LOCUS       V034270                11992 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034270
VERSION     V034270
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..6667
                     /label="Prdm16(NM_027504)"
                     /note="Prdm16(NM_027504)"
                     /gene="Prdm16"
     CDS             6674..6700
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             6704..6730
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             6734..6760
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        6780..7279
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             7300..7899
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7913..8501
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8708..9341
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(9470..9486)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9494..9510
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9518..9548)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9563..9584
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9872..10460)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10631..11491)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11492..11596)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11644..11778
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatgcgatc caaggcgagg gcgaggaagc tagccaaaag
     2881 tgacggtgac gttgtaaata atatgtatga acctgacccg gacctgctgg ccggccagag
     2941 tgccgaggag gagaccgaag acggcatcct gtcccccatc cccatggggc caccgtcccc
     3001 cttccccacc agcgaggact tcactcccaa ggagggctcg ccctatgagg ctcctgtcta
     3061 cattcctgaa gacattccaa tcccaccaga cttcgagcta cgagagtcct ccataccagg
     3121 agctggcctg gggatctggg ccaagcggaa gatggaaatc ggggagaggt ttggccccta
     3181 cgtggtgacg ccccgggccg cactgaagga ggccgacttt ggatgggaga tgctgacgga
     3241 tacagaggtg tcatcccagg agagctgcat caaaaagcag atctctgaag acttgggtag
     3301 cgagaagttc tgcgtggatg ccaatcaggc ggggtctggc agctggctca agtacatccg
     3361 tgtagcgtgt tcctgtgatg accaaaacct cgccatgtgt cagatcaacg aacagattta
     3421 ctataaagtc attaaggaca tcgagcctgg agaggaactg ttggtgcatg tgaaagaagg
     3481 tgcctactcc ttgggtgtca tggcccccag cttggatgag gaccccacat tccgctgtga
     3541 tgagtgtgat gagctcttcc agtgcaggct ggacctgagg cgccacaaga agtacgcgtg
     3601 cagctctgca ggagcccagc tctacgaggg cctaggggag gaactcaagc ccgagggcct
     3661 tggcgtgggc agcgacgggc aagcgcatga gtgcaaggat tgcgagcgga tgttccccaa
     3721 caagtacagc ttggagcaac acatgatcgt ccacacggaa gagcgtgagt acaaatgtga
     3781 ccagtgtccc aaggccttca actggaagtc caacctcatc cgccaccaga tgtctcacga
     3841 cagtggcaag cgcttcgaat gtgaaaactg tgtcaaggtg ttcacggacc ccagcaacct
     3901 ccagcgtcac atccgctcac agcatgtcgg tgcccgggcc catgcctgcc ctgactgtgg
     3961 caagaccttc gccacatcct ctggcctcaa acagcacaag catatccaca gcacggtgaa
     4021 gccattcata tgcgaggtct gccacaagtc ctacacgcag ttctccaacc tgtgccggca
     4081 caagcggatg cacgccgact gcaggacgca gatcaagtgc aaggactgtg ggcagatgtt
     4141 cagcactacc tcctccctca acaagcatcg gagattctgc gagggcaaga accattacac
     4201 gcctggcagc atcttcaccc caggcctgcc cttgaccccc agccccatga tggacaagac
     4261 aaaaccctcc ccgaccctca accacggggg cctaggcttc agcgagtact tcccctccag
     4321 acctcatcct gggagcctgc ccttctcggc tgctcctccg gccttccccg cactcactcc
     4381 gggcttcccg ggcatctttc ctccatccct gtacccacga ccacctctgc tacctcccac
     4441 gccgctgctc aagagccccc tgaaccacgc gcaggacgcc aagctaccca gcccgctggg
     4501 aaacccagcc ctgccccttg tctccgcggt cagcaatagc agccagggtg ccacagcggc
     4561 caccgggtca gaggagaaat ttgatggccg cttggaagac gcatatgcgg agaaggtcaa
     4621 aaataggagc cctgacatgt cggatggcag tgactttgag gatatcaaca ccacgaccgg
     4681 gacagacttg gacactacca cgggcacggg gtcagacctg gacagcgacc tggacagtga
     4741 cagagacaaa ggcaaggaca aggggaagcc agtggagagc aaacctgagt ttgggggtgc
     4801 atctgtgccc cctggggcca tgaacagtgt ggccgaggta ccggccttct actcacagca
     4861 ttccttcttc ccgccacccg aggaacagct gctgacggcc tcgggagctg ccggcgactc
     4921 catcaaggcc atcgcgtcca tcgcggagaa atacttcggt cctggcttca tgagcatgca
     4981 ggagaagaag ctgggctcac taccctacca ctccgtgttc cccttccagt tcctgcctaa
     5041 ctttccccac tccctctacc cctttacgga ccgagccctc gcccacaact tgctggtcaa
     5101 ggctgagcca aagtcacccc gggatgccct caaggtgggc ggccccagtg cggagtgccc
     5161 cttcgacctc accaccaaac caaaagaggc caaacccgcc ctgctcgcac ccaaggtccc
     5221 cctcatcccc tcatctggcg aggaacagcc actggacctg agcatcggca gcagggccag
     5281 ggcaagccag aacggaggtg gccgtgagcc gcggaagaac cacgtctacg gtgaacggaa
     5341 gccgggggtc agcgaggggc tgcctaaggt gtgcccagca cagctgcccc agcagccctc
     5401 cttgcattat gctaagcctt caccgttctt catggatccc atctacaggg tagaaaagcg
     5461 gaaggtggca gaccctgtgg gagtcctgaa agagaagtac ctgcggccgt ccccacttct
     5521 gttccacccc cagatgtcag ccatagaaac catgacggag aagctggaga gctttgcagc
     5581 catgaaggcc gactcaggca gctccctgca gcccctgcct caccacccgt tcaacttccg
     5641 ctccccaccc ccaacgctct cggatcccat cctcaggaag gggaaggaga gatacacgtg
     5701 caggtactgt ggcaagatct tccccagatc tgcaaatctc acaagacatc tgaggacaca
     5761 cacaggggag cagccataca ggtgcaagta ctgtgaccgg tcattcagca tctcctccaa
     5821 cctccagcgg cacgtgagga acatccacaa caaagagaag ccgttcaagt gccatctgtg
     5881 caaccgctgc ttcgggcagc agaccaacct agaccggcac ctgaagaagc acgaacacga
     5941 gggcgcacca gtgagccagc actccggggt gctcacgaac cacctgggca ccagcgcctc
     6001 ctcccccacc tccgagtcgg acaaccatgc acttttagat gagaaggaag attcttactt
     6061 ctccgagatc cgaaacttca tcgccaacag cgagatgaac caggcatcca ctcgaatgga
     6121 caaacggcct gagatccaag acctggacag caacccaccg tgtccaggct cagccagtgc
     6181 aaagccagag gacgtagagg aggaggaaga ggaggagctg gaggaagagg atgatgacag
     6241 cttagccggg aagtcacagg aggacacggt gtcccccaca cctgagcccc aaggagtcta
     6301 tgaagatgaa gaggatgagg aaccacccag cctgaccatg ggctttgacc atacccggag
     6361 gtgtgttgag gagcgaggag gcggcctgtt agctttggag ccgacgccga cctttgggaa
     6421 ggggctggat ctccgcagag cagctgagga agcatttgaa gttaaagatg tgcttaattc
     6481 caccttagat tctgaggttt taaaacaaac cctgtacagg caggctaaga accaggcata
     6541 tgcaatgatg ctgtccctct ctgaagacac tcctctccac gccccctccc agagctcact
     6601 ggatgcttgg ttgaacatca caggaccctc gtcagagtcc ggagccttta accccatcaa
     6661 ccacctcgga tcctacccat acgacgtacc tgattatgca acttatcctt atgatgtacc
     6721 tgattatgct agctatcctt atgatgtccc agactacgca tagtctagat aattctaccg
     6781 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     6841 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     6901 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     6961 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     7021 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     7081 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     7141 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     7201 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     7261 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg
     7321 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     7381 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     7441 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     7501 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     7561 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     7621 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     7681 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     7741 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     7801 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     7861 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac cgcgtctgga acaatcaacc
     7921 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     7981 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     8041 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     8101 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     8161 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     8221 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     8281 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     8341 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     8401 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     8461 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt
     8521 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     8581 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     8641 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     8701 aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     8761 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
     8821 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
     8881 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
     8941 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
     9001 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
     9061 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
     9121 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
     9181 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     9241 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     9301 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     9361 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca
     9421 ttgctagcgt tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     9481 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     9541 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     9601 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     9661 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     9721 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     9781 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     9841 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     9901 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     9961 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    10021 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    10081 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    10141 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    10201 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    10261 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
    10321 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    10381 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    10441 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    10501 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    10561 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    10621 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    10681 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    10741 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    10801 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    10861 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    10921 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    10981 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    11041 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    11101 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    11161 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    11221 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    11281 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    11341 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    11401 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    11461 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    11521 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    11581 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
    11641 atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    11701 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    11761 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
    11821 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
    11881 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
    11941 gtagttttta aagaaattgt atttgttaaa tatgtactac aaacttagta gt
//
