aagatacatt gatgagtttg gacaaaccaa aactagaatg 9001 cagtgaaaaa aatgccttat ttgtgaaatt tgtgatgcta ttgccttatt tgtaaccatt 9061 ataagctgca ataaacaagt taagtcgata aactggatct ctgctgtccc tgtaataaac 9121 ccgaaaattt tgaatttttg taatttgttt ttgtagttct ttagtttgta tgtctgttgc 9181 tattatgtct actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa 9241 ggcgataccg tcgagatccg ttcactaatc gaatggatct gtctctgtct ctctctccac 9301 cttcttcttc tattccttcg ggcctgtcgg gtcccctcgg ggttgggagg tgggtctgaa 9361 acgataatgg tgaatatccc tgcctaactc tattcactat agaaagtaca gcaaaaacta 9421 ttcttaaacc taccaagcct cctactatca ttatgaataa ttttatatac cacagccaat 9481 ttgttatgtt aaaccaattc cacaaacttg cccatttatc taattccaat aattcttgtt 9541 cattcttttc ttgctggttt tgcgattctt caattaagga gtgtattaag cttgtgtaat 9601 tgttaatttc tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt tccagagatt 9661 tattactcca actagcattc caaggcacag cagtggtgca aatgagtttt ccagagcaac 9721 cccaaatccc caggagctgt tgatccttta ggtatctttc cacagccagg attcttgcct 9781 ggagctgctt gatgccccag actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc 9841 tcagcaaatt gttctgctgc tgcactatac cagacaataa ttgtctggcc tgtaccgtca 9901 gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc caagaaccca aggaacaaag 9961 ctcctattcc cactgctctt ttttctctct gcaccactct tctctttgcc ttggtgggtg 10021 ctactcctaa tggttcaatt tttactactt tatatttata taattcactt ctccaattgt 10081 ccctcatatc tcctcctcca ggtctgaaga tcagcggccg gccgcttgct gtgcggtggt 10141 cttacttttg ttttgctctt cctctatctt gtctaaagct tccttggtgt cttttatctc 10201 tatcctttga tgcacacaat agagggttgc tactgtatta tataatgatc taagttcttc 10261 tgatcctgtc tgaagggatg gttgtagctg tcccagtatt tgtctacagc cttctgatgt 10321 ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc ctgcttgccc atactatatg 10381 ttttaattta tattttttct ttccccctgg ccttaaccga attttttccc atcgcgatct 10441 aattctcccc cgcttaatac tgacgctctc gcacccatct ctctccttct agcctccgct 10501 agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc 10561 agcaagccga gtcctgcgtc gagagagctc ctctggtttc cctttcgctt tcaagtccct 10621 gttcgggcgc cactgctaga gattttccac actgactaaa agggtctgag ggatctctag 10681 ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat 10741 tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct 10801 aaccagagag acccagtaca ggcaaaaagc agctgcttat atgcaggatc tgagggctcg 10861 ccactcccca gtcccgccca ggccacgcct ccctggaaag tccccagcgg aaagtccctt 10921 gtagcaagct cgatatcagc agttcttgaa gtactccgga tgcagctctc gggccacgtg 10981 atgaaatgct aggcggctgt caaacctcca ctctaacact tctctctccg ggtcatccat 11041 cccatgcagg ctcacagggt gtaacaagct ggtgttctct cctttattgg cctcttctac 11101 cttatctggc tcaactggta ctagcttgta gcaccatcca aaggtcagtg gatatctgac 11161 ccctggccct ggtgtgtagt tctgctaatc agggaagtag ccttgtgtgt ggtagatcca 11221 cagatcaagg atatcttgtc ttctttggga gtgaattagc ccttcca //