LOCUS       V021897                11374 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021897
VERSION     V021897
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2826..6113
                     /label="PTK2(NM_001352699)"
                     /note="PTK2(NM_001352699)"
                     /gene="PTK2"
     CDS             6111..6131
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHHH"
                     /label="7xHis"
     promoter        6162..6661
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6682..7281
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7295..7883
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8090..8723
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8852..8868)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8876..8892
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8900..8930)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8945..8966
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9254..9842)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10013..10873)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10874..10978)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11026..11160
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ccaccatggc agctgcttac cttgacccca acttgaatca cacaccaaat tcgagtacta
     2881 agactcacct gggtactggt atggaacgtt ctcctggtgc aatggagcga gtattaaagg
     2941 tctttcatta ttttgaaagc aatagtgagc caaccacctg ggccagtatt atcaggcatg
     3001 gagatgctac tgatgtcagg ggcatcattc agaagatagt ggacagtcac aaagtaaagc
     3061 atgtggcctg ctatggattc cgcctcagtc acctgcggtc agaggaggtt cactggcttc
     3121 acgtggatat gggcgtctcc agtgtgaggg agaagtatga gcttgctcac ccaccagagg
     3181 agtggaaata tgaattgaga attcgttatt tgccaaaagg atttctaaac cagtttactg
     3241 aagataagcc aactttgaat ttcttctatc aacaggtgaa gagcgattat atgttagaga
     3301 tagctgatca agtggaccag gaaattgctt tgaagttggg ttgtctagaa atacggcgat
     3361 catactggga gatgcggggc aatgcactag aaaagaagtc taactatgaa gtattagaaa
     3421 aagatgttgg tttaaagcga ttttttccta agagtttact ggattctgtc aaggccaaaa
     3481 cactaagaaa actgatccaa caaacattta gacaatttgc caaccttaat agagaagaaa
     3541 gtattctgaa attctttgag atcctgtctc cagtctacag atttgataag gaatgcttca
     3601 agtgtgctct tggttcaagc tggattattt cagtggaact ggcaatcggc ccagaagaag
     3661 gaatcagtta cctaacggac aagggctgca atcccacaca tcttgctgac ttcactcaag
     3721 tgcaaaccat tcagtattca aacagtgaag acaaggacag aaaaggaatg ctacaactaa
     3781 aaatagcagg tgcacccgag cctctgacag tgacggcacc atccctaacc attgcggaga
     3841 atatggctga cctaatagat gggtactgcc ggctggtgaa tggaacctcg cagtcattta
     3901 tcatcagacc tcagaaagaa ggtgaacggg ctttgccatc aataccaaag ttggccaaca
     3961 gcgaaaagca aggcatgcgg acacacgccg tctctgtgtc aggagtcagt cactgccaac
     4021 ataaagttaa gaaagctagg cgctttctcc ctttggtctt ctgttcccat gatcctcctt
     4081 ctacggatga aattagtggg gacgaaacag atgattatgc tgagattata gatgaagaag
     4141 atacttacac catgccctca aaaagctatg gaatagatga agccagggat tatgagattc
     4201 aaagagaaag aatagaactt ggacgatgta ttggagaagg ccaatttgga gatgtacatc
     4261 aaggcattta tatgagtcca gagaatccag ctttggcggt tgcaattaaa acatgtaaaa
     4321 actgtacttc ggacagcgtg agagagaaat ttcttcaaga agccttaaca atgcgtcagt
     4381 ttgaccatcc tcatattgtg aagctgattg gagtcatcac agagaatcct gtctggataa
     4441 tcatggagct gtgcacactt ggagagctga ggtcattttt gcaagtaagg aaatacagtt
     4501 tggatctagc atctttgatc ctgtatgcct atcagcttag tacagctctt gcatatctag
     4561 agagcaaaag atttgtacac agggacattg ctgctcggaa tgttctggtg tcctcaaatg
     4621 attgtgtaaa attaggagac tttggattat cccgatatat ggaagatagt acttactaca
     4681 aagcttccaa aggaaaattg cctattaaat ggatggctcc agagtcaatc aattttcgac
     4741 gttttacctc agctagtgac gtatggatgt ttggtgtgtg tatgtgggag atactgatgc
     4801 atggtgtgaa gccttttcaa ggagtgaaga acaatgatgt aatcggtcga attgaaaatg
     4861 gggaaagatt accaatgcct ccaaattgtc ctcctaccct ctacagcctt atgacgaaat
     4921 gctgggccta tgaccccagc aggcggccca ggtttactga acttaaagct cagctcagca
     4981 caatcctgga ggaagagaag gctcagcaag aagagcgcat gaggatggag tccagaagac
     5041 aggccacagt gtcctgggac tccggagggt ctgatgaagc accgcccaag cccagcagac
     5101 cgggttatcc cagtccgagg tccagcgaag gattttatcc cagcccacag cacatggtac
     5161 aaaccaatca ttaccaggtt tctggctacc ctggttcaca tggaatcaca gccatggctg
     5221 gcagcatcta tccaggtcag gcatctcttt tggaccaaac agattcatgg aatcatagac
     5281 ctcaggagat agcaatgtgg cagcccaatg tggaggactc tacagtattg gacctgcgag
     5341 ggattgggca agtgttgcca acccatctga tggaagagcg tctaatccga cagcaacagg
     5401 aaatggaaga agatcagcgc tggctggaaa aagaggaaag atttctgaaa cctgatgtga
     5461 gactctctcg aggcagtatt gacagggagg atggaagtct tcagggtccg attggaaacc
     5521 aacatatata tcagcctgtg ggtaaaccag atcctgcagc tccaccaaag aaaccgcctc
     5581 gccctggagc tcccggtcat ctgggaagcc ttgccagcct cagcagccct gctgacagct
     5641 acaacgaggg tgtcaagcca tggaggcttc agccccagga aatcagcccc cctcctactg
     5701 ccaacctgga ccggtcgaat gataaggtgt acgagaatgt gacgggcctg gtgaaagctg
     5761 tcatcgagat gtccagtaaa atccagccag ccccaccaga ggagtatgtc cctatggtga
     5821 aggaagtcgg cttggccctg aggacattat tggccactgt ggatgagacc attcccctcc
     5881 taccagccag cacccaccga gagattgaga tggcacagaa gctattgaac tctgacctgg
     5941 gtgagctcat caacaagatg aaactggccc agcagtatgt catgaccagc ctccagcaag
     6001 agtacaaaaa gcaaatgctg actgctgctc acgccctggc tgtggatgcc aaaaacttac
     6061 tcgatgtcat tgaccaagca agactgaaaa tgcttgggca gacgagacca caccaccatc
     6121 accatcacca ttaggatccc gcgactctag ataattctac cgggtagggg aggcgctttt
     6181 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag
     6241 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
     6301 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
     6361 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
     6421 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
     6481 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct
     6541 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg
     6601 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc
     6661 gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     6721 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     6781 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     6841 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     6901 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     6961 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     7021 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     7081 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     7141 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     7201 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     7261 acccgcaagc ccggtgcctg accgcgtctg gaacaatcaa cctctggatt acaaaatttg
     7321 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     7381 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     7441 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     7501 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     7561 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     7621 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     7681 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     7741 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     7801 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     7861 ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat
     7921 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     7981 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     8041 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta
     8101 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     8161 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
     8221 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
     8281 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
     8341 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
     8401 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
     8461 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
     8521 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
     8581 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     8641 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     8701 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     8761 acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc gttttaccgt
     8821 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     8881 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     8941 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     9001 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     9061 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     9121 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     9181 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     9241 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     9301 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     9361 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     9421 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     9481 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     9541 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     9601 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     9661 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     9721 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     9781 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     9841 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     9901 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     9961 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    10021 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    10081 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    10141 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    10201 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    10261 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    10321 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    10381 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    10441 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    10501 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    10561 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    10621 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    10681 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    10741 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    10801 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    10861 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    10921 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    10981 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca
    11041 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    11101 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
    11161 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
    11221 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca
    11281 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagtttt taaagaaatt
    11341 gtatttgtta aatatgtact acaaacttag tagt
//
