LOCUS       V013359                 8627 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013359
VERSION     V013359
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8627)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2201..2530
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     misc_feature    2637..3223
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             3225..3917
                     /label="TurboRFP"
                     /note="red fluorescent protein from Entacmaea quadricolor"
     CDS             3936..4532
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    4549..5137
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5344..5977
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6105..6121)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6129..6145)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6153..6183)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6198..6219)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6507..7095)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7269..8126)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8127..8231)
                     /label="AmpR promoter"
     polyA_signal    8279..8413
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg gtgtgtcagt tagggtgtgg
     2221 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     2281 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     2341 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     2401 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     2461 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     2521 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
     2581 atgaggatcg tttcgcgaat tcctcgagac tagttctaga gcggccgcgg atcccgcccc
     2641 tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     2701 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     2761 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     2821 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     2881 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     2941 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     3001 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     3061 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
     3121 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
     3181 gtggttttcc tttgaaaaac acgatgataa gcttgccaca acccatgagc gagctgatca
     3241 aggagaacat gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt
     3301 gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaag atcaaggtgg
     3361 tcgagggcgg ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca
     3421 gcaaagcctt catcaaccac acccagggca tccccgactt ctttaagcag tccttccctg
     3481 agggcttcac atgggagaga atcaccacat acgaagacgg gggcgtgctg accgctaccc
     3541 aggacaccag cttccagaac ggctgcatca tctacaacgt caagatcaac ggggtgaact
     3601 tcccatccaa cggccctgtg atgcagaaga aaacacgcgg ctgggaggcc aacaccgaga
     3661 tgctgtaccc cgctgacggc ggcctgagag gccacagcca gatggccctg aagctcgtgg
     3721 gcgggggcta cctgcactgc tccttcaaga ccacatacag atccaagaaa cccgctaaga
     3781 acctcaagat gcccggcttc cacttcgtgg accacagact ggaaagaatc aaggaggccg
     3841 acaaagagac ctacgtcgag cagcacgaga tggctgtggc caagtactgc gacctcccta
     3901 gcaaactggg gcacagaaca aggagacgac cttccatgac cgagtacaag cccacggtgc
     3961 gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg
     4021 actaccccgc cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc
     4081 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     4141 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     4201 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     4261 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     4321 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     4381 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     4441 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     4501 gcacctggtg catgacccgc aagcccggtg cctagacgcg tctggaacaa tcaacctctg
     4561 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     4621 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     4681 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     4741 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     4801 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     4861 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     4921 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     4981 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     5041 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     5101 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag
     5161 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     5221 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     5281 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg
     5341 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     5401 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
     5461 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
     5521 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
     5581 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
     5641 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
     5701 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
     5761 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
     5821 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     5881 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     5941 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     6001 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattgc
     6061 tagcgtttac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     6121 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     6181 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     6241 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     6301 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     6361 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     6421 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6481 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6541 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6601 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6661 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6721 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6781 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6841 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6901 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     6961 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7021 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7081 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7141 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7201 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7261 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7321 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7381 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7441 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7501 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7561 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7621 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7681 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7741 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7801 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7861 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7921 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7981 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8041 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8101 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8161 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8221 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa
     8281 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     8341 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     8401 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
     8461 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc
     8521 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
     8581 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagt
//
