LOCUS       V044945                11143 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044945
VERSION     V044945
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..5194
                     /label="TLR2(NM_001318787)"
                     /note="TLR2(NM_001318787)"
                     /gene="TLR2"
     CDS             5231..5950
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="Aequorea coerulescens GFP"
                     /transl_table=1
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK*"
                     /label="AcGFP1"
     promoter        5978..6477
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6498..7097
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7111..7699
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7906..8539
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8668..8684)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8692..8708
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8716..8746)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8761..8782
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9070..9658)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9829..10689)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10690..10794)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10842..10976
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatgccaca tactttgtgg atggtgtggg tcttgggggt
     2881 catcatcagc ctctccaagg aagaatcctc caatcaggct tctctgtctt gtgaccgcaa
     2941 tggtatctgc aagggcagct caggatcttt aaactccatt ccctcagggc tcacagaagc
     3001 tgtaaaaagc cttgacctgt ccaacaacag gatcacctac attagcaaca gtgacctaca
     3061 gaggtgtgtg aacctccagg ctctggtgct gacatccaat ggaattaaca caatagagga
     3121 agattctttt tcttccctgg gcagtcttga acatttagac ttatcctata attacttatc
     3181 taatttatcg tcttcctggt tcaagcccct ttcttcttta acattcttaa acttactggg
     3241 aaatccttac aaaaccctag gggaaacatc tcttttttct catctcacaa aattgcaaat
     3301 cctgagagtg ggaaatatgg acaccttcac taagattcaa agaaaagatt ttgctggact
     3361 taccttcctt gaggaacttg agattgatgc ttcagatcta cagagctatg agccaaaaag
     3421 tttgaagtca attcagaatg taagtcatct gatccttcat atgaagcagc atattttact
     3481 gctggagatt tttgtagatg ttacaagttc cgtggaatgt ttggaactgc gagatactga
     3541 tttggacact ttccattttt cagaactatc cactggtgaa acaaattcat tgattaaaaa
     3601 gtttacattt agaaatgtga aaatcaccga tgaaagtttg tttcaggtta tgaaactttt
     3661 gaatcagatt tctggattgt tagaattaga gtttgatgac tgtaccctta atggagttgg
     3721 taattttaga gcatctgata atgacagagt tatagatcca ggtaaagtgg aaacgttaac
     3781 aatccggagg ctgcatattc caaggtttta cttattttat gatctgagca ctttatattc
     3841 acttacagaa agagttaaaa gaatcacagt agaaaacagt aaagtttttc tggttccttg
     3901 tttactttca caacatttaa aatcattaga atacttggat ctcagtgaaa atttgatggt
     3961 tgaagaatac ttgaaaaatt cagcctgtga ggatgcctgg ccctctctac aaactttaat
     4021 tttaaggcaa aatcatttgg catcattgga aaaaaccgga gagactttgc tcactctgaa
     4081 aaacttgact aacattgata tcagtaagaa tagttttcat tctatgcctg aaacttgtca
     4141 gtggccagaa aagatgaaat atttgaactt atccagcaca cgaatacaca gtgtaacagg
     4201 ctgcattccc aagacactgg aaattttaga tgttagcaac aacaatctca atttattttc
     4261 tttgaatttg ccgcaactca aagaacttta tatttccaga aataagttga tgactctacc
     4321 agatgcctcc ctcttaccca tgttactagt attgaaaatc agtaggaatg caataactac
     4381 gttttctaag gagcaacttg actcatttca cacactgaag actttggaag ctggtggcaa
     4441 taacttcatt tgctcctgtg aattcctctc cttcactcag gagcagcaag cactggccaa
     4501 agtcttgatt gattggccag caaattacct gtgtgactct ccatcccatg tgcgtggcca
     4561 gcaggttcag gatgtccgcc tctcggtgtc ggaatgtcac aggacagcac tggtgtctgg
     4621 catgtgctgt gctctgttcc tgctgatcct gctcacgggg gtcctgtgcc accgtttcca
     4681 tggcctgtgg tatatgaaaa tgatgtgggc ctggctccag gccaaaagga agcccaggaa
     4741 agctcccagc aggaacatct gctatgatgc atttgtttct tacagtgagc gggatgccta
     4801 ctgggtggag aaccttatgg tccaggagct ggagaacttc aatcccccct tcaagttgtg
     4861 tcttcataag cgggacttca ttcctggcaa gtggatcatt gacaatatca ttgactccat
     4921 tgaaaagagc cacaaaactg tctttgtgct ttctgaaaac tttgtgaaga gtgagtggtg
     4981 caagtatgaa ctggacttct cccatttccg tctttttgat gagaacaatg atgctgccat
     5041 tctcattctt ctggagccca ttgagaaaaa agccattccc cagcgcttct gcaagctgcg
     5101 gaagataatg aacaccaaga cctacctgga gtggcccatg gacgaggctc agcgggaagg
     5161 attttgggta aatctgagag ctgcgataaa gtccggatcc ggtggaggcg gatcaggagg
     5221 cggtggatct atggtgagca agggcgccga gctgttcacc ggcatcgtgc ccatcctgat
     5281 cgagctgaat ggcgatgtga atggccacaa gttcagcgtg agcggcgagg gcgagggcga
     5341 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcctgtgcc
     5401 ctggcccacc ctggtgacca ccctgagcta cggcgtgcag tgcttctcac gctaccccga
     5461 tcacatgaag cagcacgact tcttcaagag cgccatgcct gagggctaca tccaggagcg
     5521 caccatcttc ttcgaggatg acggcaacta caagtcgcgc gccgaggtga agttcgaggg
     5581 cgataccctg gtgaatcgca tcgagctgac cggcaccgat ttcaaggagg atggcaacat
     5641 cctgggcaat aagatggagt acaactacaa cgcccacaat gtgtacatca tgaccgacaa
     5701 ggccaagaat ggcatcaagg tgaacttcaa gatccgccac aacatcgagg atggcagcgt
     5761 gcagctggcc gaccactacc agcagaatac ccccatcggc gatggccctg tgctgctgcc
     5821 cgataaccac tacctgtcca cccagagcgc cctgtccaag gaccccaacg agaagcgcga
     5881 tcacatgatc tacttcggct tcgtgaccgc cgccgccatc acccacggca tggatgagct
     5941 gtacaagtga gcggccgcga ctctagataa ttctaccggg taggggaggc gcttttccca
     6001 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
     6061 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
     6121 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag
     6181 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg
     6241 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt
     6301 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg
     6361 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
     6421 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
     6481 tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     6541 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     6601 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     6661 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     6721 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     6781 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     6841 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     6901 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     6961 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     7021 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     7081 gcaagcccgg tgcctgaccg cgtctggaac aatcaacctc tggattacaa aatttgtgaa
     7141 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     7201 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     7261 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     7321 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     7381 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     7441 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     7501 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     7561 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     7621 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     7681 ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag
     7741 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
     7801 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca
     7861 aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc
     7921 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     7981 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
     8041 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
     8101 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
     8161 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
     8221 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
     8281 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
     8341 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     8401 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     8461 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     8521 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     8581 gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt taccgtcgac
     8641 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     8701 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8761 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8821 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8881 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8941 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     9001 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9061 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9121 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9181 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9241 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9301 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9361 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9421 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9481 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     9541 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9601 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9661 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9721 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9781 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9841 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9901 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9961 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    10021 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    10081 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    10141 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10201 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10261 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    10321 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    10381 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    10441 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10501 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10561 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10621 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10681 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10741 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10801 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt
    10861 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    10921 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
    10981 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
    11041 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    11101 aaagaaattg tatttgttaa atatgtacta caaacttagt agt
//
