LOCUS V036029 11645 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V036029 VERSION V036029 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers polyA_signal 216..350 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" promoter 398..502 /gene="bla" /label="AmpR promoter" CDS 503..1363 /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" rep_origin 1534..2119 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" protein_bind 2407..2428 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" promoter 2443..2473 /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 2481..2497 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" primer_bind 2505..2521 /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" LTR 2650..3283 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 3490..4078 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" CDS complement(4092..4691) /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" promoter complement(4700..5029) /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS complement(5040..5786) /codon_start=1 /product="modified rtTA protein that binds tightly to promoters containing the tet operator in the presence of doxycycline" /transl_table=1 /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA DALDDFDLDMLPADALDDFDLDMLPG*" /label="Tet-On® 3G" promoter complement(5805..6315) /note="human phosphoglycerate kinase 1 promoter" /label="hPGK promoter" promoter 6332..6699 /note="3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On® 3G, modified to eliminate binding sites for endogenous mammalian transcription factors" /label="TRE3GS promoter" CDS 6712..9141 /label="LRRC8A(NM_001127244)" /note="LRRC8A(NM_001127244)" /gene="LRRC8A" polyA_signal 9325..9459 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" misc_feature 9503..9618 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" CDS complement(9881..9925) /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 10110..10343 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" misc_feature 10840..10965 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" LTR 11012..11645 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" ORIGIN 1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaaact actaagtttg 61 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg 121 tttagagtaa atgaaaccac taggtaattg gcagtggtaa tagggtatgg ggtgggaagt 181 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg 241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga 361 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 1561 ctgctgcttg caaacaaaaa aaccgctacc agcggtggtt tgtttgccgg atcaagagct 1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 2161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 2221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 2281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 2341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 2401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 2461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 2521 ccatgattac gccaagctct agctagaggt cgacggtaaa acgctagcaa tgtcaaggcc 2581 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg 2641 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac 2701 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag 2761 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc 2821 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac 2881 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag 2941 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga 3001 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca 3061 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta 3121 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct 3181 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga 3241 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct cttttctttt 3301 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt 3361 gtgactggaa aacccacctc ctcctcctct tgtgcttcta gccaggcaca atcagcattg 3421 gtagctgctg tattgctact tgtgattgct ccatgttttt ctaggtctcg actgcagaat 3481 taattccagg cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa 3541 gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga 3601 gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga 3661 gcagccatgg aaaggacgtc agcttccccg acaacaccac ggaattgtca gtgcccaaca 3721 gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag 3781 ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag 3841 tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca 3901 actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac 3961 gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag 4021 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgt 4081 tccagacgcg ttcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc 4141 acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag gttgcggggc 4201 gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac tccggggagc 4261 acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac ggtggccagg 4321 aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg 4381 gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa caccgccccc 4441 gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac 4501 accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc 4561 tcgatgtggc ggtccgggtc gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg 4621 gcgagggtgc gtacggcccg ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg 4681 tactcggtca tggtcgcgtt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact 4741 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc 4801 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta 4861 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg 4921 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac 4981 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttcgcgcgtt 5041 tacccgggga gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg 5101 tcaaagtcgt caagggcatc ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg 5161 gtcggcccgc cgctttcgca ctttagctgt ttctccaggc cacatatgat tagttccagg 5221 ccgaaaagga aggcaggttc ggctccctgc cggtcgaaca gctcaattgc ttgtttcaga 5281 agtgggggca tagaatcggt ggtaggtgtc tctctttcct cttttgctac ttgatgctcc 5341 tgttcctcca atacgcagcc cagtgtaaag tggcccacgg cggacagagc gtacagtgcg 5401 ttctccaggg agaagccttg ctgacacagg aacgcgagct gattttccag ggtttcgtac 5461 tgtttctctg ttgggcgggt gccgagatgc actttagccc cgtcgcgatg tgagaggaga 5521 gcacagcggt atgacttggc gttgttccgc agaaagtctt gccatgactc gccttccagg 5581 gggcaggagt gggtatgatg cctgtccagc atctcgattg gcagggcatc gagcagggcc 5641 cgcttgttct tcacgtgcca gtacagggta ggctgctcaa ctcccagctt ttgagcgagt 5701 ttccttgtcg tcaggccttc gataccgact ccattgagta attccagagc agagtttatg 5761 actttgctct tgtccagtct agacatggta attcgatgat ccccctgggg agagaggtcg 5821 gtgattcggt caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg 5881 gaacaccgcg cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc 5941 gccccttccc ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc 6001 catcgcggtc ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg 6061 agacgtgcgg cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga 6121 cttaggggcg gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg 6181 gtgacgctgc gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc 6241 ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg 6301 cgcaacccca accccgtgga attatcacct cgagtttact ccctatcagt gatagagaac 6361 gtatgaagag tttactccct atcagtgata gagaacgtat gcagacttta ctccctatca 6421 gtgatagaga acgtataagg agtttactcc ctatcagtga tagagaacgt atgaccagtt 6481 tactccctat cagtgataga gaacgtatct acagtttact ccctatcagt gatagagaac 6541 gtatatccag tttactccct atcagtgata gagaacgtat aagctttgct tatgtaaacc 6601 agggcgccta taaaagagtg ctgatttttt gagtaaactt caattccaca acacttttgt 6661 cttataccaa ctttccgtac cacttcctac cctcgtaaag aattcgccac catgattccg 6721 gtgacagagc tccgctactt tgcggacacg cagccagcat accggatcct gaagccgtgg 6781 tgggatgtgt tcacagacta catctctatc gtcatgctga tgattgccgt cttcgggggg 6841 acgctgcagg tcacccaaga caagatgatc tgcctgcctt gtaagtgggt caccaaggac 6901 tcctgcaatg attcgttccg gggctgggca gcccctggcc cggagcccac ctaccccaac 6961 tccaccattc tgccgacccc tgacacgggc cccacaggca tcaagtatga cctggaccgg 7021 caccagtaca actacgtgga cgctgtgtgc tatgagaacc gactgcactg gtttgccaag 7081 tacttcccct acctggtgct tctgcacacg ctcatcttcc tggcctgcag caacttctgg 7141 ttcaaattcc cgcgcaccag ctcgaagctg gagcactttg tgtctatcct gctgaagtgc 7201 ttcgactcgc cctggaccac gagggccctg tcggagacag tggtggagga gagcgacccc 7261 aagccggcct tcagcaagat gaatgggtcc atggacaaaa agtcatcgac cgtcagtgag 7321 gacgtggagg ccaccgtgcc catgctgcag cggaccaagt cacggatcga gcagggtatc 7381 gtggaccgct cagagacggg cgtgctggac aagaaggagg gggagcaagc caaggcgctg 7441 tttgagaagg tgaagaagtt ccggacccat gtggaggagg gggacattgt gtaccgcctc 7501 tacatgcggc agaccatcat caaggtgatc aagttcatcc tcatcatctg ctacaccgtc 7561 tactacgtgc acaacatcaa gttcgacgtg gactgcaccg tggacattga gagcctgacg 7621 ggctaccgca cctaccgctg tgcccacccc ctggccacac tcttcaagat cctggcgtcc 7681 ttctacatca gcctagtcat cttctacggc ctcatctgca tgtatacact gtggtggatg 7741 ctacggcgct ccctcaagaa gtactcgttt gagtcgatcc gtgaggagag cagctacagc 7801 gacatccccg acgtcaagaa cgacttcgcc ttcatgctgc acctcattga ccaatacgac 7861 ccgctctact ccaagcgctt cgccgtcttc ctgtcggagg tgagtgagaa caagctgcgg 7921 cagctgaacc tcaacaacga gtggacgctg gacaagctcc ggcagcggct caccaagaac 7981 gcgcaggaca agctggagct gcacctgttc atgctcagtg gcatccctga cactgtgttt 8041 gacctggtgg agctggaggt cctcaagctg gagctgatcc ccgacgtgac catcccgccc 8101 agcattgccc agctcacggg cctcaaggag ctgtggctct accacacagc ggccaagatt 8161 gaagcgcccg cgctggcctt cctgcgtgag aacctgcggg cgctgcacat caagttcacc 8221 gacatcaagg agatcccgct gtggatctat agcctgaaga cactggagga gctgcacctg 8281 acgggcaacc tgagcgcgga gaacaaccgc tacatcgtca tcgacgggct gcgggagctc 8341 aaacgcctca aggtgctgcg gctcaagagc aacctaagca agctgccaca ggtggtcaca 8401 gatgtgggcg tgcacctgca gaagctgtcc atcaacaatg agggcaccaa gctcatcgtc 8461 ctcaacagcc tcaagaagat ggcgaacctg actgagctgg agctgatccg ctgtgacctg 8521 gagcgcatcc cccactccat cttcagcctc cacaacctgc aggagattga cctcaaggac 8581 aacaacctca agaccatcga ggagatcatc agcttccagc acctgcaccg cctcacctgc 8641 cttaagctgt ggtacaacca catcgcctac atccccatcc agatcggcaa cctcaccaac 8701 ctggagcgcc tctacctgaa ccgcaacaag atcgagaaga tccccaccca gctcttctac 8761 tgccgcaagc tgcgctacct ggacctcagc cacaacaacc tgaccttcct ccctgccgac 8821 atcggcctcc tgcagaacct ccagaaccta gccatcacgg ccaaccggat cgagacgctc 8881 cctccggagc tcttccagtg ccggaagctg cgggccctgc acctgggcaa caacgtgctg 8941 cagtcactgc cctccagggt gggcgagctg accaacctga cgcagatcga gctgcggggc 9001 aaccggctgg agtgcctgcc tgtggagctg ggcgagtgcc cactgctcaa gcgcagcggc 9061 ttggtggtgg aggaggacct gttcaacaca ctgccacccg aggtgaagga gcggctgtgg 9121 agggctgaca aggagcaggc ctgaggatcc agaccacctc ccctgcgagc taagctggac 9181 agccaatgac gggtaagaga gtgacatttt tcactaacct aagacaggag ggccgtcaga 9241 gctactgcct aatccaaaga cgggtaaaag tgataaaaat gtatcactcc aacctaagac 9301 aggcgcagct tccgagggat ttgagatcca gacatgataa gatacattga tgagtttgga 9361 caaaccaaaa ctagaatgca gtgaaaaaaa tgccttattt gtgaaatttg tgatgctatt 9421 gccttatttg taaccattat aagctgcaat aaacaagtta agtcgataaa ctggatctct 9481 gctgtccctg taataaaccc gaaaattttg aatttttgta atttgttttt gtagttcttt 9541 agtttgtatg tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat 9601 cccccctttt cttttaaagg cgataccgtc gagatccgtt cactaatcga atggatctgt 9661 ctctgtctct ctctccacct tcttcttcta ttccttcggg cctgtcgggt cccctcgggg 9721 ttgggaggtg ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag 9781 aaagtacagc aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt 9841 ttatatacca cagccaattt gttatgttaa accaattcca caaacttgcc catttatcta 9901 attccaataa ttcttgttca ttcttttctt gctggttttg cgattcttca attaaggagt 9961 gtattaagct tgtgtaattg ttaatttctc tgtcccactc catccaggtc gtgtgattcc 10021 aaatctgttc cagagattta ttactccaac tagcattcca aggcacagca gtggtgcaaa 10081 tgagttttcc agagcaaccc caaatcccca ggagctgttg atcctttagg tatctttcca 10141 cagccaggat tcttgcctgg agctgcttga tgccccagac tgtgagttgc aacagatgct 10201 gttgcgcctc aatagccctc agcaaattgt tctgctgctg cactatacca gacaataatt 10261 gtctggcctg taccgtcagc gtcattgacg ctgcgcccat agtgcttcct gctgctccca 10321 agaacccaag gaacaaagct cctattccca ctgctctttt ttctctctgc accactcttc 10381 tctttgcctt ggtgggtgct actcctaatg gttcaatttt tactacttta tatttatata 10441 attcacttct ccaattgtcc ctcatatctc ctcctccagg tctgaagatc agcggccggc 10501 cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc 10561 cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta ctgtattata 10621 taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc ccagtatttg 10681 tctacagcct tctgatgttt ctaacaggcc aggattaact gcgaatcgtt ctagctccct 10741 gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc ttaaccgaat 10801 tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc acccatctct 10861 ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc 10921 tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct ctggtttccc 10981 tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac tgactaaaag 11041 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc 11101 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc 11161 caggctcaga tctggtctaa ccagagagac ccagtacagg caaaaagcag ctgcttatat 11221 gcaggatctg agggctcgcc actccccagt cccgcccagg ccacgcctcc ctggaaagtc 11281 cccagcggaa agtcccttgt agcaagctcg atatcagcag ttcttgaagt actccggatg 11341 cagctctcgg gccacgtgat gaaatgctag gcggctgtca aacctccact ctaacacttc 11401 tctctccggg tcatccatcc catgcaggct cacagggtgt aacaagctgg tgttctctcc 11461 tttattggcc tcttctacct tatctggctc aactggtact agcttgtagc accatccaaa 11521 ggtcagtgga tatctgaccc ctggccctgg tgtgtagttc tgctaatcag ggaagtagcc 11581 ttgtgtgtgg tagatccaca gatcaaggat atcttgtctt ctttgggagt gaattagccc 11641 ttcca //