LOCUS V027748 9118 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V027748 VERSION V027748 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..3814 /label="YBX1(NM_004559)" /note="YBX1(NM_004559)" /gene="YBX1" CDS 3821..3886 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 3906..4405 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 4426..5025 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5039..5627 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5834..6467 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(6596..6612) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6620..6636 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6644..6674) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6689..6710 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6998..7586) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7757..8617) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8618..8722) /gene="bla" /label="AmpR promoter" polyA_signal 8770..8904 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatgagcag cgaggccgag acccagcagc cgcccgccgc 2881 cccccccgcc gcccccgccc tcagcgccgc cgacaccaag cccggcacta cgggcagcgg 2941 cgcagggagc ggtggcccgg gcggcctcac atcggcggcg cctgccggcg gggacaagaa 3001 ggtcatcgca acgaaggttt tgggaacagt aaaatggttc aatgtaagga acggatatgg 3061 tttcatcaac aggaatgaca ccaaggaaga tgtatttgta caccagactg ccataaagaa 3121 gaataacccc aggaagtacc ttcgcagtgt aggagatgga gagactgtgg agtttgatgt 3181 tgttgaagga gaaaagggtg cggaggcagc aaatgttaca ggtcctggtg gtgttccagt 3241 tcaaggcagt aaatatgcag cagaccgtaa ccattataga cgctatccac gtcgtagggg 3301 tcctccacgc aattaccagc aaaattacca gaatagtgag agtggggaaa agaacgaggg 3361 atcggagagt gctcccgaag gccaggccca acaacgccgg ccctaccgca ggcgaaggtt 3421 cccaccttac tacatgcgga gaccctatgg gcgtcgacca cagtattcca accctcctgt 3481 gcagggagaa gtgatggagg gtgctgacaa ccagggtgca ggagaacaag gtagaccagt 3541 gaggcagaat atgtatcggg gatatagacc acgattccgc aggggccctc ctcgccaaag 3601 acagcctaga gaggacggca atgaagaaga taaagaaaat caaggagatg agacccaagg 3661 tcagcagcca cctcaacgtc ggtaccgccg caacttcaat taccgacgca gacgcccaga 3721 aaaccctaaa ccacaagatg gcaaagagac aaaagcagcc gatccaccag ctgagaattc 3781 gtccgctccc gaggctgagc agggcggggc tgagggatcc gactacaaag accatgacgg 3841 tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagtagt ctagataatt 3901 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 3961 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 4021 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 4081 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 4141 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 4201 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg 4261 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag 4321 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc 4381 ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatgac cgagtacaag 4441 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc 4501 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg 4561 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg 4621 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg 4681 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg 4741 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg 4801 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc 4861 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc 4921 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc 4981 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaccgcg tctggaacaa 5041 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 5101 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 5161 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 5221 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 5281 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 5341 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 5401 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc 5461 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 5521 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg 5581 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc 5641 tgcagtcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat 5701 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct 5761 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa 5821 gaaaagaggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg 5881 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg 5941 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag 6001 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg 6061 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat 6121 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac 6181 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg 6241 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt 6301 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 6361 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 6421 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg 6481 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc 6541 ttgacattgc tagcgtttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat 6601 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 6661 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 6721 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 6781 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 6841 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 6901 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 6961 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 7021 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 7081 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 7141 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 7201 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 7261 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 7321 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 7381 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 7441 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 7501 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 7561 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 7621 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 7681 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 7741 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 7801 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 7861 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 7921 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 7981 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 8041 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 8101 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 8161 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 8221 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 8281 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 8341 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 8401 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 8461 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 8521 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 8581 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 8641 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 8701 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat 8761 cgggagatca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 8821 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 8881 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa 8941 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc 9001 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca 9061 aacttagtag tttttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt //