LOCUS       V021219                 9968 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021219
VERSION     V021219
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2826..4694
                     /label="ZNF131(NM_001330707)"
                     /note="ZNF131(NM_001330707)"
                     /gene="ZNF131"
     CDS             4695..4724
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     promoter        4756..5255
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5276..5875
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5889..6477
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6684..7317
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7446..7462)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7470..7486
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7494..7524)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7539..7560
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7848..8436)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8607..9467)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9468..9572)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    9620..9754
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ccaccatgga ggctgaagag acgatggaat gccttcagga gttccctgaa catcataaaa
     2881 tgatcctcga ccgattgaat gaacagcgag agcaggaccg gtttactgac atcaccctaa
     2941 ttgtcgacgg acaccatttt aaggctcaca aggctgtttt ggctgcttgt agtaagttct
     3001 tctacaaatt ctttcaggag tttacccaag aaccattggt ggagatagaa ggtgttagta
     3061 aaatggcctt tcgccattta attgagttca catatacagc aaaattaatg atacaaggag
     3121 aagaagaagc caatgatgta tggaaagcag cagagtttct acaaatgcta gaagctatca
     3181 aagcccttga agtcaggaac aaagaaaact cagctccctt agaggaaaat accacaggaa
     3241 aaaatgaggc caaaaaaagg aagattgcag aaacttcaaa tgttatcact gagtcattgc
     3301 catctgcaga atcagaacct gttgaaattg aggtagagat tgccgaaggc accattgaag
     3361 tggaagatga aggcatcgaa acattagagg aagtggcttc tgccaagcag tccgtaaagt
     3421 acatacagag cacaggttcc tctgatgatt ctgctctagc actgttggca gatattacca
     3481 gcaagtaccg tcaaggtgac agaaaagggc agattaaaga agatggctgt ccatctgacc
     3541 ccacgagcaa acaggtagaa ggtattgaaa ttgtggaact tcagctgtca catgtgaagg
     3601 acttgttcca ttgtgagaaa tgtaaccgtt catttaaatt gttttaccat tttaaggagc
     3661 acatgaaatc acactccact gagagtttca agtgtgaaat atgcaataaa cgatatcttc
     3721 gagagagcgc atggaaacag cacctaaatt gttaccacct tgaagaaggt ggagtcagta
     3781 agaagcaaag aactgggaaa aaaattcatg tatgtcagta ctgtgagaaa cagtttgacc
     3841 attttggaca ttttaaagaa catcttcgaa aacatacagg tgaaaaacct tttgaatgtc
     3901 caaattgtca tgaacgattt gctagaaata gcactctgaa atgtcacctc actgcatgcc
     3961 aaactggagt aggggcaaaa aaaggaagga agaagctcta cgaatgccag gtctgcaaca
     4021 gtgtgtttaa cagctgggac cagttcaaag atcacttggt aatacacact ggagataaac
     4081 ccaaccattg tactttatgt gatttgtggt ttatgcaagg aaatgaatta aggaggcatc
     4141 tcagtgatgc tcacaatatt tcagagcgtc tagtaacgga agaagttctt tcagtagaaa
     4201 cacgtgtgca aactgaacct gtaacatcaa tgactattat agaacaagtt gggaaggtgc
     4261 atgtgctacc attgcttcag gttcaggtgg attcagcaca agtgactgtg gaacaagtcc
     4321 atccagatct gctccaggac agccaggtgc acgattcaca catgagtgag cttccagagc
     4381 aggtccaagt gagttatcta gaagtgggcc gaattcagac tgaagaaggt actgaagtac
     4441 atgtagagga gctgcatgtt gaacgggtca atcaaatgcc agtggaagta caaactgaac
     4501 ttctagaagc agatttggac cacgtgaccc cagaaatcat gaaccaagag gagagagagt
     4561 ctagccaagc agatgctgct gaggctgcca gggaagatca cgaagatgct gaggatttag
     4621 agaccaagcc aacagtggat tctgaagcag aaaaggcaga gaatgaggac agaacagctc
     4681 tgccagtttt agaagagcaa aagctcattt ctgaagagga cttgtgagga tcccgcgact
     4741 ctagataatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
     4801 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
     4861 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
     4921 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
     4981 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
     5041 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
     5101 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
     5161 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
     5221 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatgac
     5281 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     5341 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     5401 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     5461 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     5521 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     5581 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     5641 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     5701 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     5761 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     5821 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaccgcg
     5881 tctggaacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     5941 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     6001 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     6061 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     6121 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     6181 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     6241 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     6301 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     6361 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     6421 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     6481 gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     6541 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     6601 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     6661 ctttttaaaa gaaaagaggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     6721 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
     6781 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     6841 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
     6901 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
     6961 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
     7021 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
     7081 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
     7141 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     7201 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     7261 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     7321 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     7381 gtgagaggcc ttgacattgc tagcgtttta ccgtcgacct ctagctagag cttggcgtaa
     7441 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7501 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7561 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7621 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7681 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7741 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7801 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7861 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7921 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7981 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     8041 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     8101 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     8161 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8221 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8281 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8341 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8401 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8461 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8521 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8581 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8641 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8701 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8761 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8821 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8881 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8941 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     9001 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9061 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     9121 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     9181 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     9241 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9301 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9361 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9421 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9481 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9541 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     9601 gtcgacggat cgggagatca acttgtttat tgcagcttat aatggttaca aataaagcaa
     9661 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     9721 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
     9781 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
     9841 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
     9901 atgtactaca aacttagtag tttttaaaga aattgtattt gttaaatatg tactacaaac
     9961 ttagtagt
//
