LOCUS V046916 9715 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V046916 VERSION V046916 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..4471 /label="YES1(NM_005433)" /note="YES1(NM_005433)" /gene="YES1" promoter 4503..5002 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 5023..5622 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5636..6224 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6431..7064 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(7193..7209) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7217..7233 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7241..7271) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7286..7307 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7595..8183) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8354..9214) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9215..9319) /gene="bla" /label="AmpR promoter" polyA_signal 9367..9501 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatgggctg cattaaaagt aaagaaaaca aaagtccagc 2881 cattaaatac agacctgaaa atactccaga gcctgtcagt acaagtgtga gccattatgg 2941 agcagaaccc actacagtgt caccatgtcc gtcatcttca gcaaagggaa cagcagttaa 3001 tttcagcagt ctttccatga caccatttgg aggatcctca ggggtaacgc cttttggagg 3061 tgcatcttcc tcattttcag tggtgccaag ttcatatcct gctggtttaa caggtggtgt 3121 tactatattt gtggccttat atgattatga agctagaact acagaagacc tttcatttaa 3181 gaagggtgaa agatttcaaa taattaacaa tacggaagga gattggtggg aagcaagatc 3241 aatcgctaca ggaaagaatg gttatatccc gagcaattat gtagcgcctg cagattccat 3301 tcaggcagaa gaatggtatt ttggcaaaat ggggagaaaa gatgctgaaa gattactttt 3361 gaatcctgga aatcaacgag gtattttctt agtaagagag agtgaaacaa ctaaaggtgc 3421 ttattccctt tctattcgtg attgggatga gataaggggt gacaatgtga aacactacaa 3481 aattaggaaa cttgacaatg gtggatacta tatcacaacc agagcacaat ttgatactct 3541 gcagaaattg gtgaaacact acacagaaca tgctgatggt ttatgccaca agttgacaac 3601 tgtgtgtcca actgtgaaac ctcagactca aggtctagca aaagatgctt gggaaatccc 3661 tcgagaatct ttgcgactag aggttaaact aggacaagga tgtttcggcg aagtgtggat 3721 gggaacatgg aatggaacca cgaaagtagc aatcaaaaca ctaaaaccag gtacaatgat 3781 gccagaagct ttccttcaag aagctcagat aatgaaaaaa ttaagacatg ataaacttgt 3841 tccactatat gctgttgttt ctgaagaacc aatttacatt gtcactgaat ttatgtcaaa 3901 aggaagctta ttagatttcc ttaaggaagg agatggaaag tatttgaagc ttccacagct 3961 ggttgatatg gctgctcaga ttgctgatgg tatggcatat attgaaagaa tgaactatat 4021 tcaccgagat cttcgggctg ctaatattct tgtaggagaa aatcttgtgt gcaaaatagc 4081 agactttggt ttagcaaggt taattgaaga caatgaatac acagcaagac aaggtgcaaa 4141 atttccaatc aaatggacag ctcctgaagc tgcactgtat ggtcggttta caataaagtc 4201 tgatgtctgg tcatttggaa ttctgcaaac agaactagta acaaagggcc gagtgccata 4261 tccaggtatg gtgaaccgtg aagtattaga acaagtggag cgaggataca ggatgccgtg 4321 ccctcagggc tgtccagaat ccctccatga attgatgaat ctgtgttgga agaaggaccc 4381 tgatgaaaga ccaacatttg aatatattca gtccttcttg gaagactact tcactgctac 4441 agagccacag taccagccag gagaaaattt ataaggatcc cgcgactcta gataattcta 4501 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct 4561 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag 4621 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt 4681 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc 4741 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct 4801 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag 4861 gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc 4921 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc 4981 ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc 5041 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg 5101 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc 5161 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 5221 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 5281 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 5341 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 5401 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 5461 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 5521 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 5581 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaccgcgtct ggaacaatca 5641 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 5701 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 5761 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 5821 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 5881 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 5941 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 6001 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg 6061 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 6121 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 6181 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc 6241 agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct 6301 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag 6361 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa 6421 aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg 6481 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc 6541 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta 6601 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg 6661 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac 6721 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag 6781 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga 6841 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga 6901 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 6961 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 7021 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca 7081 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg 7141 acattgctag cgttttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 7201 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 7261 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 7321 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 7381 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 7441 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 7501 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 7561 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 7621 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 7681 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 7741 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 7801 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 7861 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 7921 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 7981 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 8041 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 8101 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 8161 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 8221 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 8281 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 8341 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 8401 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 8461 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 8521 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 8581 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 8641 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 8701 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 8761 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 8821 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 8881 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 8941 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 9001 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 9061 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 9121 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 9181 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 9241 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 9301 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg 9361 gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 9421 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 9481 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt 9541 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt 9601 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac 9661 ttagtagttt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt //