LOCUS       V043794                11667 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043794
VERSION     V043794
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2827..6399
                     /label="Smc2(NM_001301412)"
                     /note="Smc2(NM_001301412)"
                     /gene="Smc2"
     CDS             6406..6435
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHHHHHH"
                     /label="10xHis"
     promoter        6455..6954
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6975..7574
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7588..8176
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8383..9016
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(9145..9161)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9169..9185
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9193..9223)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9238..9259
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9547..10135)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10306..11166)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11167..11271)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11319..11453
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 gccaccatgt atgttaaatc aatcattctc gaaggattca agtcctatgc acagaggact
     2881 gaagtcaatg gttttgaccc cctcttcaat gcgatcactg gtttaaatgg tagtggaaaa
     2941 tccaacatat tggactccat atgctttctc ctaggcatct ccaacctgtc ccaggtgcgg
     3001 gcttcaaatt tacaagatct cgtttataaa aatggacagg ctgggattac caaagcctct
     3061 gtttcaatca cttttgataa ttctgacaaa aagcagagtc cgttgggatt tgaagctcat
     3121 gatgaaatca cagtaacaag acaggtggtt attggtggca gaaataaata cttaatcaat
     3181 ggagtgaatg ccaacaacac cagagtccaa gatctctttt gttctgtggg cctgaatgtt
     3241 aacaaccctc actttctcat catgcagggc agaattacga aagtattaaa tatgaaacca
     3301 ccagagatat tatccatgat tgaagaagct gctggaacca ggatgtatga gtacaaaaaa
     3361 atagccgccc agaaaactat agaaaaaaag gaggctaagc tgaaagaaat aaaaacgata
     3421 ctggaagaag agattactcc aacgattcaa aaattaaaag aggaaagatc ttcttacttg
     3481 gagtaccaga aagtaatgcg ggagatagag catctgagtc gtttatacat tgcctatcag
     3541 tttttgcggg ctgaagacac caaggaacgc tcagctgggg agctaaagga aatgcaggat
     3601 aaaatagtaa atcttcaaga agtactgtca gagaatgaaa aaaaaataaa ggctctcaat
     3661 tgtgaaatcg aagaattgga gagaagaaaa gataaggaaa ctggaggcaa actcaagtct
     3721 ctagaggatg cttgtgcaga agcccagcga gttaatacta agtctcaaag tgcatttgac
     3781 ctcaagaaga aaaacctagc tagtgaagaa accaagcgca aagagctaca aaacagtatg
     3841 gctgaggatt ctaaagcatt agcagcaaag gaaaaagaag ttaagaagat aacagatgga
     3901 ctgcatggcc ttcaagaagc aagcaataaa gatgctgaag ctttggctgc tgcacagcag
     3961 cacttcaatg ctgtttctgc tggcttgtcc agtaacgaag atggagcaga agcaactctg
     4021 gctgggcaga tgatagcctg taaaaacgat atcagcaaag ctcagactga ggccaagcag
     4081 gctcagatga agctgaaaca tgcccaacaa gaactaaaga gtaagcaagc agaggttaag
     4141 aagatggaca gtggctacaa gaaggatcaa gacgcttttg aagctgtgaa aaaagctaaa
     4201 gaaaaacttg aaactgaaat gaagaagcta aattacgaag aaaacaaaga ggaaaagctt
     4261 ttggaaaagc acaggcagct gtcccgtgac atcaacaacc taaaaggaaa acatgaagct
     4321 ctcttggcca agtttcccaa tcttcaattt gcatacaagg acccagagaa gaactggaat
     4381 cgaaattctg tgaaaggact tgtagcttct ctgataaatg tgaaagataa ttctacagcc
     4441 acagctctgg aagtggtggc tggggaacga ctctacaatg ttgtagtaga cacagaggta
     4501 actgctaaga agctactaga aaagggggag ttgaagcgtc gatacacaat aattcctctc
     4561 aataagattt cagccagatg tattgctcct gaaactctga gggtcgctca gaatcttgtt
     4621 ggtcctgaca atgttcatgt agctctttcc ttggttgact ataaaccaga acttcagaaa
     4681 ggaatggaat ttgtctttgg aacaacattt gtttgtaaca atatggataa tgccaaaaaa
     4741 gtggcctttg ataaaaggat aatgaccaga actgtaaccc ttgggggtga tgtatttgat
     4801 cctcatggaa cattgagtgg aggtgctagg tcccaggcag cttcgatttt aaccaagttt
     4861 caagaagtca aagatgttca agatgagctg aggaccaagg agaatgaact gcgcgctcta
     4921 gaagaggagt tagcaggtct taaaaatgtt gctgaaaagt atcgccaact gaaacagcag
     4981 tgggagatga agacagagga gggagactta ttgcaaacta aacttcagca aagctcttat
     5041 cataagcaac aagaagaatt agatgccctt aaaaaaacca ttgaggaaag tgaggagaca
     5101 ttaaaaagca caaaagaaat ccaaaagaaa gctgaagaaa agtatgaagc attggagaat
     5161 aaaatgaaaa atgcggaagc tgaaagagag aaagaactga aggatgctca gaaaaaacta
     5221 gattgtgcca aaacaaaggc agatgcatct agcaagaaaa tgaaagaaaa acaacaggag
     5281 gttgaagcta ttactctaga gctggaggag ctcaaaagag agcatgcttc taatgagcag
     5341 cagcttgatg ccgtgaatga agccatcaaa gcctatgaag gtcagattga aaaaatggca
     5401 gctgaggtcg ctaaaaataa ggaatcagta aataaagctc aagacgagct gatgaagcaa
     5461 aagcaaataa taacagccca ggacaacata attaaagata aatgtgcaga agtggcgaaa
     5521 cacaatttgc aaaacaatga gtctcaactt aaaattaagg agttggacca cagcataagc
     5581 aaacataagc gggaagcgga tgatgctgct gcaaaggtat ccaaaatgtt aagtgattat
     5641 gattggatta atgctgagaa acacctattt ggccaaccta atagtgccta tgatttcaaa
     5701 acaaacaacc ccaaagaagc tggccagaga cttcagaagt tacaggaagt aaaagagaaa
     5761 ctagggagaa atgtcaacct gagagctatg aacgtgctga cagaagctga agagcggtat
     5821 aatgatttga tgaagaagaa aagaattgta gaaaatgaca aatccaaaat ccttgcaact
     5881 atagaagatc ttgaccagaa gaaaaaccag gccctaaata ttgcttggca aaaggtgaac
     5941 aaagactttg gctctatttt ttctaccctt ttgcctggtg ccaatgctat gcttgcacca
     6001 ccagaagggc agacagtttt agatggtctg gagttcaagg ttgccttagg aaatacgtgg
     6061 aaagaaaacc taactgagct tagtggtggt cagaggtctc tagtggctct ctcactaata
     6121 ttgtccatgc tcctcttcaa acctgctcca atttatatct tggatgaggt ggatgcggcc
     6181 ttggatcttt ctcataccca gaatattggg cagatgctgc ggactcactt cacacattct
     6241 caattcattg tggtatccct caaagaaggt atgttcaaca atgcaaacgt tctcttcaaa
     6301 accaagtttg tggatggtgt ttctacagta gccagattta cgcagagtca agctgggaag
     6361 attccgaagg aagcaaagtc cagaggaaaa gaacccaacg aattccatca tcatcatcat
     6421 catcatcatc atcattaatc tagataattc taccgggtag gggaggcgct tttcccaagg
     6481 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
     6541 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
     6601 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
     6661 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
     6721 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
     6781 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
     6841 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
     6901 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
     6961 agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     7021 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     7081 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     7141 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     7201 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     7261 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     7321 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     7381 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     7441 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     7501 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     7561 agcccggtgc ctgaccgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga
     7621 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     7681 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     7741 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7801 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7861 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     7921 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7981 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     8041 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     8101 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     8161 tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa
     8221 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     8281 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
     8341 cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact
     8401 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     8461 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
     8521 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
     8581 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
     8641 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
     8701 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
     8761 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     8821 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     8881 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     8941 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     9001 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     9061 aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgttttac cgtcgacctc
     9121 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     9181 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     9241 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     9301 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     9361 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     9421 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     9481 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     9541 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     9601 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     9661 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     9721 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9781 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9841 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9901 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9961 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
    10021 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    10081 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    10141 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    10201 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    10261 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    10321 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    10381 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    10441 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    10501 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    10561 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    10621 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    10681 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    10741 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10801 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10861 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10921 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10981 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    11041 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    11101 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    11161 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    11221 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    11281 ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata
    11341 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    11401 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
    11461 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
    11521 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
    11581 gaaattgtat ttgttaaata tgtactacaa acttagtagt ttttaaagaa attgtatttg
    11641 ttaaatatgt actacaaact tagtagt
//
