LOCUS       V022484                11324 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022484
VERSION     V022484
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     polyA_signal    216..350
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     promoter        398..502
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             503..1363
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      1534..2119
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     protein_bind    2407..2428
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        2443..2473
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2481..2497
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     2505..2521
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     LTR             2650..3283
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    3491..4079
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     CDS             complement(4093..4692)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     promoter        complement(4701..5030)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             complement(5041..5787)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to
                     promoters containing the tet operator in the presence of
                     doxycycline"
                     /transl_table=1
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG*"
                     /label="Tet-On® 3G"
     promoter        complement(5806..6316)
                     /note="human phosphoglycerate kinase 1 promoter"
                     /label="hPGK promoter"
     promoter        6333..6700
                     /note="3rd-generation Tet-responsive promoter that can be
                     activated by binding of Tet-On® 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription
                     factors"
                     /label="TRE3GS promoter"
     CDS             6713..7429
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             7445..8791
                     /label="HNRNPH2(NM_001032393)"
                     /note="HNRNPH2(NM_001032393)"
                     /gene="HNRNPH2"
     CDS             8792..8821
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     polyA_signal    9004..9138
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     misc_feature    9182..9297
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     CDS             complement(9560..9604)
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    9789..10022
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     misc_feature    10519..10644
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     LTR             10691..11324
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
ORIGIN
        1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaaact actaagtttg
       61 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg
      121 tttagagtaa atgaaaccac taggtaattg gcagtggtaa tagggtatgg ggtgggaagt
      181 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg
      241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
      301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga
      361 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
      721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
      781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
      901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
      961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1561 ctgctgcttg caaacaaaaa aaccgctacc agcggtggtt tgtttgccgg atcaagagct
     1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     2401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     2461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     2521 ccatgattac gccaagctct agctagaggt cgacggtaaa acgctagcaa tgtcaaggcc
     2581 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg
     2641 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac
     2701 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag
     2761 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc
     2821 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac
     2881 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag
     2941 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga
     3001 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca
     3061 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta
     3121 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct
     3181 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga
     3241 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct cttttctttt
     3301 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt
     3361 gtgactggaa aaacccacct cctcctcctc ttgtgcttct agccaggcac aatcagcatt
     3421 ggtagctgct gtattgctac ttgtgattgc tccatgtttt tctaggtctc gactgcagaa
     3481 ttaattccag gcggggaggc ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga
     3541 agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc cgctggattg
     3601 agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg
     3661 agcagccatg gaaaggacgt cagcttcccc gacaacacca cggaattgtc agtgcccaac
     3721 agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc cgtggcaata
     3781 gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat gccccaacca
     3841 gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac aacgggccac
     3901 aactcctcat aaagagacag caaccaggat ttatacaagg aggagaaaat gaaagccata
     3961 cgggaagcaa tagcatgata caaaggcatt aaagcagcgt atccacatag cgtaaaagga
     4021 gcaacatagt taagaatacc agtcaatctt tcacaaattt tgtaatccag aggttgattg
     4081 ttccagacgc gttcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg
     4141 cacctcgacg tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg
     4201 cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag
     4261 cacgacggcg ctgcccagac ccttgccctg gtggtcgggc gagacgccga cggtggccag
     4321 gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc
     4381 ggccagccgg gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc
     4441 cgcttcgacg ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca
     4501 caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg
     4561 ctcgatgtgg cggtccgggt cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc
     4621 ggcgagggtg cgtacggccc gggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt
     4681 gtactcggtc atggtcgcgt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac
     4741 ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt
     4801 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt
     4861 aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     4921 ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata
     4981 cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac attcgcgcgt
     5041 ttacccgggg agcatgtcaa ggtcaaaatc gtcaagagcg tcagcaggca gcatatcaag
     5101 gtcaaagtcg tcaagggcat cggctgggag catgtctaag tcaaaatcgt caagggcgtc
     5161 ggtcggcccg ccgctttcgc actttagctg tttctccagg ccacatatga ttagttccag
     5221 gccgaaaagg aaggcaggtt cggctccctg ccggtcgaac agctcaattg cttgtttcag
     5281 aagtgggggc atagaatcgg tggtaggtgt ctctctttcc tcttttgcta cttgatgctc
     5341 ctgttcctcc aatacgcagc ccagtgtaaa gtggcccacg gcggacagag cgtacagtgc
     5401 gttctccagg gagaagcctt gctgacacag gaacgcgagc tgattttcca gggtttcgta
     5461 ctgtttctct gttgggcggg tgccgagatg cactttagcc ccgtcgcgat gtgagaggag
     5521 agcacagcgg tatgacttgg cgttgttccg cagaaagtct tgccatgact cgccttccag
     5581 ggggcaggag tgggtatgat gcctgtccag catctcgatt ggcagggcat cgagcagggc
     5641 ccgcttgttc ttcacgtgcc agtacagggt aggctgctca actcccagct tttgagcgag
     5701 tttccttgtc gtcaggcctt cgataccgac tccattgagt aattccagag cagagtttat
     5761 gactttgctc ttgtccagtc tagacatggt aattcgatga tccccctggg gagagaggtc
     5821 ggtgattcgg tcaacgaggg agccgactgc cgacgtgcgc tccggaggct tgcagaatgc
     5881 ggaacaccgc gcgggcagga acagggccca cactaccgcc ccacaccccg cctcccgcac
     5941 cgccccttcc cggccgctgc tctcggcgcg ccctgctgag cagccgctat tggccacagc
     6001 ccatcgcggt cggcgcgctg ccattgctcc ctggcgctgt ccgtctgcga gggtactagt
     6061 gagacgtgcg gcttccgttt gtcacgtccg gcacgccgcg aaccgcaagg aaccttcccg
     6121 acttaggggc ggagcaggaa gcgtcgccgg ggggcccaca agggtagcgg cgaagatccg
     6181 ggtgacgctg cgaacggacg tgaagaatgt gcgagaccca gggtcggcgc cgctgcgttt
     6241 cccggaacca cgcccagagc agccgcgtcc ctgcgcaaac ccagggctgc cttggaaaag
     6301 gcgcaacccc aaccccgtgg aattatcacc tcgagtttac tccctatcag tgatagagaa
     6361 cgtatgaaga gtttactccc tatcagtgat agagaacgta tgcagacttt actccctatc
     6421 agtgatagag aacgtataag gagtttactc cctatcagtg atagagaacg tatgaccagt
     6481 ttactcccta tcagtgatag agaacgtatc tacagtttac tccctatcag tgatagagaa
     6541 cgtatatcca gtttactccc tatcagtgat agagaacgta taagctttgc ttatgtaaac
     6601 cagggcgcct ataaaagagt gctgattttt tgagtaaact tcaattccac aacacttttg
     6661 tcttatacca actttccgta ccacttccta ccctcgtaaa gaattcgcca ccatggtgag
     6721 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     6781 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     6841 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     6901 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     6961 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     7021 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     7081 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     7141 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     7201 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     7261 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     7321 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     7381 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg gaggaggggg
     7441 aagcatgatg ctgagcacgg aaggcaggga ggggttcgtg gtgaaggtca ggggcctacc
     7501 ctggtcctgc tcagccgatg aagtgatgcg cttcttctct gattgcaaga tccaaaatgg
     7561 cacatcaggt attcgtttca tctacaccag agaaggcaga ccaagtggtg aagcatttgt
     7621 tgaacttgaa tctgaagagg aagtgaaatt ggctttgaag aaggacagag aaaccatggg
     7681 acacagatac gttgaagtat tcaagtctaa cagtgttgaa atggattggg tgttgaagca
     7741 tacaggtccg aatagccctg atactgccaa cgatggcttc gtccggctta gaggactccc
     7801 atttggctgt agcaaggaag agattgttca gttcttttca gggttggaaa ttgtgccaaa
     7861 tgggatgaca ctgccagtgg actttcaggg gcgaagcaca ggggaagcct ttgtgcagtt
     7921 tgcttcacag gagatagctg agaaggcctt aaagaaacac aaggaaagaa tagggcacag
     7981 gtacattgag atcttcaaga gtagccgagc tgaagttcga acccactatg atccccctcg
     8041 aaagctcatg gctatgcagc ggccaggtcc ctatgatagg ccgggggctg gcagagggta
     8101 taatagcatt ggcagaggag ctgggtttga aaggatgagg cgtggtgcct atggtggagg
     8161 gtatggaggc tatgatgact atggtggcta taatgatgga tatggctttg ggtctgatag
     8221 atttggaaga gacctcaatt actgtttttc aggaatgtct gatcatagat acggagatgg
     8281 tgggtccagt ttccagagca ccacagggca ctgtgtacac atgagggggt taccttacag
     8341 agccactgag aatgatattt ataatttctt ctcacctctt aatcccatga gagtacatat
     8401 tgaaattgga cccgatggca gagttaccgg tgaggcagat gttgaatttg ctactcatga
     8461 agatgctgtg gcagctatgg caaaagacaa agctaatatg caacacagat atgtggagct
     8521 cttcttaaat tctactgcag gaacaagtgg gggtgcttac gatcacagct atgtagaact
     8581 ttttttgaat tctacagcag gggcaagtgg tggcgcttat ggtagccaaa tgatgggagg
     8641 gatgggctta tccaaccagt ctagttatgg aggtcctgct agccagcagc tgagtggtgg
     8701 ttatggaggt ggttatggtg gtcagagcag tatgagtgga tatgaccaag ttctgcagga
     8761 aaactccagt gactatcagt caaaccttgc tgagcaaaag ctcatttctg aagaggactt
     8821 gtaggatcca gaccacctcc cctgcgagct aagctggaca gccaatgacg ggtaagagag
     8881 tgacattttt cactaaccta agacaggagg gccgtcagag ctactgccta atccaaagac
     8941 gggtaaaagt gataaaaatg tatcactcca acctaagaca ggcgcagctt ccgagggatt
     9001 tgagatccag acatgataag atacattgat gagtttggac aaaccaaaac tagaatgcag
     9061 tgaaaaaaat gccttatttg tgaaatttgt gatgctattg ccttatttgt aaccattata
     9121 agctgcaata aacaagttaa gtcgataaac tggatctctg ctgtccctgt aataaacccg
     9181 aaaattttga atttttgtaa tttgtttttg tagttcttta gtttgtatgt ctgttgctat
     9241 tatgtctact attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaggc
     9301 gataccgtcg agatccgttc actaatcgaa tggatctgtc tctgtctctc tctccacctt
     9361 cttcttctat tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg
     9421 ataatggtga atatccctgc ctaactctat tcactataga aagtacagca aaaactattc
     9481 ttaaacctac caagcctcct actatcatta tgaataattt tatataccac agccaatttg
     9541 ttatgttaaa ccaattccac aaacttgccc atttatctaa ttccaataat tcttgttcat
     9601 tcttttcttg ctggttttgc gattcttcaa ttaaggagtg tattaagctt gtgtaattgt
     9661 taatttctct gtcccactcc atccaggtcg tgtgattcca aatctgttcc agagatttat
     9721 tactccaact agcattccaa ggcacagcag tggtgcaaat gagttttcca gagcaacccc
     9781 aaatccccag gagctgttga tcctttaggt atctttccac agccaggatt cttgcctgga
     9841 gctgcttgat gccccagact gtgagttgca acagatgctg ttgcgcctca atagccctca
     9901 gcaaattgtt ctgctgctgc actataccag acaataattg tctggcctgt accgtcagcg
     9961 tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg aacaaagctc
    10021 ctattcccac tgctcttttt tctctctgca ccactcttct ctttgccttg gtgggtgcta
    10081 ctcctaatgg ttcaattttt actactttat atttatataa ttcacttctc caattgtccc
    10141 tcatatctcc tcctccaggt ctgaagatca gcggccggcc gcttgctgtg cggtggtctt
    10201 acttttgttt tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt ttatctctat
    10261 cctttgatgc acacaataga gggttgctac tgtattatat aatgatctaa gttcttctga
    10321 tcctgtctga agggatggtt gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc
    10381 taacaggcca ggattaactg cgaatcgttc tagctccctg cttgcccata ctatatgttt
    10441 taatttatat tttttctttc cccctggcct taaccgaatt ttttcccatc gcgatctaat
    10501 tctcccccgc ttaatactga cgctctcgca cccatctctc tccttctagc ctccgctagt
    10561 caaaattttt ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc
    10621 aagccgagtc ctgcgtcgag agagctcctc tggtttccct ttcgctttca agtccctgtt
    10681 cgggcgccac tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta
    10741 ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga
    10801 ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac
    10861 cagagagacc cagtacaggc aaaaagcagc tgcttatatg caggatctga gggctcgcca
    10921 ctccccagtc ccgcccaggc cacgcctccc tggaaagtcc ccagcggaaa gtcccttgta
    10981 gcaagctcga tatcagcagt tcttgaagta ctccggatgc agctctcggg ccacgtgatg
    11041 aaatgctagg cggctgtcaa acctccactc taacacttct ctctccgggt catccatccc
    11101 atgcaggctc acagggtgta acaagctggt gttctctcct ttattggcct cttctacctt
    11161 atctggctca actggtacta gcttgtagca ccatccaaag gtcagtggat atctgacccc
    11221 tggccctggt gtgtagttct gctaatcagg gaagtagcct tgtgtgtggt agatccacag
    11281 atcaaggata tcttgtcttc tttgggagtg aattagccct tcca
//
