LOCUS       V032362                12179 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032362
VERSION     V032362
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     polyA_signal    216..350
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     promoter        398..502
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             503..1363
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      1534..2119
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     protein_bind    2407..2428
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        2443..2473
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2481..2497
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     2505..2521
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     LTR             2650..3283
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    3490..4078
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     CDS             complement(4092..4691)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     promoter        complement(4700..5029)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             complement(5040..5786)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to
                     promoters containing the tet operator in the presence of
                     doxycycline"
                     /transl_table=1
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG*"
                     /label="Tet-On® 3G"
     promoter        complement(5805..6315)
                     /note="human phosphoglycerate kinase 1 promoter"
                     /label="hPGK promoter"
     promoter        6332..6699
                     /note="3rd-generation Tet-responsive promoter that can be
                     activated by binding of Tet-On® 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription
                     factors"
                     /label="TRE3GS promoter"
     CDS             6712..8661
                     /label="FAF1(NM_007051)"
                     /note="FAF1(NM_007051)"
                     /gene="FAF1"
     CDS             8719..9675
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID
                     engineered for improved catalytic efficiency (Branon et
                     al., 2018)"
                     /transl_table=1
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
                     /label="TurboID"
     polyA_signal    9859..9993
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     misc_feature    10037..10152
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     CDS             complement(10415..10459)
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    10644..10877
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     misc_feature    11374..11499
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     LTR             11546..12179
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
ORIGIN
        1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaaact actaagtttg
       61 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg
      121 tttagagtaa atgaaaccac taggtaattg gcagtggtaa tagggtatgg ggtgggaagt
      181 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg
      241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
      301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga
      361 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
      721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
      781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
      901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
      961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1561 ctgctgcttg caaacaaaaa aaccgctacc agcggtggtt tgtttgccgg atcaagagct
     1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     2401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     2461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     2521 ccatgattac gccaagctct agctagaggt cgacggtaaa acgctagcaa tgtcaaggcc
     2581 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg
     2641 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac
     2701 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag
     2761 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc
     2821 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac
     2881 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag
     2941 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga
     3001 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca
     3061 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta
     3121 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct
     3181 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga
     3241 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct cttttctttt
     3301 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt
     3361 gtgactggaa aacccacctc ctcctcctct tgtgcttcta gccaggcaca atcagcattg
     3421 gtagctgctg tattgctact tgtgattgct ccatgttttt ctaggtctcg actgcagaat
     3481 taattccagg cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa
     3541 gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga
     3601 gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga
     3661 gcagccatgg aaaggacgtc agcttccccg acaacaccac ggaattgtca gtgcccaaca
     3721 gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag
     3781 ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag
     3841 tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca
     3901 actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac
     3961 gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag
     4021 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgt
     4081 tccagacgcg ttcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc
     4141 acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag gttgcggggc
     4201 gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac tccggggagc
     4261 acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac ggtggccagg
     4321 aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg
     4381 gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa caccgccccc
     4441 gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac
     4501 accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc
     4561 tcgatgtggc ggtccgggtc gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg
     4621 gcgagggtgc gtacggcccg ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg
     4681 tactcggtca tggtcgcgtt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact
     4741 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc
     4801 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
     4861 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     4921 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac
     4981 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttcgcgcgtt
     5041 tacccgggga gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg
     5101 tcaaagtcgt caagggcatc ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg
     5161 gtcggcccgc cgctttcgca ctttagctgt ttctccaggc cacatatgat tagttccagg
     5221 ccgaaaagga aggcaggttc ggctccctgc cggtcgaaca gctcaattgc ttgtttcaga
     5281 agtgggggca tagaatcggt ggtaggtgtc tctctttcct cttttgctac ttgatgctcc
     5341 tgttcctcca atacgcagcc cagtgtaaag tggcccacgg cggacagagc gtacagtgcg
     5401 ttctccaggg agaagccttg ctgacacagg aacgcgagct gattttccag ggtttcgtac
     5461 tgtttctctg ttgggcgggt gccgagatgc actttagccc cgtcgcgatg tgagaggaga
     5521 gcacagcggt atgacttggc gttgttccgc agaaagtctt gccatgactc gccttccagg
     5581 gggcaggagt gggtatgatg cctgtccagc atctcgattg gcagggcatc gagcagggcc
     5641 cgcttgttct tcacgtgcca gtacagggta ggctgctcaa ctcccagctt ttgagcgagt
     5701 ttccttgtcg tcaggccttc gataccgact ccattgagta attccagagc agagtttatg
     5761 actttgctct tgtccagtct agacatggta attcgatgat ccccctgggg agagaggtcg
     5821 gtgattcggt caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg
     5881 gaacaccgcg cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc
     5941 gccccttccc ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc
     6001 catcgcggtc ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg
     6061 agacgtgcgg cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga
     6121 cttaggggcg gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg
     6181 gtgacgctgc gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc
     6241 ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg
     6301 cgcaacccca accccgtgga attatcacct cgagtttact ccctatcagt gatagagaac
     6361 gtatgaagag tttactccct atcagtgata gagaacgtat gcagacttta ctccctatca
     6421 gtgatagaga acgtataagg agtttactcc ctatcagtga tagagaacgt atgaccagtt
     6481 tactccctat cagtgataga gaacgtatct acagtttact ccctatcagt gatagagaac
     6541 gtatatccag tttactccct atcagtgata gagaacgtat aagctttgct tatgtaaacc
     6601 agggcgccta taaaagagtg ctgatttttt gagtaaactt caattccaca acacttttgt
     6661 cttataccaa ctttccgtac cacttcctac cctcgtaaag aattcgccac catggcgtcc
     6721 aacatggacc gggagatgat cctggcggat tttcaggcat gtactggcat tgaaaacatt
     6781 gacgaagcta ttacattgct tgaacaaaat aattgggact tagtggcagc tatcaatggt
     6841 gtaataccac aggaaaatgg cattctacaa agtgaatatg gaggtgagac cataccagga
     6901 cctgcattta atccagcaag tcatccagct tcagctccta cttcctcttc ttcttcagcg
     6961 tttcgacctg taatgccatc caggcagatt gtagaaaggc aacctcggat gctggacttc
     7021 agggttgaat acagagacag aaatgttgat gtggtacttg aagacacctg tactgttgga
     7081 gagattaaac agattctaga aaatgaactt cagatacctg tgtccaaaat gctgttaaaa
     7141 ggctggaaga cgggagatgt ggaagacagt acggtcctaa aatctctaca cttgccaaaa
     7201 aacaacagtc tttatgtcct tacaccagat ttgccaccac cttcatcatc tagtcatgct
     7261 ggtgccctgc aggagtcatt aaatcaaaac ttcatgctga tcatcaccca ccgagaagtc
     7321 cagcgggagt acaacctgaa cttctcagga agcagtacta ttcaagaggt aaagagaaat
     7381 gtgtatgacc ttacaagtat ccccgttcgc caccaattat gggagggctg gccaacttct
     7441 gctacagacg actcaatgtg tcttgctgaa tcagggctct cttatccctg ccatcgactt
     7501 acagtgggaa gaagatcttc acctgcacag acccgggaac agtcggaaga acaaatcacc
     7561 gatgttcata tggttagtga tagcgatgga gatgactttg aagatgctac agaatttggg
     7621 gtggatgatg gagaagtatt tggcatggcg tcatctgcct tgagaaaatc tccaatgatg
     7681 ccagaaaacg cagaaaatga aggagatgcc ttattacaat ttacagcaga gttttcttca
     7741 agatatggtg attgccatcc tgtatttttt attggctcat tagaagctgc ttttcaagag
     7801 gccttctatg tgaaagcccg agatagaaag cttcttgcta tctacctcca ccatgatgaa
     7861 agtgtgttaa ccaacgtgtt ctgctcacaa atgctttgtg ctgaatccat tgtttcttat
     7921 ctgagtcaaa attttataac ctgggcttgg gatctgacaa aggactccaa cagagcaaga
     7981 tttctcacta tgtgcaatag acactttggc agtgttgtgg cacaaaccat tcggactcaa
     8041 aaaacggatc agtttccgct tttcctgatt attatgggaa agcgatcatc taatgaagtg
     8101 ttgaatgtga tacaagggaa cacaacagta gatgagttaa tgatgagact catggctgca
     8161 atggagatct tcacagccca acaacaggaa gatataaagg acgaggatga acgtgaagcc
     8221 agagaaaatg tgaagagaga gcaagatgag gcctatcgcc tttcacttga ggctgacaga
     8281 gcaaagaggg aagctcacga gagagagatg gcagaacagt ttcgtttgga gcagattcgc
     8341 aaagaacaag aagaggaacg tgaggccatc cggctgtcct tagagcaagc cctgcctcct
     8401 gagccaaagg aagaaaatgc tgagcctgtg agcaaactgc ggatccggac ccccagtggc
     8461 gagttcttgg agcggcgttt cctggccagc aacaagctcc agattgtctt tgattttgta
     8521 gcttccaaag gatttccatg ggatgagtac aagttactga gcacctttcc taggagagac
     8581 gtaactcaac tggacccaaa taaatcatta ttggaggtaa agttgttccc tcaagaaacc
     8641 cttttccttg aagcaaaaga gtgtacaggc ggcggcggca gcggcggcgg cggcagcggc
     8701 ggcggcggca gcgctagcaa agacaatact gtgcctctga agctgatcgc tctcctggct
     8761 aatggcgagt tccatagtgg cgaacagctg ggagaaaccc tgggcatgtc cagggccgct
     8821 atcaacaagc acattcagac tctgcgcgac tggggcgtgg acgtgttcac cgtgcccgga
     8881 aagggctact ctctgcccga gcctatcccg ctgctgaacg ctaaacagat tctgggacag
     8941 ctggacggcg ggagcgtggc agtcctgcct gtggtcgact ccaccaatca gtacctgctg
     9001 gatcgaatcg gcgagctgaa gagtggggat gcttgcattg cagaatatca gcaggcaggg
     9061 agaggaagca gagggaggaa atggttctct ccttttggag ctaacctgta cctgagtatg
     9121 ttttggcgcc tgaagcgggg accagcagca atcggcctgg gcccggtcat cggaattgtc
     9181 atggcagaag cgctgcgaaa gctgggagca gacaaggtgc gagtcaaatg gcccaatgac
     9241 ctgtatctgc aggatagaaa gctggcaggc atcctggtgg agctggccgg aataacaggc
     9301 gatgctgcac agatcgtcat tggcgccggg attaacgtgg ctatgaggcg cgtggaggaa
     9361 agcgtggtca atcagggctg gatcacactg caggaagcag ggattaacct ggacaggaat
     9421 actctggccg ctacgctgat ccgagagctg cgggcagccc tggaactgtt cgagcaggaa
     9481 ggcctggctc catatctgcc acggtgggag aagctggata acttcatcaa tagacccgtg
     9541 aagctgatca ttggggacaa agagattttc gggattagcc gggggattga taaacaggga
     9601 gccctgctgc tggaacagga cggagttatc aaaccctgga tgggcggaga aatcagtctg
     9661 cggtctgccg aaaagtaggg atccagacca cctcccctgc gagctaagct ggacagccaa
     9721 tgacgggtaa gagagtgaca tttttcacta acctaagaca ggagggccgt cagagctact
     9781 gcctaatcca aagacgggta aaagtgataa aaatgtatca ctccaaccta agacaggcgc
     9841 agcttccgag ggatttgaga tccagacatg ataagataca ttgatgagtt tggacaaacc
     9901 aaaactagaa tgcagtgaaa aaaatgcctt atttgtgaaa tttgtgatgc tattgcctta
     9961 tttgtaacca ttataagctg caataaacaa gttaagtcga taaactggat ctctgctgtc
    10021 cctgtaataa acccgaaaat tttgaatttt tgtaatttgt ttttgtagtt ctttagtttg
    10081 tatgtctgtt gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc
    10141 ttttctttta aaggcgatac cgtcgagatc cgttcactaa tcgaatggat ctgtctctgt
    10201 ctctctctcc accttcttct tctattcctt cgggcctgtc gggtcccctc ggggttggga
    10261 ggtgggtctg aaacgataat ggtgaatatc cctgcctaac tctattcact atagaaagta
    10321 cagcaaaaac tattcttaaa cctaccaagc ctcctactat cattatgaat aattttatat
    10381 accacagcca atttgttatg ttaaaccaat tccacaaact tgcccattta tctaattcca
    10441 ataattcttg ttcattcttt tcttgctggt tttgcgattc ttcaattaag gagtgtatta
    10501 agcttgtgta attgttaatt tctctgtccc actccatcca ggtcgtgtga ttccaaatct
    10561 gttccagaga tttattactc caactagcat tccaaggcac agcagtggtg caaatgagtt
    10621 ttccagagca accccaaatc cccaggagct gttgatcctt taggtatctt tccacagcca
    10681 ggattcttgc ctggagctgc ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg
    10741 cctcaatagc cctcagcaaa ttgttctgct gctgcactat accagacaat aattgtctgg
    10801 cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct cccaagaacc
    10861 caaggaacaa agctcctatt cccactgctc ttttttctct ctgcaccact cttctctttg
    10921 ccttggtggg tgctactcct aatggttcaa tttttactac tttatattta tataattcac
    10981 ttctccaatt gtccctcata tctcctcctc caggtctgaa gatcagcggc cggccgcttg
    11041 ctgtgcggtg gtcttacttt tgttttgctc ttcctctatc ttgtctaaag cttccttggt
    11101 gtcttttatc tctatccttt gatgcacaca atagagggtt gctactgtat tatataatga
    11161 tctaagttct tctgatcctg tctgaaggga tggttgtagc tgtcccagta tttgtctaca
    11221 gccttctgat gtttctaaca ggccaggatt aactgcgaat cgttctagct ccctgcttgc
    11281 ccatactata tgttttaatt tatatttttt ctttccccct ggccttaacc gaattttttc
    11341 ccatcgcgat ctaattctcc cccgcttaat actgacgctc tcgcacccat ctctctcctt
    11401 ctagcctccg ctagtcaaaa tttttggcgt actcaccagt cgccgcccct cgcctcttgc
    11461 cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt tccctttcgc
    11521 tttcaagtcc ctgttcgggc gccactgcta gagattttcc acactgacta aaagggtctg
    11581 agggatctct agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa
    11641 ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct
    11701 cagatctggt ctaaccagag agacccagta caggcaaaaa gcagctgctt atatgcagga
    11761 tctgagggct cgccactccc cagtcccgcc caggccacgc ctccctggaa agtccccagc
    11821 ggaaagtccc ttgtagcaag ctcgatatca gcagttcttg aagtactccg gatgcagctc
    11881 tcgggccacg tgatgaaatg ctaggcggct gtcaaacctc cactctaaca cttctctctc
    11941 cgggtcatcc atcccatgca ggctcacagg gtgtaacaag ctggtgttct ctcctttatt
    12001 ggcctcttct accttatctg gctcaactgg tactagcttg tagcaccatc caaaggtcag
    12061 tggatatctg acccctggcc ctggtgtgta gttctgctaa tcagggaagt agccttgtgt
    12121 gtggtagatc cacagatcaa ggatatcttg tcttctttgg gagtgaatta gcccttcca
//
