LOCUS       V032956                12091 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032956
VERSION     V032956
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..6091
                     /label="UBE3C(NM_014671)"
                     /note="UBE3C(NM_014671)"
                     /gene="UBE3C"
     CDS             6143..6862
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     promoter        6879..7378
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             7399..7998
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8012..8600
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8807..9440
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(9569..9585)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9593..9609
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9617..9647)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9662..9683
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9971..10559)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10730..11590)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11591..11695)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11743..11877
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatgttcag cttcgaaggc gacttcaaga cgcggcccaa
     2881 ggtgtccctt ggcggcgcga gcaggaagga ggaaaaggct tctcttttac atcgtactca
     2941 ggaagaaaga agaaagagag aggaagaaag gcgaaggttg aaaaatgcaa taattatcca
     3001 gtcatttatt cgaggctata gagacagaaa acagcaatat tccatccaaa gaagtgcatt
     3061 tgatcgctgt gctaccttgt cacagtccgg gggcgctttt cccattgcta atggccccaa
     3121 ccttaccctt ttggtaaggc agcttctgtt tttttacaaa caaaatgaag actcaaaacg
     3181 tttgatatgg ctgtatcaga acttaattaa acacagctct ctgtttgtca agcagttgga
     3241 tggatctgag agacttacat gcttatttca gataaaaaga ttgatgagcc tctgttgcag
     3301 gttgctgcaa aactgtaatg atgacagttt gaatgttgca cttccaatga gaatgcttga
     3361 agtattttcg tctgagaata cttacttgcc tgttttacaa gatgctagct atgtggtgtc
     3421 agtgattgaa caaattttgc actacatgat tcacaatggg tattataggt ctctatattt
     3481 gttgattaac agcaagcttc catcaagtat tgaatattct gatttatctc gagttcctat
     3541 agcaaaaatt ttgctagaga atgttctaaa accattgcac tttacttaca actcctgtcc
     3601 ggaaggtgcg aggcaacaag tttttacagc cttcacagag gagtttctgg cagcaccttt
     3661 tacagatcag atttttcatt tcatcattcc ggcgcttgca gatgcgcaga ccgttttccc
     3721 ttacgagccc tttctgaatg cactgttgtt aatagagagt agatgttcaa gaaagagtgg
     3781 tggagcaccc tggcttttct atttcgtttt aactgttggc gaaaattatt tgggggccct
     3841 ctctgaggaa gggctgctgg tgtatttgcg ggtgctgcag accttcctct ctcagttacc
     3901 agtctctcct gccagcgcga gctgtcacga ctcagccagt gactctgagg aggagagtga
     3961 agaagccgac aagccctcaa gcccggagga tggcagactg tcagtatcat acataacaga
     4021 ggaatgcctg aagaagctgg acacaaagca gcagaccaac accctgctca acctggtgtg
     4081 gagggactct gcgagcgagg aggtcttcac caccatggcc tccgtctgcc acacgctgat
     4141 ggtgcagcac cgcatgatgg tacccaaagt caggcttctc tacagtttag cctttaatgc
     4201 caggtttctg agacatcttt ggtttctaat atcttccatg tcaacacgga tgatcacagg
     4261 gtctatggta ccgttgcttc aggtgatatc caggggttct cctatgtctt ttgaagattc
     4321 tagtcgaatc atcccactct tttatctttt tagctccttg tttagtcatt cactaatttc
     4381 catacatgat aacgaattct tcggtgatcc catagaagtt gtaggtcaaa gacaatcatc
     4441 aatgatgcct tttactttag aagagctgat aatgttgtct cgatgccttc gagatgcatg
     4501 cctggggatc atcaagttgg cttatccaga aaccaagcca gaagttcgag aagaatatat
     4561 tacagcattt cagagtattg gagttactac tagctctgaa atgcaacaat gcatacagat
     4621 ggaacagaaa agatggattc agttatttaa ggttatcacc aatctagtga aaatgttgaa
     4681 gtccagagac acgaggagaa atttttgtcc tccaaaccac tggctgtcag aacaagaaga
     4741 tattaaagca gataaggtca ctcagctcta tgtgccagca tccagacatg tgtggaggtt
     4801 ccggcggatg gggaggatag gcccgctgca gtccaccctg gacgtgggtt tggagtcccc
     4861 gccgctgtct gtgtctgagg aaagacagct tgctgtcctg acagagttgc cttttgtggt
     4921 tccatttgag gaacgagtaa agatctttca gaggttgatt tatgcagata agcaagaagt
     4981 tcaaggagat ggtccatttc tggatggaat taatgtcaca ataagaagaa attacattta
     5041 tgaagatgct tatgacaaac tttctccaga aaatgagcct gatttgaaaa agcggatccg
     5101 tgtgcacttg ctcaatgccc atggcctgga tgaagctggc attgatggtg gtggtatttt
     5161 cagagagttt ttaaatgaac tactgaagtc aggatttaac cccaaccagg ggttctttaa
     5221 gactactaat gaagggcttc tgtaccccaa cccggctgct cagatgcttg tgggagattc
     5281 ttttgccaga cattactact tcctaggcag aatgcttgga aaggctctct atgagaacat
     5341 gctggtggag ctgccctttg caggcttctt tctttccaag ttgcttggaa ccagtgccga
     5401 cgtggacatt caccacctcg cctccctaga ccctgaggtg tataagaatt tgctctttct
     5461 gaagagctac gaagacgatg tggaggagct tgggctgaac ttcactgtgg tgaacaatga
     5521 cctgggagag gcgcaggtag ttgaactaaa attcggtggg aaagacatcc ctgtcaccag
     5581 cgccaaccgg attgcgtaca tccacttggt ggcagactac aggctgaaca ggcagatccg
     5641 ccagcactgc ctggctttcc gccagggcct tgccaatgtc gtcagcctcg agtggctccg
     5701 aatgtttgat cagcaagaaa ttcaggtatt aatttctggt gcacaagttc ccataagcct
     5761 agaggaccta aaatccttta caaactattc aggaggctat tctgcagacc atcctgttat
     5821 taaggtcttc tggagagttg tggaagggtt cactgatgaa gaaaagcgca aactgctgaa
     5881 gtttgtaaca agctgctctc gaccccctct cttggggttt aaggagttgt atcccgcatt
     5941 ttgtattcac aacggaggct ccgaccttga gcggctcccc acagccagca cctgcatgaa
     6001 cctgctgaag ctccccgagt tctatgacga gacacttttg cgaagtaaac ttctctatgc
     6061 gattgaatgt gccgctggct ttgagctgag cggcggcgga gggagtggag gagggggaag
     6121 cggaggcgga ggatccgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     6181 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     6241 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     6301 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     6361 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     6421 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     6481 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     6541 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     6601 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     6661 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     6721 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     6781 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     6841 catggacgag ctgtacaagt agtctagata attctaccgg gtaggggagg cgcttttccc
     6901 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
     6961 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
     7021 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
     7081 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
     7141 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
     7201 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
     7261 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
     7321 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac
     7381 ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     7441 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     7501 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     7561 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     7621 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     7681 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     7741 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     7801 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     7861 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     7921 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     7981 cgcaagcccg gtgcctgacc gcgtctggaa caatcaacct ctggattaca aaatttgtga
     8041 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     8101 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     8161 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     8221 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     8281 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     8341 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     8401 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     8461 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     8521 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     8581 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga
     8641 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     8701 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     8761 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt
     8821 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     8881 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     8941 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     9001 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     9061 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     9121 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     9181 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     9241 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     9301 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9361 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9421 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     9481 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt ttaccgtcga
     9541 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     9601 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     9661 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     9721 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     9781 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     9841 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     9901 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     9961 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    10021 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    10081 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    10141 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    10201 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    10261 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    10321 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    10381 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
    10441 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    10501 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    10561 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    10621 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
    10681 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    10741 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    10801 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    10861 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    10921 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    10981 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    11041 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    11101 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    11161 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    11221 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    11281 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    11341 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    11401 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    11461 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    11521 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    11581 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    11641 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    11701 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct
    11761 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    11821 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
    11881 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
    11941 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
    12001 taaagaaatt gtatttgtta aatatgtact acaaacttag tagtttttaa agaaattgta
    12061 tttgttaaat atgtactaca aacttagtag t
//
