LOCUS V021897 11374 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V021897 VERSION V021897 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2826..6113 /label="PTK2(NM_001352699)" /note="PTK2(NM_001352699)" /gene="PTK2" CDS 6111..6131 /codon_start=1 /product="6xHis affinity tag" /transl_table=1 /translation="HHHHHHH" /label="7xHis" promoter 6162..6661 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 6682..7281 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 7295..7883 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 8090..8723 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(8852..8868) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8876..8892 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8900..8930) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8945..8966 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(9254..9842) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(10013..10873) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10874..10978) /gene="bla" /label="AmpR promoter" polyA_signal 11026..11160 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ccaccatggc agctgcttac cttgacccca acttgaatca cacaccaaat tcgagtacta 2881 agactcacct gggtactggt atggaacgtt ctcctggtgc aatggagcga gtattaaagg 2941 tctttcatta ttttgaaagc aatagtgagc caaccacctg ggccagtatt atcaggcatg 3001 gagatgctac tgatgtcagg ggcatcattc agaagatagt ggacagtcac aaagtaaagc 3061 atgtggcctg ctatggattc cgcctcagtc acctgcggtc agaggaggtt cactggcttc 3121 acgtggatat gggcgtctcc agtgtgaggg agaagtatga gcttgctcac ccaccagagg 3181 agtggaaata tgaattgaga attcgttatt tgccaaaagg atttctaaac cagtttactg 3241 aagataagcc aactttgaat ttcttctatc aacaggtgaa gagcgattat atgttagaga 3301 tagctgatca agtggaccag gaaattgctt tgaagttggg ttgtctagaa atacggcgat 3361 catactggga gatgcggggc aatgcactag aaaagaagtc taactatgaa gtattagaaa 3421 aagatgttgg tttaaagcga ttttttccta agagtttact ggattctgtc aaggccaaaa 3481 cactaagaaa actgatccaa caaacattta gacaatttgc caaccttaat agagaagaaa 3541 gtattctgaa attctttgag atcctgtctc cagtctacag atttgataag gaatgcttca 3601 agtgtgctct tggttcaagc tggattattt cagtggaact ggcaatcggc ccagaagaag 3661 gaatcagtta cctaacggac aagggctgca atcccacaca tcttgctgac ttcactcaag 3721 tgcaaaccat tcagtattca aacagtgaag acaaggacag aaaaggaatg ctacaactaa 3781 aaatagcagg tgcacccgag cctctgacag tgacggcacc atccctaacc attgcggaga 3841 atatggctga cctaatagat gggtactgcc ggctggtgaa tggaacctcg cagtcattta 3901 tcatcagacc tcagaaagaa ggtgaacggg ctttgccatc aataccaaag ttggccaaca 3961 gcgaaaagca aggcatgcgg acacacgccg tctctgtgtc aggagtcagt cactgccaac 4021 ataaagttaa gaaagctagg cgctttctcc ctttggtctt ctgttcccat gatcctcctt 4081 ctacggatga aattagtggg gacgaaacag atgattatgc tgagattata gatgaagaag 4141 atacttacac catgccctca aaaagctatg gaatagatga agccagggat tatgagattc 4201 aaagagaaag aatagaactt ggacgatgta ttggagaagg ccaatttgga gatgtacatc 4261 aaggcattta tatgagtcca gagaatccag ctttggcggt tgcaattaaa acatgtaaaa 4321 actgtacttc ggacagcgtg agagagaaat ttcttcaaga agccttaaca atgcgtcagt 4381 ttgaccatcc tcatattgtg aagctgattg gagtcatcac agagaatcct gtctggataa 4441 tcatggagct gtgcacactt ggagagctga ggtcattttt gcaagtaagg aaatacagtt 4501 tggatctagc atctttgatc ctgtatgcct atcagcttag tacagctctt gcatatctag 4561 agagcaaaag atttgtacac agggacattg ctgctcggaa tgttctggtg tcctcaaatg 4621 attgtgtaaa attaggagac tttggattat cccgatatat ggaagatagt acttactaca 4681 aagcttccaa aggaaaattg cctattaaat ggatggctcc agagtcaatc aattttcgac 4741 gttttacctc agctagtgac gtatggatgt ttggtgtgtg tatgtgggag atactgatgc 4801 atggtgtgaa gccttttcaa ggagtgaaga acaatgatgt aatcggtcga attgaaaatg 4861 gggaaagatt accaatgcct ccaaattgtc ctcctaccct ctacagcctt atgacgaaat 4921 gctgggccta tgaccccagc aggcggccca ggtttactga acttaaagct cagctcagca 4981 caatcctgga ggaagagaag gctcagcaag aagagcgcat gaggatggag tccagaagac 5041 aggccacagt gtcctgggac tccggagggt ctgatgaagc accgcccaag cccagcagac 5101 cgggttatcc cagtccgagg tccagcgaag gattttatcc cagcccacag cacatggtac 5161 aaaccaatca ttaccaggtt tctggctacc ctggttcaca tggaatcaca gccatggctg 5221 gcagcatcta tccaggtcag gcatctcttt tggaccaaac agattcatgg aatcatagac 5281 ctcaggagat agcaatgtgg cagcccaatg tggaggactc tacagtattg gacctgcgag 5341 ggattgggca agtgttgcca acccatctga tggaagagcg tctaatccga cagcaacagg 5401 aaatggaaga agatcagcgc tggctggaaa aagaggaaag atttctgaaa cctgatgtga 5461 gactctctcg aggcagtatt gacagggagg atggaagtct tcagggtccg attggaaacc 5521 aacatatata tcagcctgtg ggtaaaccag atcctgcagc tccaccaaag aaaccgcctc 5581 gccctggagc tcccggtcat ctgggaagcc ttgccagcct cagcagccct gctgacagct 5641 acaacgaggg tgtcaagcca tggaggcttc agccccagga aatcagcccc cctcctactg 5701 ccaacctgga ccggtcgaat gataaggtgt acgagaatgt gacgggcctg gtgaaagctg 5761 tcatcgagat gtccagtaaa atccagccag ccccaccaga ggagtatgtc cctatggtga 5821 aggaagtcgg cttggccctg aggacattat tggccactgt ggatgagacc attcccctcc 5881 taccagccag cacccaccga gagattgaga tggcacagaa gctattgaac tctgacctgg 5941 gtgagctcat caacaagatg aaactggccc agcagtatgt catgaccagc ctccagcaag 6001 agtacaaaaa gcaaatgctg actgctgctc acgccctggc tgtggatgcc aaaaacttac 6061 tcgatgtcat tgaccaagca agactgaaaa tgcttgggca gacgagacca caccaccatc 6121 accatcacca ttaggatccc gcgactctag ataattctac cgggtagggg aggcgctttt 6181 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag 6241 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt 6301 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc 6361 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca 6421 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca 6481 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct 6541 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg 6601 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc 6661 gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc 6721 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg 6781 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc 6841 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg 6901 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg 6961 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg 7021 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac 7081 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc 7141 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg 7201 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg 7261 acccgcaagc ccggtgcctg accgcgtctg gaacaatcaa cctctggatt acaaaatttg 7321 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 7381 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 7441 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 7501 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 7561 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 7621 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 7681 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg 7741 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 7801 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat 7861 ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat 7921 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca 7981 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact 8041 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta 8101 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac 8161 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga 8221 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag 8281 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg 8341 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg 8401 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt 8461 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa 8521 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct 8581 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 8641 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 8701 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 8761 acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc gttttaccgt 8821 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 8881 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 8941 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 9001 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 9061 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 9121 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 9181 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 9241 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 9301 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 9361 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 9421 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 9481 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 9541 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 9601 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 9661 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 9721 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 9781 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 9841 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 9901 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 9961 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 10021 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 10081 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 10141 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 10201 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 10261 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 10321 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 10381 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 10441 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 10501 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 10561 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 10621 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 10681 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 10741 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 10801 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 10861 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 10921 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 10981 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca 11041 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 11101 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc 11161 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc 11221 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca 11281 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagtttt taaagaaatt 11341 gtatttgtta aatatgtact acaaacttag tagt //