LOCUS       V019679                11223 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V019679
VERSION     V019679
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2830..2856
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4219..5979
                     /label="USP38(NM_001290326)"
                     /note="USP38(NM_001290326)"
                     /gene="USP38"
     promoter        6011..6510
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6531..7130
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7144..7732
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7939..8572
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8701..8717)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8725..8741
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8749..8779)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8794..8815
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9103..9691)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9862..10722)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10723..10827)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10875..11009
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 gccaccatgt acccatacga cgtaccagat tacgctgaca agatcctgga gggccttgtg
     2881 agttcctcgc atcccctgcc cctcaagcgg gtgattgtgc ggaaggtggt ggaatcggcg
     2941 gagcactggc tagacgaggc gcagtgcgag gccatgtttg acctgacgac ccggctcatc
     3001 ctggagggcc aggacccttt ccagcggcag gtggggcacc aggtgctgga ggcctacgca
     3061 cgataccacc ggccagagtt cgagtccttc ttcaacaaga ccttcgtgtt gggcctcctt
     3121 catcagggct accactctct ggacaggaag gatgtagcca tcctggacta cattcacaac
     3181 ggcctgaagc tgattatgag ctgtccgtcg gtgctggatc tctttagcct cctgcaggta
     3241 gaggtgttac ggatggtgtg tgagaggccg gagccgcagc tctgtgcccg actgagcgac
     3301 cttctgaccg actttgtgca atgcatcccc aaggggaaat tgtccatcac gttctgtcaa
     3361 cagctggttc gaacgatagg ccatttccag tgcgtgtcca cccaggaaag agagctgcgg
     3421 gaatatgtct cccaggtgac aaaagtgagt aacttgctgc agaacatctg gaaggccgag
     3481 cctgccacac tactgccttc cctgcaagaa gtttttgcaa gcatctcttc cacagatgca
     3541 tcatttgaac cttctgtagc attggcaagc cttgtgcagc atattcctct tcagatgatt
     3601 acagttctca tcaggagcct tactacggat ccaaatgtaa aagatgcaag tatgacccaa
     3661 gccctttgca gaatgattga ctggctatcc tggccattgg ctcagcatgt ggatacatgg
     3721 gtaattgcac tcctgaaagg actggcagct gtccagaagt ttactatttt gatagatgtt
     3781 actttgctga aaatagaact ggtttttaat cgactttggt ttcctcttgt gagacctggt
     3841 gctcttgcag ttctttctca catgctgctt agctttcagc attctccaga ggcgttccat
     3901 ttgattgttc ctcatgtggt taatttggtt cattctttca aaaatgatgg tctgccttca
     3961 agtacagcct tcttagtaca attaacagaa ttgatacact gtatgatgta tcattattct
     4021 ggatttccag atctctatga acctattctg gaggcaataa aggattttcc taagcccagt
     4081 gaagagaaga ttaagttaat tctcaatcaa agtgcctgga cttctcaatc caattctttg
     4141 gcgtcttgct tgtctagact ttctggaaaa tctgaaactg ggaaaactgg tcttattaac
     4201 ctaggaaata catgttatat gaacagtgtt atacaagcct tgtttatggc cacagatttc
     4261 aggagacaag tattatcttt aaatctaaat gggtgcaatt cattaatgaa aaaattacag
     4321 catctttttg cctttctggc ccatacacag agggaagcat acgcacctcg gatattcttt
     4381 gaggcttcca gacctccatg gtttactccc agatcacagc aagactgttc tgaatacctc
     4441 agatttctcc ttgacaggct ccatgaagaa gaaaagatct tgaaagttca ggcctcacac
     4501 aagccttctg aaattctgga atgcagtgaa acttctttac aggaagtagc tagtaaagca
     4561 gcagtactaa cagagacccc tcgtacaagt gacggtgaga agactttaat agaaaaaatg
     4621 tttggaggaa aactacgaac tcacatacgt tgtttgaact gcaggagtac ctcacaaaaa
     4681 gtggaagcct ttacagatct ttcgcttgcc ttttgtcctt cctcttcttt ggaaaacatg
     4741 tctgtccaag atccagcatc atcacccagt atacaagatg gtggtctaat gcaagcctct
     4801 gtacccggtc cttcagaaga accagtagtt tataatccaa caacagctgc cttcatctgt
     4861 gactcacttg tgaatgaaaa aaccataggc agtcctccta atgagtttta ctgttctgaa
     4921 aacacttctg tccctaacga atctaacaag attcttgtta ataaagatgt acctcagaaa
     4981 ccaggaggtg aaaccacacc ttcagtaact gacttactaa attatttttt ggctccagag
     5041 attcttactg gtgataacca atattattgt gaaaactgtg cctctctgca aaatgctgag
     5101 aaaactatgc aaatcacgga ggaacctgaa taccttattc ttactctcct gagattttca
     5161 tatgatcaga agtatcatgt gagaaggaaa attttagaca atgtatcact gccactggtt
     5221 ttggagttgc cagttaaaag aattacttct ttctcttcat tgtcagaaag ttggtctgta
     5281 gatgttgact tcactgatct tagtgagaac cttgctaaaa aattaaagcc ttcagggact
     5341 gatgaagctt cctgcacaaa attggtgccc tatctattaa gttccgttgt ggttcactct
     5401 ggtatatcct ctgaaagtgg gcattactat tcttatgcca ggaatatcac aagtacagac
     5461 tcttcatatc agatgtacca ccagtctgag gctctggcat tagcatcctc ccagagtcat
     5521 ttactaggga gagatagtcc cagtgcagtt tttgaacagg atttggaaaa taaggaaatg
     5581 tcaaaagaat ggtttttatt taatgacagt agagtgacat ttacttcatt tcagtcagtc
     5641 cagaaaatta cgagcaggtt tccaaaggac acagcttatg tgcttttgta taaaaaacag
     5701 catagtacta atggtttaag tggtaataac ccaaccagtg gactctggat aaatggagac
     5761 ccacctctac agaaagaact tatggatgct ataacaaaag acaataaact atatttacag
     5821 gaacaagagt tgaatgctcg agcccgggcc ctccaagctg catctgcttc atgttcattt
     5881 cggcccaatg gatttgatga caacgaccca ccaggaagct gtggaccaac tggtggaggg
     5941 ggtggaggag gatttaatac agttggcaga ctcgtatttt gaggatcccg cgactctaga
     6001 taattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
     6061 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
     6121 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
     6181 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
     6241 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
     6301 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
     6361 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
     6421 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
     6481 ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt
     6541 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     6601 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     6661 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     6721 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     6781 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     6841 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     6901 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     6961 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     7021 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     7081 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga ccgcgtctgg
     7141 aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     7201 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     7261 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     7321 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     7381 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     7441 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     7501 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     7561 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     7621 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     7681 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt
     7741 aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     7801 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     7861 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     7921 taaaagaaaa gaggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     7981 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
     8041 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
     8101 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
     8161 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
     8221 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
     8281 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
     8341 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
     8401 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     8461 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     8521 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     8581 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     8641 aggccttgac attgctagcg ttttaccgtc gacctctagc tagagcttgg cgtaatcatg
     8701 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     8761 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     8821 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     8881 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     8941 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9001 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9061 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9121 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9181 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9241 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9301 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9361 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9421 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9481 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9541 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9601 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9661 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9721 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9781 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9841 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9901 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9961 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10021 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10081 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10141 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    10201 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10261 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10321 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10381 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10441 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10501 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10561 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10621 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10681 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    10741 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    10801 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
    10861 cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
    10921 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    10981 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
    11041 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
    11101 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
    11161 ctacaaactt agtagttttt aaagaaattg tatttgttaa atatgtacta caaacttagt
    11221 agt
//
