LOCUS       V040135                 9559 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040135
VERSION     V040135
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..4234
                     /label="SF3A2(NM_007165)"
                     /note="SF3A2(NM_007165)"
                     /gene="SF3A2"
     CDS             4241..4267
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4271..4297
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4301..4327
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4347..4846
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             4867..5466
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5480..6068
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6275..6908
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7037..7053)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7061..7077
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7085..7115)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7130..7151
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7439..8027)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8198..9058)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9059..9163)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    9211..9345
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatggactt ccagcatcgc cccgggggca agaccgggag
     2881 cgggggcgtg gcctcctcct ccgagagcaa ccgtgaccgc agggagcgcc tccggcagct
     2941 ggccctggag accatcgaca tcaacaagga cccgtacttc atgaagaacc acctgggctc
     3001 ctatgaatgc aaactctgcc tgacacttca caacaatgag gggagctacc tggcacatac
     3061 gcaggggaag aagcaccaga ccaacctggc ccggcgagca gccaaggagg ccaaggaggc
     3121 ccctgcccag cccgcgcctg agaaggtcaa ggtggaggtg aagaagtttg tgaagatcgg
     3181 ccgcccgggc tacaaagtga ccaagcagag agactcggag atgggccagc agagcctcct
     3241 cttccagatt gactaccctg agatcgccga gggcatcatg ccacgtcacc gcttcatgtc
     3301 tgcgtacgag cagaggatcg agcctccgga ccggcgctgg cagtacctgc tcatggccgc
     3361 cgagccctac gagaccattg ccttcaaggt gccgagcaga gagatcgaca aggcggaggg
     3421 caagttctgg acacactgga accgggagac caagcagttc ttcctccagt tccactttaa
     3481 gatggagaag cccccggctc cacccagcct ccctgctggc ccccctgggg tgaagcggcc
     3541 tccacccccg ctgatgaacg gtctgccccc tcggccaccg ctgcctgagt ctttgccacc
     3601 gcccccgcca ggaggcctgc ctctgccacc catgcccccc acagggcctg cgccctcagg
     3661 gcccccggga ccaccccagc tacccccgcc agctccaggg gtccaccccc cggccccagt
     3721 ggtgcatccc cctgcatctg gggtccatcc cccagctcct ggcgtccacc ccccagctcc
     3781 tggcgtccat cccccagccc ctggggtcca cccaccaacc tctggggtcc accccccagc
     3841 tcctggagtc caccctccag cccccggggt tcacccacca gcccccggag tccacccacc
     3901 agcccctggg gttcacccac cagccccagg ggtccatcct cccccatcag cgggggttca
     3961 cccccaggcc ccgggggtgc acccagcagc ccccgccgtt caccctcagg ccccaggggt
     4021 gcacccacca gccccaggga tgcaccctca ggccccgggg gtccaccccc aacctcccgg
     4081 ggtccatccg tcggctcctg gggtccaccc tcagcctccg ggagttcacc cctcaaatcc
     4141 tggggtgcac cccccaactc ccatgccccc aatgctgagg cccccacttc cctccgaagg
     4201 cccagggaac atacctcccc ctcccccaac caacggatcc tacccatacg acgtacctga
     4261 ttatgcaact tatccttatg atgtacctga ttatgctagc tatccttatg atgtcccaga
     4321 ctacgcatag tctagataat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
     4381 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
     4441 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
     4501 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
     4561 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
     4621 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
     4681 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
     4741 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
     4801 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag
     4861 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     4921 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     4981 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     5041 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     5101 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     5161 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     5221 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     5281 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     5341 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     5401 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     5461 gcctgaccgc gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     5521 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     5581 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     5641 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     5701 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     5761 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     5821 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     5881 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     5941 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     6001 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     6061 ctccccgcct ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt
     6121 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     6181 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     6241 gatcttagcc actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga
     6301 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag
     6361 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta
     6421 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta
     6481 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt
     6541 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac
     6601 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc
     6661 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
     6721 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     6781 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     6841 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     6901 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     6961 aatatcagag agtgagaggc cttgacattg ctagcgtttt accgtcgacc tctagctaga
     7021 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     7081 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     7141 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     7201 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     7261 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     7321 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     7381 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     7441 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     7501 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     7561 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     7621 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     7681 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     7741 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     7801 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     7861 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     7921 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     7981 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8041 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     8101 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     8161 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     8221 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     8281 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     8341 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     8401 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     8461 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     8521 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     8581 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     8641 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     8701 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     8761 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     8821 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     8881 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     8941 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9001 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9061 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9121 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     9181 tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac
     9241 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     9301 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
     9361 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
     9421 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
     9481 atttgttaaa tatgtactac aaacttagta gtttttaaag aaattgtatt tgttaaatat
     9541 gtactacaaa cttagtagt
//
