LOCUS       V019987                11630 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V019987
VERSION     V019987
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2826..6356
                     /label="SREBF1(NM_001005291)"
                     /note="SREBF1(NM_001005291)"
                     /gene="SREBF1"
     CDS             6363..6386
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     promoter        6418..6917
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6938..7537
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7551..8139
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8346..8979
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(9108..9124)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9132..9148
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9156..9186)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9201..9222
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9510..10098)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10269..11129)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11130..11234)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11282..11416
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ccaccatgga cgagccaccc ttcagcgagg cggctttgga gcaggcgctg ggcgagccgt
     2881 gcgatctgga cgcggcgctg ctgaccgaca tcgaaggtga agtcggcgcg gggaggggta
     2941 gggccaacgg cctggacgcc ccaagggcgg gcgcagatcg cggagccatg gattgcactt
     3001 tcgaagacat gcttcagctt atcaacaacc aagacagtga cttccctggc ctatttgacc
     3061 caccctatgc tgggagtggg gcagggggca cagaccctgc cagccccgat accagctccc
     3121 caggcagctt gtctccacct cctgccacat tgagctcctc tcttgaagcc ttcctgagcg
     3181 ggccgcaggc agcgccctca cccctgtccc ctccccagcc tgcacccact ccattgaaga
     3241 tgtacccgtc catgcccgct ttctcccctg ggcctggtat caaggaagag tcagtgccac
     3301 tgagcatcct gcagaccccc accccacagc ccctgccagg ggccctcctg ccacagagct
     3361 tcccagcccc agccccaccg cagttcagct ccacccctgt gttaggctac cccagccctc
     3421 cgggaggctt ctctacagga agccctcccg ggaacaccca gcagccgctg cctggcctgc
     3481 cactggcttc cccgccaggg gtcccgcccg tctccttgca cacccaggtc cagagtgtgg
     3541 tcccccagca gctactgaca gtcacagctg cccccacggc agcccctgta acgaccactg
     3601 tgacctcgca gatccagcag gtcccggtcc tgctgcagcc ccacttcatc aaggcagact
     3661 cgctgcttct gacagccatg aagacagacg gagccactgt gaaggcggca ggtctcagtc
     3721 ccctggtctc tggcaccact gtgcagacag ggcctttgcc gaccctggtg agtggcggaa
     3781 ccatcttggc aacagtccca ctggtcgtag atgcggagaa gctgcctatc aaccggctcg
     3841 cagctggcag caaggccccg gcctctgccc agagccgtgg agagaagcgc acagcccaca
     3901 acgccattga gaagcgctac cgctcctcca tcaatgacaa aatcattgag ctcaaggatc
     3961 tggtggtggg cactgaggca aagctgaata aatctgctgt cttgcgcaag gccatcgact
     4021 acattcgctt tctgcaacac agcaaccaga aactcaagca ggagaaccta agtctgcgca
     4081 ctgctgtcca caaaagcaaa tctctgaagg atctggtgtc ggcctgtggc agtggaggga
     4141 acacagacgt gctcatggag ggcgtgaaga ctgaggtgga ggacacactg accccacccc
     4201 cctcggatgc tggctcacct ttccagagca gccccttgtc ccttggcagc aggggcagtg
     4261 gcagcggtgg cagtggcagt gactcggagc ctgacagccc agtctttgag gacagcaagg
     4321 caaagccaga gcagcggccg tctctgcaca gccggggcat gctggaccgc tcccgcctgg
     4381 ccctgtgcac gctcgtcttc ctctgcctgt cctgcaaccc cttggcctcc ttgctggggg
     4441 cccgggggct tcccagcccc tcagatacca ccagcgtcta ccatagccct gggcgcaacg
     4501 tgctgggcac cgagagcaga gatggccctg gctgggccca gtggctgctg cccccagtgg
     4561 tctggctgct caatgggctg ttggtgctcg tctccttggt gcttctcttt gtctacggtg
     4621 agccagtcac acggccccac tcaggccccg ccgtgtactt ctggaggcat cgcaagcagg
     4681 ctgacctgga cctggcccgg ggagactttg cccaggctgc ccagcagctg tggctggccc
     4741 tgcgggcact gggccggccc ctgcccacct cccacctgga cctggcttgt agcctcctct
     4801 ggaacctcat ccgtcacctg ctgcagcgtc tctgggtggg ccgctggctg gcaggccggg
     4861 cagggggcct gcagcaggac tgtgctctgc gagtggatgc tagcgccagc gcccgagacg
     4921 cagccctggt ctaccataag ctgcaccagc tgcacaccat ggggaagcac acaggcgggc
     4981 acctcactgc caccaacctg gcgctgagtg ccctgaacct ggcagagtgt gcaggggatg
     5041 ccgtgtctgt ggcgacgctg gccgagatct atgtggcggc tgcattgaga gtgaagacca
     5101 gtctcccacg ggccttgcat tttctgacac gcttcttcct gagcagtgcc cgccaggcct
     5161 gcctggcaca gagtggctca gtgcctcctg ccatgcagtg gctctgccac cccgtgggcc
     5221 accgtttctt cgtggatggg gactggtccg tgctcagtac cccatgggag agcctgtaca
     5281 gcttggccgg gaacccagtg gaccccctgg cccaggtgac tcagctattc cgggaacatc
     5341 tcttagagcg agcactgaac tgtgtgaccc agcccaaccc cagccctggg tcagctgatg
     5401 gggacaagga attctcggat gccctcgggt acctgcagct gctgaacagc tgttctgatg
     5461 ctgcgggggc tcctgcctac agcttctcca tcagttccag catggccacc accaccggcg
     5521 tagacccggt ggccaagtgg tgggcctctc tgacagctgt ggtgatccac tggctgcggc
     5581 gggatgagga ggcggctgag cggctgtgcc cgctggtgga gcacctgccc cgggtgctgc
     5641 aggagtctga gagacccctg cccagggcag ctctgcactc cttcaaggct gcccgggccc
     5701 tgctgggctg tgccaaggca gagtctggtc cagccagcct gaccatctgt gagaaggcca
     5761 gtgggtacct gcaggacagc ctggctacca caccagccag cagctccatt gacaaggccg
     5821 tgcagctgtt cctgtgtgac ctgcttcttg tggtgcgcac cagcctgtgg cggcagcagc
     5881 agcccccggc cccggcccca gcagcccagg gcaccagcag caggccccag gcttccgccc
     5941 ttgagctgcg tggcttccaa cgggacctga gcagcctgag gcggctggca cagagcttcc
     6001 ggcccgccat gcggagggtg ttcctacatg aggccacggc ccggctgatg gcgggggcca
     6061 gccccacacg gacacaccag ctcctcgacc gcagtctgag gcggcgggca ggccccggtg
     6121 gcaaaggagg cgcggtggcg gagctggagc cgcggcccac gcggcgggag cacgcggagg
     6181 ccttgctgct ggcctcctgc tacctgcccc ccggcttcct gtcggcgccc gggcagcgcg
     6241 tgggcatgct ggctgaggcg gcgcgcacac tcgagaagct tggcgatcgc cggctgctgc
     6301 acgactgtca gcagatgctc atgcgcctgg gcggtgggac cactgtcact tccagcgaat
     6361 tcgattacaa ggatgacgac gataagtgag gatcccgcga ctctagataa ttctaccggg
     6421 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     6481 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     6541 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     6601 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     6661 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     6721 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     6781 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     6841 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     6901 catctccggg cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt
     6961 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     7021 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     7081 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     7141 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     7201 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     7261 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     7321 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     7381 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     7441 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     7501 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaccg cgtctggaac aatcaacctc
     7561 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     7621 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     7681 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     7741 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     7801 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     7861 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     7921 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     7981 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     8041 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     8101 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg
     8161 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     8221 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     8281 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag
     8341 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     8401 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     8461 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     8521 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     8581 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     8641 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     8701 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     8761 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     8821 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8881 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8941 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     9001 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt
     9061 gctagcgttt taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     9121 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     9181 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     9241 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     9301 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     9361 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     9421 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     9481 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     9541 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     9601 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     9661 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     9721 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     9781 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     9841 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     9901 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     9961 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    10021 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    10081 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    10141 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    10201 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    10261 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    10321 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    10381 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    10441 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    10501 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    10561 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    10621 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    10681 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    10741 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    10801 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    10861 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10921 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    10981 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    11041 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    11101 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    11161 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    11221 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
    11281 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    11341 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    11401 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
    11461 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
    11521 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
    11581 agtttttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
