LOCUS V037904 9585 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V037904 VERSION V037904 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2827..4281 /label="ZDHHC1(NM_013304)" /note="ZDHHC1(NM_013304)" /gene="ZDHHC1" CDS 4288..4353 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 4373..4872 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 4893..5492 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5506..6094 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6301..6934 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(7063..7079) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7087..7103 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7111..7141) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7156..7177 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7465..8053) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8224..9084) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9085..9189) /gene="bla" /label="AmpR promoter" polyA_signal 9237..9371 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 gccaccatgt acaagatgaa catctgcaac aagccctcca acaagacggc ccctgagaag 2881 agtgtgtgga cggcaccggc acagcccagc ggaccctccc ctgagctgca gggccagcga 2941 tcccgccgga atgggtggag ctggccccct cacccgctcc agattgtggc ctggctgctg 3001 tacctcttct ttgctgtgat cggctttggg atccttgttc ccctcctgcc tcaccactgg 3061 gtgcccgctg gctacgcttg catgggcgcc atctttgctg gccaccttgt ggtgcacctg 3121 accgccgtct ccatcgatcc agcagatgcc aacgtgcggg acaagagcta tgcggggccc 3181 ctgcccatct tcaaccgaag ccagcacgca catgtcattg aagacctgca ctgcaacttg 3241 tgcaacgtgg atgtgagcgc tcgctccaag cactgcagcg cctgcaacaa gtgcgtgtgc 3301 ggtttcgacc accactgcaa gtggctcaac aactgtgtgg gcgagcggaa ctaccggctc 3361 tttctacaca gtgttgcatc cgctttactg ggcgtcctgc tcctggtgct ggtggccaca 3421 tatgtcttcg tggagttctt tgtcaacccc atgcgtctgc gcaccaaccg acactttgaa 3481 gtcctgaaga atcacacgga tgtgtggttc gtgttcctgc ctgccgcccc cgtggagacc 3541 caggcccctg ccatcctggc cctggccgcc ctgctcatcc ttctgggcct cctgtccaca 3601 gccctcctgg ggcacctgct ctgcttccac atttatctca tgtggcacaa gctcaccacc 3661 tatgagtaca tcgtgcagca ccgcccacca caggaggcca agggggttca cagggagctc 3721 gagtcatgtc ctcccaagat gcggcccatt caggagatgg agttctacat gcggaccttc 3781 agacatatgc gcccagagcc ccctggccag gccgggccag cagcagtgaa tgccaaacac 3841 tctcgccctg cctccccgga tccgacccca ggtaggaggg actgtgctgg gcctccggtc 3901 caggtggagt gggatagaaa gaagcctcta ccctggcgct cgcctctgct tcttttggcg 3961 atgtggggcc ctcaggctcc cccgtgtctc tgcagaaaaa gaggaagagg cgcgtgtata 4021 aagtgcgaac gtctgagacc tcggatccgg cgtcggggcc tagggccccc agccgccgct 4081 ccagctcgtc gacggattcc gcggacgcca gccctgtgca cgccgctggc cctgccggcg 4141 cctaccactc ggcgtcggca gagtccgtgg acgagattcc agtggcgcag acgcgcctgg 4201 gcagcgccgc tctggccgcc ccgcggggcc ggggccgaca gcccacgctg gcgcggcagg 4261 cgcgtgcgcc cgccgttttc gggatccgac tacaaagacc atgacggtga ttataaagat 4321 catgacatcg actacaagga tgacgatgac aagtagtcta gataattcta ccgggtaggg 4381 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg 4441 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg 4501 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc 4561 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact 4621 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc 4681 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc 4741 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc 4801 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct 4861 ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc acggtgcgcc 4921 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact 4981 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc 5041 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg 5101 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg 5161 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg 5221 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg 5281 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg 5341 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct 5401 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca 5461 cctggtgcat gacccgcaag cccggtgcct gaccgcgtct ggaacaatca acctctggat 5521 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 5581 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 5641 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 5701 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 5761 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 5821 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 5881 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc 5941 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 6001 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag 6061 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc 6121 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct 6181 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa 6241 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac 6301 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca 6361 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 6421 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 6481 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 6541 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 6601 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 6661 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 6721 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 6781 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 6841 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 6901 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt 6961 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattgctag 7021 cgttttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt 7081 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 7141 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 7201 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 7261 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 7321 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 7381 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 7441 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 7501 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 7561 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 7621 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 7681 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 7741 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 7801 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 7861 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat 7921 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 7981 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 8041 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 8101 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 8161 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 8221 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 8281 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 8341 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 8401 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 8461 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 8521 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 8581 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 8641 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 8701 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 8761 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 8821 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 8881 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 8941 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 9001 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 9061 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 9121 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 9181 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact 9241 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 9301 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 9361 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat 9421 accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg 9481 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttt 9541 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt //